دورية أكاديمية
The diagnostic performance of rapid diagnostic tests and microscopy for malaria diagnosis in eastern Sudan using a nested polymerase chain reaction assay as a reference standard.
| العنوان: | The diagnostic performance of rapid diagnostic tests and microscopy for malaria diagnosis in eastern Sudan using a nested polymerase chain reaction assay as a reference standard. |
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| المؤلفون: | Abdalla ZA; University of Bahri, Bahri, Sudan., Rahma NA; University of Bahri, Bahri, Sudan., Hassan EE; College of Applied Medical Sciences, Najran University, Najran, Saudi Arabia., Abdallah TM; Faculty of Medicine, Kassala University, PO Box 496, Kassala, Sudan., Hamad HE; Department of Immunology and Biotechnology, Tropical Medicine Research Institute, National Centre for Research, Khartoum, Sudan., Omer SA; Department of Immunology and Biotechnology, Tropical Medicine Research Institute, National Centre for Research, Khartoum, Sudan., Adam I; Faculty of Medicine, University of Khartoum, PO Box 102, Khartoum, Sudan. |
| المصدر: | Transactions of the Royal Society of Tropical Medicine and Hygiene [Trans R Soc Trop Med Hyg] 2019 Nov 01; Vol. 113 (11), pp. 701-705. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 7506129 Publication Model: Print Cited Medium: Internet ISSN: 1878-3503 (Electronic) Linking ISSN: 00359203 NLM ISO Abbreviation: Trans R Soc Trop Med Hyg Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2013- : Oxford : Oxford University Press Original Publication: 1920- : London : Royal Society of Tropical Medicine and Hygiene |
| مواضيع طبية MeSH: | Biological Assay/*standards , Diagnostic Tests, Routine/*standards , Malaria, Falciparum/*diagnosis , Malaria, Falciparum/*genetics , Microscopy/*standards , Plasmodium falciparum/*genetics , Polymerase Chain Reaction/*standards, Adolescent ; Adult ; Biological Assay/statistics & numerical data ; Child ; Cross-Sectional Studies ; Diagnostic Tests, Routine/statistics & numerical data ; Early Diagnosis ; Female ; Humans ; Malaria, Falciparum/epidemiology ; Male ; Microscopy/statistics & numerical data ; Middle Aged ; Plasmodium falciparum/isolation & purification ; Polymerase Chain Reaction/statistics & numerical data ; Prevalence ; Reproducibility of Results ; Sudan/epidemiology ; Young Adult |
| مستخلص: | Background: Accurate diagnosis of malaria infection is essential for successful control and management of the disease. Both microscopy and rapid diagnostic tests (RDTs) are recommended for malaria diagnosis, however, RDTs are more commonly used. The aim of the current study was to assess the performance of microscopy and RDTs in the diagnosis of Plasmodium falciparum infection using a nested polymerase chain reaction (PCR) assay as the gold standard. Methods: A cross-sectional study was carried out in Kassala Hospital, eastern Sudan. A total of 341 febrile participants of all ages were recruited. Blood specimens were collected and malaria testing was performed using an RDT (SD Bioline Malaria Ag Pf), microscopy and nested PCR. The sensitivity, specificity, positive and negative predictive values (PPV and NPV, respectively) of microscopy and the RDT were investigated. Results: The prevalence of P. falciparum malaria infections in this study was 22.9%, 24.3% and 26.7% by PCR, microscopy and RDT, respectively. Compared with microscopy, the RDT had slightly higher sensitivity (80.7% vs 74.3%; p=0.442), equivalent specificity (89.3% vs 90.4%), a similar PPV (69.2% vs 69.8%) and a higher NPV (94.0% vs 92.2%). Conclusions: The diagnostic performance of the RDT was better than that of microscopy in the diagnosis of P. falciparum malaria when nested PCR was used as the gold standard. (© The Author(s) 2019. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| فهرسة مساهمة: | Keywords: Plasmodium falciparum; RDT; malaria; microscopy; nested PCR |
| تواريخ الأحداث: | Date Created: 20190724 Date Completed: 20200921 Latest Revision: 20200921 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/trstmh/trz069 |
| PMID: | 31334809 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-3503 |
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| DOI: | 10.1093/trstmh/trz069 |