دورية أكاديمية
A SEPALLATA1-like gene of Isatis indigotica Fort. regulates flowering time and specifies floral organs.
| العنوان: | A SEPALLATA1-like gene of Isatis indigotica Fort. regulates flowering time and specifies floral organs. |
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| المؤلفون: | Ma YQ; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China., Pu ZQ; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China., Zhang L; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China., Lu MX; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China., Zhu Y; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China., Hao CY; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China., Xu ZQ; Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi'an 710069, People's Republic of China. Electronic address: ziqinxu@nwu.edu.cn. |
| المصدر: | Gene [Gene] 2019 Sep 10; Vol. 713, pp. 143974. Date of Electronic Publication: 2019 Jul 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland, 1976- |
| مواضيع طبية MeSH: | Arabidopsis/*growth & development , Flowers/*growth & development , Forkhead Transcription Factors/*genetics , Isatis/*growth & development , Plant Proteins/*genetics , Plants, Genetically Modified/*growth & development , Schizosaccharomyces pombe Proteins/*genetics, Amino Acid Sequence ; Arabidopsis/genetics ; Flowers/genetics ; Gene Expression Regulation, Plant ; Isatis/genetics ; MADS Domain Proteins/genetics ; Phenotype ; Plants, Genetically Modified/genetics ; Sequence Homology |
| مستخلص: | An orthologous gene of SEPALLATA1, designated as IiSEP1, was isolated from Isatis indigotica. The genomic DNA of IiSEP1 is 3.1 Kb in length. The full-length cDNA of IiSEP1 is 1481 bp and contains a 756 bp ORF encoding a 251-amino-acid protein. Sequence comparison revealed that IiSEP1 belonged to the MADS-box gene family. IiSEP1 contains 7 exons and 6 introns, showing similar exon-intron structure with Arabidopsis SEP1. Phylogenetic analysis suggested that IiSEP1 belonged to AGL2/SEP subfamily and was likely to be an I. indigotica ortholog of Arabidopsis SEP1. Quantitative real-time PCR showed that IiSEP1 was predominantly expressed in the reproductive organs. Ectopic expression of IiSEP1 in Arabidopsis resulted in early flowering, accompanied with the reduction of inflorescence number and the production of terminal flower on the top of the main stems. Moreover, IiSEP1 overexpressing flowers generated numerous variations in phenotype. The sepals were changed into petal-sepal mosaic structures or displayed carpelloid features, and transparent ovules were formed in internal surface of these sepals. In addition, some flowers were constituted by sepals and pistil, but lacked petals and stamens. Taken together, IiSEP1 might play important roles in reproductive growth of I. indigotica and could affect the morphogenesis of flowers and fruits. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Arabidopsis thaliana; Flower development; IiSEP1; Isatis indigotica Fort.; MADS-box gene |
| المشرفين على المادة: | 0 (Forkhead Transcription Factors) 0 (MADS Domain Proteins) 0 (Plant Proteins) 0 (Schizosaccharomyces pombe Proteins) 0 (sep1 protein, S pombe) |
| تواريخ الأحداث: | Date Created: 20190714 Date Completed: 20190809 Latest Revision: 20190809 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.gene.2019.143974 |
| PMID: | 31301484 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0038 |
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| DOI: | 10.1016/j.gene.2019.143974 |