دورية أكاديمية
Renal Alterations Induced by the Venom of Colombian Scorpion Centruroides Margaritatus.
| العنوان: | Renal Alterations Induced by the Venom of Colombian Scorpion Centruroides Margaritatus. |
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| المؤلفون: | Galíndez-Cerón JD; Departamento de Biología, Facultad de Ciencias Naturales, Exactas y de la Educación, Grupo de Investigaciones Herpetológicas y Toxinológicas, Universidad del Cauca, Popayán, Colombia., Jorge RJB; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Chavez-Acosta MH; Departamento de Biología, Facultad de Ciencias Naturales, Exactas y de la Educación, Grupo de Investigaciones Herpetológicas y Toxinológicas, Universidad del Cauca, Popayán, Colombia., Jorge ARC; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Alves NTQ; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Prata MMG; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Rodrigues FAP; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Havt A; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Sampaio TL; Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Martins AMC; Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Guerrero-Vargas JA; Departamento de Biología, Facultad de Ciencias Naturales, Exactas y de la Educación, Grupo de Investigaciones Herpetológicas y Toxinológicas, Universidad del Cauca, Popayán, Colombia., Monteiro HSA; Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Beltrán-Vidal JT; Departamento de Biología, Facultad de Ciencias Naturales, Exactas y de la Educación, Grupo de Investigaciones Herpetológicas y Toxinológicas, Universidad del Cauca, Popayán, Colombia. |
| المصدر: | Current topics in medicinal chemistry [Curr Top Med Chem] 2019; Vol. 19 (22), pp. 2049-2057. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bentham Science Publishers Country of Publication: United Arab Emirates NLM ID: 101119673 Publication Model: Print Cited Medium: Internet ISSN: 1873-4294 (Electronic) Linking ISSN: 15680266 NLM ISO Abbreviation: Curr Top Med Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Saif Zone, Sharjah, U.A.E. : Bentham Science Publishers Original Publication: Hilversum, The Netherlands : Bentham Science Publishers, c2001- |
| مواضيع طبية MeSH: | Kidney/*drug effects , Scorpion Venoms/*pharmacology, Animals ; Apoptosis/drug effects ; Cell Survival/drug effects ; Cells, Cultured ; Colombia ; Dogs ; Dose-Response Relationship, Drug ; Kidney/pathology ; Madin Darby Canine Kidney Cells/drug effects ; Madin Darby Canine Kidney Cells/pathology ; Male ; Rats ; Rats, Wistar ; Scorpions ; Structure-Activity Relationship |
| مستخلص: | Background: Scorpion venom causes renal injury and affects vascular ion-channels function. Centruroides margaritatus scorpion is found in Colombia and is frequently the cause of envenomation accidents; however, its renal impact has never been investigated. Objective: To evaluate the effects of C. margaritatus venom (CmV) on renal parameters using isolated rat kidney and renal cell culture models. Methods: Wistar rats (n = 5, weighing 240-300 g) were first perfused with Krebs-Henseleit solution containing 6 g 100 mL-1 bovine serum albumin. After 30 minutes, the kidneys were perfused with CmV to a final concentration of 10 μgmL-1; evaluation was performed by measuring Perfusion Pressure (PP), Renal Vascular Resistance (RVR), Urinary Flow (UF), Glomerular Filtration Rate (GFR), and percentage of electrolyte tubular transport. Moreover, kidney histological analyses and cell cytotoxicity in renal tubule epithelial cells (MDCK) and proximal tubular cells (LLC-MK2) were assessed. Results: CmV increased PP and RVR 60 min after perfusion. On the other hand, UF, GFR, and the percentages of sodium, potassium and chloride tubular transport decreased after experimental envenomation. UF dropped after 120 min, while GFR and percentage of electrolyte tubular transport diminished after 60, 90 and 120 min. CmV was not toxic to MDCK cell line but reduced the viability of LLC-MK2 cells at concentrations ranging from 6.25 to 200 μgmL-1. Histological analyses disclosed hydropic degeneration, edema, and protein deposits. Flow cytometry disclosed that cell death occurred predominantly by necrosis. Conclusion: Our results suggest that C. margaritatus venom can trigger renal impairment, mainly in the proximal kidney tubule. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.) |
| فهرسة مساهمة: | Keywords: Acute kidney injury; Centruroides margaritatus; Cytotoxicity; Kidney perfusion; Necrosis; Scorpion envenomation. |
| المشرفين على المادة: | 0 (Scorpion Venoms) |
| تواريخ الأحداث: | Date Created: 20190801 Date Completed: 20191223 Latest Revision: 20200108 |
| رمز التحديث: | 20231215 |
| DOI: | 10.2174/1568026619666190731143523 |
| PMID: | 31364515 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1873-4294 |
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| DOI: | 10.2174/1568026619666190731143523 |