دورية أكاديمية
أعرض في EDS

Genome-based insights into the resistomes and mobilomes of two Providencia rettgeri strains isolated from wound infections in Madagascar.

التفاصيل البيبلوغرافية
العنوان: Genome-based insights into the resistomes and mobilomes of two Providencia rettgeri strains isolated from wound infections in Madagascar.
المؤلفون: Tchuinte PLS; Institut Pasteur de Madagascar, Antananarivo, Madagascar. Electronic address: simolandrie@yahoo.fr., Rabenandrasana MAN; Institut Pasteur de Madagascar, Antananarivo, Madagascar., Ramparany L; Institut Pasteur de Madagascar, Antananarivo, Madagascar., Ratsima E; Institut Pasteur de Madagascar, Antananarivo, Madagascar., Enouf V; Institut Pasteur, Pasteur International Bioresources network (PIBnet), Plateforme de Microbiologie Mutualisée (P2M), Paris, France., Randrianirina F; Institut Pasteur de Madagascar, Antananarivo, Madagascar., Collard JM; Institut Pasteur de Madagascar, Antananarivo, Madagascar.
المصدر: Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2020 Mar; Vol. 20, pp. 178-182. Date of Electronic Publication: 2019 Jul 17.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer Country of Publication: Netherlands NLM ID: 101622459 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-7173 (Electronic) Linking ISSN: 22137165 NLM ISO Abbreviation: J Glob Antimicrob Resist Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer
مواضيع طبية MeSH: Drug Resistance, Multiple, Bacterial*, Anti-Bacterial Agents/*pharmacology , Providencia/*classification , Whole Genome Sequencing/*methods , Wound Infection/*microbiology, Gene Transfer, Horizontal ; Genome, Bacterial ; Genomic Islands ; High-Throughput Nucleotide Sequencing ; Humans ; Interspersed Repetitive Sequences ; Madagascar ; Microbial Sensitivity Tests ; Phylogeny ; Plasmids/genetics ; Providencia/drug effects ; Providencia/genetics ; Providencia/isolation & purification ; Spain ; United States
مستخلص: Objectives: A molecular analysis was performed of two Providencia rettgeri (P. rettgeri) strains (Pr 297 and Pr 269) collected in 2007 and 2009 from wound swabs of patients admitted to the intensive care units at Joseph Ravoangy Andrianavalona hospital and the Military Hospital in Antananarivo, Madagascar.
Methods: The two P. rettgeri isolates were subjected to susceptibility testing. Whole genome sequencing was performed to characterise the antibiotic resistance genes, genomic islands and mobilomes (integrons, plasmids and insertion sequences).
Results: All isolates were found to be multidrug-resistant. Antibiotic-resistant genes described were amongst eight different classes of antimicrobial agents. Thirty insertion sequences and twelve genomic islands were predicted in each genome. Class 1 and class 2 integrons were found in both genomes, with gene cassette regions encompassing arr-2 - cmlA5 - bla OXA-10 - ant (3")-Ia and dfrA1 - sat2 - ant (3")-Ia - orfX, respectively. IncA/C2, ColM and ColE1-like plasmids were described harbouring bla CMY-30 , qnrD and aac(6')-Ib-cr4 genes, respectively. Phylogenetic analysis showed that Pr 297 and Pr 269 isolates were genetically identical and clustered with P. rettgeri strains described in the USA and Spain.
Conclusions: It is believed that this is the first molecular characterisation of wound infection pathogens from Madagascan patients and the first description of P. rettgeri co-producing CMY-30, OXA-10 and AAC(6')-Ib-cr4 enzymes. The diversity of the resistance determinants and mobile genetic elements was probably due to extensive horizontal gene transfer events, highlighting the need to conduct further molecular monitoring studies to understand the genomic plasticity of resistant bacteria in Madagascan hospitals.
(Copyright © 2019 International Society for Antimicrobial Chemotherapy. Published by Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Antimicrobial resistance; Intensive care unit; Providencia rettgeri; Wound infections
المشرفين على المادة: 0 (Anti-Bacterial Agents)
SCR Organism: Providencia rettgeri
تواريخ الأحداث: Date Created: 20190721 Date Completed: 20210210 Latest Revision: 20210210
رمز التحديث: 20240513
DOI: 10.1016/j.jgar.2019.07.013
PMID: 31325615
قاعدة البيانات: MEDLINE
الوصف
تدمد:2213-7173
DOI:10.1016/j.jgar.2019.07.013