دورية أكاديمية
The Osteocyte Transcriptome Is Extensively Dysregulated in Mouse Models of Osteogenesis Imperfecta.
العنوان: | The Osteocyte Transcriptome Is Extensively Dysregulated in Mouse Models of Osteogenesis Imperfecta. |
---|---|
المؤلفون: | Zimmerman SM; Department of Physiology and Biophysics University of Arkansas for Medical Sciences Little Rock AR USA., Dimori M; Department of Physiology and Biophysics University of Arkansas for Medical Sciences Little Rock AR USA., Heard-Lipsmeyer ME; Department of Physiology and Biophysics University of Arkansas for Medical Sciences Little Rock AR USA., Morello R; Department of Physiology and Biophysics University of Arkansas for Medical Sciences Little Rock AR USA.; Department of Orthopaedic Surgery University of Arkansas for Medical Sciences Little Rock AR USA.; Division of Genetics University of Arkansas for Medical Sciences Little Rock AR USA. |
المصدر: | JBMR plus [JBMR Plus] 2019 Feb 11; Vol. 3 (7), pp. e10171. Date of Electronic Publication: 2019 Feb 11 (Print Publication: 2019). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 101707013 Publication Model: eCollection Cited Medium: Internet ISSN: 2473-4039 (Electronic) Linking ISSN: 24734039 NLM ISO Abbreviation: JBMR Plus Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Publication: 2024- : Oxford : Oxford University Press Original Publication: [Chichester, West Sussex, UK] : John Wiley & Sons, [2017]- |
مستخلص: | Osteocytes are long-lived, highly interconnected, terminally differentiated osteoblasts that reside within mineralized bone matrix. They constitute about 95% of adult bone cells and play important functions including in the regulation of bone remodeling, phosphate homeostasis, and mechanical stimuli sensing and response. However, the role of osteocytes in the pathogenesis of congenital diseases of abnormal bone matrix is poorly understood. This study characterized in vivo transcriptional changes in osteocytes from Crtap KO and oim/oim mouse models of osteogenesis imperfecta (OI) compared with wild-type (WT) control mice. To do this, RNA was extracted from osteocyte-enriched cortical femurs and tibias, sequenced and subsequently analyzed to identify differentially expressed transcripts. These models were chosen because they mimic two types of OI with different genetic mutations that result in distinct type I collagen defects. A large number of transcripts were dysregulated in either model of OI, but 281 of them were similarly up- or downregulated in both compared with WT controls. Conversely, very few transcripts were differentially expressed between the Crtap KO and oim/oim mice, indicating that distinct alterations in type I collagen can lead to shared pathogenic processes and similar phenotypic outcomes. Bioinformatics analyses identified several critical hubs of dysregulation that were enriched in annotation terms such as development and differentiation, ECM and collagen fibril organization, cell adhesion, signaling, regulatory processes, pattern binding, chemotaxis, and cell projections. The data further indicated alterations in important signaling pathways such as WNT and TGF-β but also highlighted new candidate genes to pursue in future studies. Overall, our study suggested that the osteocyte transcriptome is broadly dysregulated in OI with potential long-term consequences at the cellular level, which deserve further investigations. © 2019 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research. |
References: | Development. 1999 Oct;126(19):4267-79. (PMID: 10477295) Bone. 2000 Jun;26(6):581-9. (PMID: 10831929) J Cell Biol. 2000 Sep 18;150(6):1499-506. (PMID: 10995453) J Pathol. 2000 Nov;192(3):385-95. (PMID: 11054723) J Cell Biochem. 2001;81(1):82-92. (PMID: 11180399) EMBO J. 2001 May 1;20(9):2254-72. (PMID: 11331591) J Bone Miner Res. 2001 Oct;16(10):1754-64. (PMID: 11585338) J Bone Miner Res. 2002 Jun;17(6):1015-25. (PMID: 12054156) J Biol Chem. 2002 Nov 15;277(46):44061-7. (PMID: 12226090) Mol Endocrinol. 2004 May;18(5):1222-37. (PMID: 14976225) J Biol Chem. 2004 Dec 17;279(51):53331-7. (PMID: 15383546) Bioinformatics. 2005 Aug 15;21(16):3448-9. (PMID: 15972284) Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W741-8. (PMID: 15980575) EMBO J. 2005 Jul 20;24(14):2543-55. (PMID: 15990875) Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11527-32. (PMID: 16051705) FASEB J. 2005 Nov;19(13):1842-4. (PMID: 16123173) Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50. (PMID: 16199517) Bone. 2006 Oct;39(4):754-66. (PMID: 16730481) Cell. 2006 Oct 20;127(2):291-304. (PMID: 17055431) Eur J Histochem. 2007;51 Suppl 1:15-9. (PMID: 17703589) Genome Biol. 2007;8(9):R183. (PMID: 17784955) Biochim Biophys Acta. 2008 Mar;1777(3):260-8. (PMID: 18194661) Clin J Am Soc Nephrol. 2008 Nov;3 Suppl 3:S131-9. (PMID: 18988698) Cells Tissues Organs. 2009;190(2):69-80. (PMID: 19033678) Nat Protoc. 2009;4(1):44-57. (PMID: 19131956) Genome Biol. 2009;10(3):R25. (PMID: 19261174) Bioinformatics. 2009 May 1;25(9):1105-11. (PMID: 19289445) Bone. 2009 Oct;45(4):682-92. (PMID: 19539797) Trends Endocrinol Metab. 2010 May;21(5):294-301. (PMID: 20079660) Am J Hum Genet. 2010 Mar 12;86(3):389-98. (PMID: 20188343) Trends Endocrinol Metab. 2010 Jun;21(6):369-74. (PMID: 20223679) Nat Biotechnol. 2010 May;28(5):511-5. (PMID: 20436464) Proteomics. 2010 Oct;10(20):3688-98. (PMID: 20845334) J Cell Biol. 2011 Mar 21;192(6):1057-72. (PMID: 21402791) Nat Med. 2011 Sep 11;17(10):1235-41. (PMID: 21909103) Nat Med. 2011 Sep 11;17(10):1231-4. (PMID: 21909105) Clin Biochem. 2012 Aug;45(12):863-73. (PMID: 22465238) J Proteomics. 2012 Aug 3;75(15):4717-33. (PMID: 22846432) Bone. 2013 Jun;54(2):258-63. (PMID: 22939943) J Med Genet. 2013 May;50(5):345-8. (PMID: 23434763) Bone. 2013 Aug;55(2):465-75. (PMID: 23598046) Endocr Rev. 2013 Oct;34(5):658-90. (PMID: 23612223) N Engl J Med. 2013 May 9;368(19):1809-16. (PMID: 23656646) Calcif Tissue Int. 2014 Jan;94(1):46-54. (PMID: 23657489) Nucleic Acids Res. 2013 Jul;41(Web Server issue):W77-83. (PMID: 23703215) Calcif Tissue Int. 2014 Jan;94(1):5-24. (PMID: 24042263) Bonekey Rep. 2014 Jan 08;3:481. (PMID: 24466412) Hum Mol Genet. 2014 Aug 1;23(15):4035-42. (PMID: 24634143) J Cell Biochem. 2014 Aug;115(8):1420-9. (PMID: 24700678) Nat Med. 2014 Jun;20(6):670-5. (PMID: 24793237) Mol Endocrinol. 2014 Jul;28(7):1150-65. (PMID: 24877565) Bone. 2014 Sep;66:146-54. (PMID: 24933342) J Bone Miner Res. 2015 Aug;30(8):1457-67. (PMID: 25678055) Matrix Biol. 2015 May-Jul;44-46:46-53. (PMID: 25863161) Hum Mol Genet. 2015 Nov 1;24(21):6118-33. (PMID: 26264579) Methods Mol Biol. 2016;1344:287-300. (PMID: 26520132) Lancet. 2016 Apr 16;387(10028):1657-71. (PMID: 26542481) J Bone Miner Res. 2016 Aug;31(8):1608-1616. (PMID: 26925839) Nat Rev Endocrinol. 2016 Oct;12(10):593-605. (PMID: 27230951) Am J Physiol Endocrinol Metab. 2016 Sep 1;311(3):E587-93. (PMID: 27460899) J Biol Chem. 2016 Nov 25;291(48):24838-24850. (PMID: 27733688) J Cell Physiol. 2017 Nov;232(11):3006-3019. (PMID: 28488815) J Clin Invest. 2017 Jun 30;127(7):2678-2688. (PMID: 28628032) J Proteomics. 2017 Sep 7;167:46-59. (PMID: 28802583) Nat Rev Dis Primers. 2017 Aug 18;3:17052. (PMID: 28820180) Tumour Biol. 2017 Oct;39(10):1010428317712166. (PMID: 29022485) Sci Signal. 2017 Nov 21;10(506):null. (PMID: 29162742) Bone Rep. 2018 Jul 02;9:61-73. (PMID: 30105276) Proc Natl Acad Sci U S A. 1988 Mar;85(5):1492-6. (PMID: 3422746) Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1701-5. (PMID: 8446583) J Cell Biol. 1996 Jan;132(1-2):195-210. (PMID: 8567723) Ital J Anat Embryol. 1996 Oct-Dec;101(4):25-79. (PMID: 9203871) Bone. 1998 Mar;22(3):181-7. (PMID: 9514209) |
معلومات مُعتمدة: | P20 GM125503 United States GM NIGMS NIH HHS; R01 AR060823 United States AR NIAMS NIH HHS |
فهرسة مساهمة: | Keywords: COLLAGEN; GENETIC ANIMAL MODELS; OSTEOCYTES; OSTEOGENESIS IMPERFECTA; RNASEQ |
تواريخ الأحداث: | Date Created: 20190803 Latest Revision: 20201001 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC6659450 |
DOI: | 10.1002/jbm4.10171 |
PMID: | 31372585 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2473-4039 |
---|---|
DOI: | 10.1002/jbm4.10171 |