دورية أكاديمية
White matter mean diffusivity correlates with myelination in tuberous sclerosis complex.
العنوان: | White matter mean diffusivity correlates with myelination in tuberous sclerosis complex. |
---|---|
المؤلفون: | Peters JM; Division of Epilepsy and Clinical Neurophysiology, Department of Neurology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts.; Computational Radiology Laboratory, Department of Radiology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Struyven RR; Computational Radiology Laboratory, Department of Radiology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Prohl AK; Computational Radiology Laboratory, Department of Radiology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Vasung L; Computational Radiology Laboratory, Department of Radiology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Stajduhar A; Croatian Institute for Brain Research and Center of Research Excellence for Basic, Clinical and Translational Neuroscience, University of Zagreb, Zagreb, Croatia., Taquet M; Computational Radiology Laboratory, Department of Radiology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Bushman JJ; Computational Radiology Laboratory, Department of Radiology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Lidov H; Division of Neuropathology, Department of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Singh JM; Computational Radiology Laboratory, Department of Radiology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Scherrer B; Computational Radiology Laboratory, Department of Radiology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Madsen JR; Department of Neurosurgery, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Prabhu SP; Computational Radiology Laboratory, Department of Radiology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Sahin M; Translational Neuroscience Center, Department of Neurology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Afacan O; Computational Radiology Laboratory, Department of Radiology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts., Warfield SK; Computational Radiology Laboratory, Department of Radiology, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts. |
المصدر: | Annals of clinical and translational neurology [Ann Clin Transl Neurol] 2019 Jul; Vol. 6 (7), pp. 1178-1190. Date of Electronic Publication: 2019 Jun 23. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: Wiley Periodicals, Inc on behalf of American Neurological Association Country of Publication: United States NLM ID: 101623278 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2328-9503 (Electronic) Linking ISSN: 23289503 NLM ISO Abbreviation: Ann Clin Transl Neurol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: [Hoboken, NJ] : Wiley Periodicals, Inc on behalf of American Neurological Association, [2014]- |
مواضيع طبية MeSH: | Myelin Sheath/*pathology , Tuberous Sclerosis/*diagnostic imaging , Tuberous Sclerosis/*pathology , White Matter/*diagnostic imaging , White Matter/*pathology, Anisotropy ; Brain/pathology ; Cerebral Cortex/diagnostic imaging ; Cerebral Cortex/pathology ; Diffusion Tensor Imaging/methods ; Female ; Gliosis/pathology ; Humans ; Infant ; Infant, Newborn ; Male ; Neurons/pathology |
مستخلص: | Objective: Diffusion tensor imaging (DTI) of the white matter is a biomarker for neurological disease burden in tuberous sclerosis complex (TSC). To clarify the basis of abnormal diffusion in TSC, we correlated ex vivo high-resolution diffusion imaging with histopathology in four tissue types: cortex, tuber, perituber, and white matter. Methods: Surgical specimens of three children with TSC were scanned in a 3T or 7T MRI with a structural image isotropic resolution of 137-300 micron, and diffusion image isotropic resolution of 270-1,000 micron. We stained for myelin (luxol fast blue, LFB), gliosis (glial fibrillary acidic protein, GFAP), and neurons (NeuN) and registered the digitized histopathology slides (0.686 micron resolution) to MRI for visual comparison. We then performed colocalization analysis in four tissue types in each specimen. Finally, we applied a linear mixed model (LMM) for pooled analysis across the three specimens. Results: In white matter and perituber regions, LFB optical density measures correlated with fractional anisotropy (FA) and inversely with mean diffusivity (MD). In white matter only, GFAP correlated with MD, and inversely with FA. In tubers and in the cortex, there was little variation in mean LFB and GFAP signal intensity, and no correlation with MRI metrics. Neuronal density correlated with MD. In the analysis of the combined specimens, the most robust correlation was between white matter MD and LFB metrics. Interpretation: In TSC, diffusion imaging abnormalities in microscopic tissue types correspond to specific histopathological markers. Across all specimens, white matter diffusivity correlates with myelination. (© 2019 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association.) |
References: | Ann Neurol. 2017 Sep;82(3):331-341. (PMID: 28749594) Brain. 2001 Nov;124(Pt 11):2299-309. (PMID: 11673330) J Exp Med. 2017 Mar 6;214(3):681-697. (PMID: 28183733) Epilepsia. 2006 Sep;47(9):1543-9. (PMID: 16981871) Epilepsia. 2009 Dec;50(12):2648-51. (PMID: 19624715) Neurobiol Dis. 2012 Jan;45(1):369-80. (PMID: 21907282) Neurology. 2012 Feb 21;78(8):526-31. (PMID: 22262746) AJNR Am J Neuroradiol. 2007 Oct;28(9):1662-7. (PMID: 17893226) J Neurosci. 2008 May 21;28(21):5422-32. (PMID: 18495876) Ann Neurol. 2016 Jan;79(1):42-58. (PMID: 26448158) Cereb Cortex. 2018 Oct 1;28(10):3665-3672. (PMID: 29939236) Ann Clin Transl Neurol. 2015 Oct 27;2(12):1041-54. (PMID: 26734657) Neuroimage. 2018 Nov 15;182:511-521. (PMID: 28025129) Epilepsia. 2014 Apr;55(4):539-50. (PMID: 24512506) Epilepsia. 2013 May;54(5):898-908. (PMID: 23551043) Semin Pediatr Neurol. 2006 Mar;13(1):37-42. (PMID: 16818174) Pediatr Neurol. 2013 Oct;49(4):243-54. (PMID: 24053982) Acad Radiol. 2012 Jan;19(1):17-25. (PMID: 22142677) Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772) Brain. 2011 Oct;134(Pt 10):2887-97. (PMID: 21975586) Epilepsia. 2005 Oct;46(10):1646-53. (PMID: 16190938) Acta Neuropathol. 2012 May;123(5):685-93. (PMID: 22327361) PLoS One. 2016 Jun 13;11(6):e0157396. (PMID: 27295297) Neurology. 2015 Nov 3;85(18):1536-45. (PMID: 26432846) Epilepsia. 2008;49 Suppl 2:53-62. (PMID: 18226172) Pediatr Neurol. 2019 Jan;90:24-30. (PMID: 30424962) Glia. 2012 Aug;60(8):1244-50. (PMID: 22438024) Neurology. 2015 Dec 8;85(23):2011-5. (PMID: 26546629) J Neurosci. 2007 May 23;27(21):5546-58. (PMID: 17522300) Brain Pathol. 2016 May;26(3):319-33. (PMID: 26268959) Acta Neuropathol. 2012 Feb;123(2):259-72. (PMID: 22120580) Arch Neurol. 2003 Nov;60(11):1580-4. (PMID: 14623730) IEEE Trans Med Imaging. 2014 Feb;33(2):504-17. (PMID: 24235301) J Neurosci Methods. 2012 Mar 30;205(1):182-9. (PMID: 22227441) Neuroimage. 2011 Jul 1;57(1):167-181. (PMID: 21473920) Neuroimage. 2007 Aug 1;37(1):48-55. (PMID: 17555988) Neuroimage. 2012 Feb 1;59(3):2284-97. (PMID: 22008372) Neuropathol Appl Neurobiol. 2006 Jun;32(3):260-70. (PMID: 16640644) Ann Neurol. 2008 Apr;63(4):454-65. (PMID: 18350576) Anal Quant Cytol Histol. 2001 Aug;23(4):291-9. (PMID: 11531144) Epilepsia. 2009 Oct;50 Suppl 9:9-18. (PMID: 19761449) Acta Neuropathol Commun. 2015 Apr 02;3:17. (PMID: 25853525) Neuroradiology. 2009 Nov;51(11):781-6. (PMID: 19603155) |
معلومات مُعتمدة: | U01 NS082320 United States NS NINDS NIH HHS; International NICHD; International NIMH; W81XWH-11-1-0365 International U.S. Department of Defense; U54 NS092090 United States NS NINDS NIH HHS; U54 HD090255 United States NS NINDS NIH HHS; P30 NS072030 United States NS NINDS NIH HHS; U54 HD090255 United States HD NICHD NIH HHS; UL1 TR001102 United States TR NCATS NIH HHS; R01 NS079788 United States NS NINDS NIH HHS; UL1 TR001102 United States NS NINDS NIH HHS; International NCATS |
تواريخ الأحداث: | Date Created: 20190730 Date Completed: 20200513 Latest Revision: 20200513 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC6649396 |
DOI: | 10.1002/acn3.793 |
PMID: | 31353853 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2328-9503 |
---|---|
DOI: | 10.1002/acn3.793 |