دورية أكاديمية
Microbiological and environmental assessment of human oral dental plaque isolates.
| العنوان: | Microbiological and environmental assessment of human oral dental plaque isolates. |
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| المؤلفون: | Elgamily HM; Restorative and Dental Materials Department, Oral and Dental Research Division, National Research Centre, 33 El-Buhouth St. (Former El Tahrir St), 12622, Dokki, Cairo, Egypt., Gamal AA; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, 33 El-Buhouth St. (Former El Tahrir St), 12622, Dokki, Cairo, Egypt., Saleh SAA; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, 33 El-Buhouth St. (Former El Tahrir St), 12622, Dokki, Cairo, Egypt., Abdel Wahab WA; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, 33 El-Buhouth St. (Former El Tahrir St), 12622, Dokki, Cairo, Egypt., Hashem AM; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, 33 El-Buhouth St. (Former El Tahrir St), 12622, Dokki, Cairo, Egypt., Esawy MA; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, 33 El-Buhouth St. (Former El Tahrir St), 12622, Dokki, Cairo, Egypt. Electronic address: ma.esawy@nrc.sci.eg. |
| المصدر: | Microbial pathogenesis [Microb Pathog] 2019 Oct; Vol. 135, pp. 103626. Date of Electronic Publication: 2019 Jul 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Orlando : Academic Press, c1986- |
| مواضيع طبية MeSH: | Bacteria/*classification , Bacteria/*isolation & purification , Dental Plaque/*microbiology , Streptococcus mutans/*isolation & purification, Animals ; Anti-Bacterial Agents/pharmacology ; Bacillaceae/isolation & purification ; Bacillus/isolation & purification ; Bacillus subtilis/isolation & purification ; Bacteria/drug effects ; Bacteria/genetics ; Bacterial Adhesion ; Cell Line ; Dental Caries/microbiology ; Dextrans/metabolism ; Glucosyltransferases ; Hexosyltransferases ; Humans ; Hydrogen-Ion Concentration ; Lactic Acid/metabolism ; Microbial Sensitivity Tests ; Mouth/microbiology ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Streptococcus mutans/drug effects |
| مستخلص: | Plaque-related diseases are amongst the most common ailments of the oral cavity. Streptococcus mutans is the causal agent of dental caries in animals and humans and is responsible for the formation and accumulation of plaques. This study aimed to identify and evaluate the role of the dental plaque isolates and its surrounding environment in plaque formation or inhibition. The study started with the identification of human dental plaque isolates from high caries index patients based on 16S rRNA and Mitis salivarius bacitracin agar (MSB) was used for S. mutans growing. Unexpectedly, the Streptococcus mutans was completely absent. The disc diffusion assay recorded that all the isolates had antimicrobial activity against the S. mutans growth. Enzymes assay revealed that the isolates produced dextransucrase, levansucrase and levanase activity with wide variation degrees. Also, the lactic acid production assay was done based in pH shift assessment. The highest pH shift and dextran yield were detected by the isolates Bacillus subtilis_AG1 and Bacillus mojavensis_AG3. The adherence test revealed that Lysinibacillus cresolivorans_W2 (MK411028) recorded the highest adhesion property (60%). Oligo- and polysaccharides were synthesized by the action of dextransucrase enzyme and their cytotoxicity tests were negative. Dextran with a molecular weight (117521 Da) recorded the highest antimicrobial efficacy against Bacillus subtilis_AG1 and Bacillusmojavensis_AG3 (65%, 63.5%) respectively. The results concluded that the dextran was the most important factor causing the dental plaque pathogenicity. Also, oral oligo- and polysaccharides might play a role in dental plaque control. (Copyright © 2019 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antimicrobial activity; Bacillus mojavensis; Bacillus subtilis; Dental plaque; Lysinibacillus cresolivorans; Oligo- and polysaccharides; Streptococcus mutans |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Dextrans) 0 (RNA, Ribosomal, 16S) 33X04XA5AT (Lactic Acid) EC 2.4.1.- (Glucosyltransferases) EC 2.4.1.- (Hexosyltransferases) EC 2.4.1.10 (levansucrase) EC 2.4.1.5 (dextransucrase) |
| SCR Organism: | Bacillus mojavensis; Lysinibacillus cresolivorans |
| تواريخ الأحداث: | Date Created: 20190721 Date Completed: 20200113 Latest Revision: 20200113 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.micpath.2019.103626 |
| PMID: | 31325573 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-1208 |
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| DOI: | 10.1016/j.micpath.2019.103626 |