دورية أكاديمية
Kaempferol Improves TRAIL-Mediated Apoptosis in Leukemia MOLT-4 Cells by the Inhibition of Anti-apoptotic Proteins and Promotion of Death Receptors Expression.
| العنوان: | Kaempferol Improves TRAIL-Mediated Apoptosis in Leukemia MOLT-4 Cells by the Inhibition of Anti-apoptotic Proteins and Promotion of Death Receptors Expression. |
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| المؤلفون: | Hassanzadeh A; Immunology research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Naimi A; Immunology research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Hagh MF; Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Saraei R; Immunology research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Marofi F; Immunology research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Solali S; Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. |
| المصدر: | Anti-cancer agents in medicinal chemistry [Anticancer Agents Med Chem] 2019; Vol. 19 (15), pp. 1835-1845. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bentham Science Publishers Country of Publication: Netherlands NLM ID: 101265649 Publication Model: Print Cited Medium: Internet ISSN: 1875-5992 (Electronic) Linking ISSN: 18715206 NLM ISO Abbreviation: Anticancer Agents Med Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Bentham Science Publishers Original Publication: Sharjah, U.A.E. ; San Francisco, CA : Bentham Science Publishers, [2006]- |
| مواضيع طبية MeSH: | Antineoplastic Agents/*pharmacology , Apoptosis Regulatory Proteins/*antagonists & inhibitors , Kaempferols/*pharmacology , Leukemia/*drug therapy , Receptors, Death Domain/*genetics , TNF-Related Apoptosis-Inducing Ligand/*pharmacology, Apoptosis/drug effects ; Cell Line, Tumor ; Drug Resistance, Neoplasm ; Drug Therapy, Combination ; Gene Expression Regulation, Neoplastic ; Humans ; Intercellular Signaling Peptides and Proteins/genetics ; Intercellular Signaling Peptides and Proteins/metabolism ; Receptors, Death Domain/metabolism ; Receptors, TNF-Related Apoptosis-Inducing Ligand/metabolism ; Time Factors |
| مستخلص: | Introduction: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL or Apo2L) is a member of the Tumor Necrosis Factor (TNF) superfamily, which stimulates apoptosis in a wide range of cancer cells through binding to Death Receptors 4 and 5 (DR4/5). Nevertheless, TRAIL has noticeable anti-cancer abilities; some cancer cells acquire resistance to TRAIL, and consequently, its potential for inducing apoptosis in target cells is strongly diminished. Acute lymphoblastic leukemia MOLT-4 cell line is one of the most resistant cells to TRAIL that developed resistance to TRAIL through different pathways. TRAIL plus kaempferol was used to eliminate the resistance of the MOLT-4 cells to TRAIL. Materials and Methods: Firstly, IC50 for kaempferol (95μM) was determined by using the MTT assay. Secondly, the viability of the MOLT-4 cells was assayed by FACS after Annexin V/PI staining, following treatment with TRAIL (50 and 100nM) and kaempferol (95μM) alone and in combination. Finally, the expression levels of the candidate genes involved in resistance to TRAIL were assayed by real-time PCR technique. Results: Kaempferol plus TRAIL induced apoptosis robustly in MOLT-4 cells at 12, 24 and 48 hours after treatment. Additionally, it was found that kaempferol could inhibit the expression of c-FLIP, X-IAP, cIAP1/2, FGF-8 and VEGF-beta, and conversely augment the expression of DR4/5 in MOLT-4 cells. Conclusion: It is suggested that co-treatment of MOLT-4 cells with TRAIL plus kaempferol is a practical and attractive approach to eliminate cancers' resistance to TRAIL by inhibition of the intracellular anti-apoptotic proteins, upregulation of DR4/5 and also by suppression of the VEGF-beta (VEGFB) and FGF-8 expressions. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.) |
| فهرسة مساهمة: | Keywords: Acute Lymphoblastic Leukemia (ALL); MOLT-4; TRAIL; VEGF; kaempferol; resistance. |
| المشرفين على المادة: | 0 (Antineoplastic Agents) 0 (Apoptosis Regulatory Proteins) 0 (Intercellular Signaling Peptides and Proteins) 0 (Kaempferols) 0 (Receptors, Death Domain) 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand) 0 (TNF-Related Apoptosis-Inducing Ligand) 731P2LE49E (kaempferol) |
| تواريخ الأحداث: | Date Created: 20190801 Date Completed: 20200813 Latest Revision: 20200813 |
| رمز التحديث: | 20221213 |
| DOI: | 10.2174/1871520619666190731155859 |
| PMID: | 31364517 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1875-5992 |
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| DOI: | 10.2174/1871520619666190731155859 |