دورية أكاديمية
أعرض في EDS

Phytoscreening of Vochysiaceae species: Molecular identification by HPLC-ESI-MS/MS and evaluating of their antioxidant activity and inhibitory potential against human α-amylase and protein glycation.

التفاصيل البيبلوغرافية
العنوان: Phytoscreening of Vochysiaceae species: Molecular identification by HPLC-ESI-MS/MS and evaluating of their antioxidant activity and inhibitory potential against human α-amylase and protein glycation.
المؤلفون: Franco RR; Instituto de Biotecnologia (IBTEC), Universidade Federal de Uberlândia (UFU), Uberlândia, MG, Brazil., Justino AB; Instituto de Biotecnologia (IBTEC), Universidade Federal de Uberlândia (UFU), Uberlândia, MG, Brazil., Martins MM; Instituto de Biotecnologia (IBTEC), Universidade Federal de Uberlândia (UFU), Uberlândia, MG, Brazil., Silva CG; Serviço de Fitoquímica e Prospecção Farmacêutica, Fundação Ezequiel Dias, Belo Horizonte, 30510-010, MG, Brazil., Campana PRV; Serviço de Fitoquímica e Prospecção Farmacêutica, Fundação Ezequiel Dias, Belo Horizonte, 30510-010, MG, Brazil., Lopes JCD; Departamento de Química, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil., De Almeida VL; Serviço de Fitoquímica e Prospecção Farmacêutica, Fundação Ezequiel Dias, Belo Horizonte, 30510-010, MG, Brazil., Espindola FS; Instituto de Biotecnologia (IBTEC), Universidade Federal de Uberlândia (UFU), Uberlândia, MG, Brazil. Electronic address: foued@ufu.br.
المصدر: Bioorganic chemistry [Bioorg Chem] 2019 Oct; Vol. 91, pp. 103122. Date of Electronic Publication: 2019 Jul 12.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 1303703 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2120 (Electronic) Linking ISSN: 00452068 NLM ISO Abbreviation: Bioorg Chem Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: New York, London, Academic Press.
مواضيع طبية MeSH: Antioxidants/*chemistry , Glycation End Products, Advanced/*antagonists & inhibitors , Glycoside Hydrolase Inhibitors/*chemistry , Hypoglycemic Agents/*chemistry , Myrtales/*chemistry , Phytochemicals/*chemistry, Antioxidants/isolation & purification ; Drug Evaluation, Preclinical ; Enzyme Assays ; Glycoside Hydrolase Inhibitors/isolation & purification ; Humans ; Hypoglycemic Agents/isolation & purification ; Lipase/antagonists & inhibitors ; Phytochemicals/isolation & purification ; Plant Bark/chemistry ; Plant Leaves/chemistry ; alpha-Amylases/antagonists & inhibitors
مستخلص: Scientific research based on medicinal plants has been highlighted as a complementary treatment to T2DM, stand out the Vochysiaceae family, which have been widely used in folk medicine by traditional South American communities to treat some diseases. Our study aimed to investigate the antioxidant and antiglycation activities of ethanol extracts of leaves (LF) and stem barks (SB) of Vochysiaceae species, evaluated their capacities to inhibit glycoside and lipid hydrolases related to T2DM and molecular identification by HPLC-ESI-MS/MS. Our main findings indicate that the ethanolic extract of four of eight analyzed plants such as LF and SB of Q. grandiflora, Q. parviflora, V. elliptica and Calisthene major exhibited, respectively, potential of α-amylase inhibition (IC 50 of LF: 5.7 ± 0.6, 4.1 ± 0.5, 5.8 ± 0.5, 3.2 ± 0.6 and IC 50 of SB: 3.3 ± 0.7, 6.2 ± 2.0, 121.0 ± 8.6 and 11.2 ± 2.8 μg/mL), capacities of antioxidant (ORAC of LF: 516.2 ± 0.1, 547.6 ± 4.9, 544.3 ± 6.1, 442.6 ± 2.4 and ORAC of SB: 593.6 ± 22.3, 497.7 ± 0.8, 578 ± 12.3, 593.6 ± 19.5 µmol trolox eq/g; FRAP of LF: 796.1 ± 0.9, 427.7 ± 22.0, 81.0 ± 1.9, 685 ± 37.9 and FRAP of SB: 947.4 ± 24.9, 738.6 ± 24.3, 98.8 ± 7.9, 970.8 ± 13.9 µmol trolox eq/g; DPPH IC 50 of LF: 14.2 ± 1.8, 36.3 ± 6.9, 11.8 ± 1.9, 13.3 ± 1.2 and DPPH IC 50 of SB: 16.0 ± 3.0, 15.5 ± 1.9, 126.1 ± 23. 6, 5.3 ± 0.3 μg/mL, respectively) and antiglycation (BSA/Frutose IC 50 of LF: 43.1 ± 3.4, 52.1 ± 6.0, 175.5 ± 32, 8, 111.8 ± 14.7 and BSA/Frutose IC 50 of SB:, 40.1 ± 11.9, 51.2 ± 16. 7, 46.6 ± 5.7, 53.5 ± 13.6 μg/mL) and presence of polyphenols, such as flavonoids and condensed tannins. The extracts presented low ability to inhibit α-glycosidase and lipase enzymes in the initial assays, with values below 40% of inhibition. In BSA/methylglyoxal, only Q. grandiflora SB, V. eliptica LF and V. tucanorum LF showed activity (IC 50 : 655.5 ± 208.5, 401.9 ± 135.2 and 617.1 ± 80.6 μg/mL, respectively) and only C. major LF and SB, in Arg/methylglyoxal (IC 50 : 485.1 ± 130.8 and 468.0 ± 150.5 μg/ml, respectively). This study presented new findings about the biological and pharmacological potential of some species of Vochysiaceae family, contributing to the understanding of the action and efficacy in use of these plants, in their management of postprandial hyperglycemia and in glycation and oxidative processes that contribute to managing diabetes mellitus.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Advanced glycation end products; Plant antioxidants; Polyphenols; Postprandial hyperglycemia
المشرفين على المادة: 0 (Antioxidants)
0 (Glycation End Products, Advanced)
0 (Glycoside Hydrolase Inhibitors)
0 (Hypoglycemic Agents)
0 (Phytochemicals)
EC 3.1.1.3 (Lipase)
EC 3.2.1.1 (alpha-Amylases)
تواريخ الأحداث: Date Created: 20190730 Date Completed: 20200929 Latest Revision: 20200929
رمز التحديث: 20231215
DOI: 10.1016/j.bioorg.2019.103122
PMID: 31357074
قاعدة البيانات: MEDLINE
الوصف
تدمد:1090-2120
DOI:10.1016/j.bioorg.2019.103122