دورية أكاديمية
Transcriptome analyses of cells carrying the Type II Csp231I restriction-modification system reveal cross-talk between two unrelated transcription factors: C protein and the Rac prophage repressor.
العنوان: | Transcriptome analyses of cells carrying the Type II Csp231I restriction-modification system reveal cross-talk between two unrelated transcription factors: C protein and the Rac prophage repressor. |
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المؤلفون: | Negri A; Department of Microbiology, Faculty of Biology, University of Gdansk, Wita Stwosza 59, Gdansk 80-308, Poland., Jąkalski M; Department of Plant Taxonomy and Nature Conservation, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland., Szczuka A; Department of Microbiology, Faculty of Biology, University of Gdansk, Wita Stwosza 59, Gdansk 80-308, Poland., Pryszcz LP; Laboratory of Zebrafish Developmental Genomics, International Institute of Molecular and Cell Biology, Warsaw, ul. Trojdena 4, 02-109 Warsaw, Poland., Mruk I; Department of Microbiology, Faculty of Biology, University of Gdansk, Wita Stwosza 59, Gdansk 80-308, Poland. |
المصدر: | Nucleic acids research [Nucleic Acids Res] 2019 Oct 10; Vol. 47 (18), pp. 9542-9556. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 1992- : Oxford : Oxford University Press Original Publication: London, Information Retrieval ltd. |
مواضيع طبية MeSH: | Bacteriophages/*genetics , DNA Restriction-Modification Enzymes/*genetics , Escherichia coli/*genetics , Host-Pathogen Interactions/*genetics, Amino Acid Sequence/genetics ; Bacteriophages/pathogenicity ; Citrobacter/genetics ; DNA Restriction Enzymes/genetics ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Deoxyribonucleases, Type II Site-Specific/genetics ; Escherichia coli/virology ; Gene Expression Profiling/methods ; Gene Expression Regulation, Bacterial ; Gene Transfer, Horizontal/genetics ; Phenotype ; Transcription Factors/genetics ; Viral Proteins/genetics |
مستخلص: | Restriction-modification (R-M) systems represent an effective mechanism of defence against invading bacteriophages, and are widely spread among bacteria and archaea. In acquiring a Type II R-M system via horizontal gene transfer, the new hosts become more resistant to phage infection, through the action of a restriction endonuclease (REase), which recognizes and cleaves specific target DNAs. To protect the host cell's DNA, there is also a methyltransferase (MTase), which prevents DNA cleavage by the cognate REase. In some R-M systems, the host also accepts a cis-acting transcription factor (C protein), which regulates the counteracting activities of REase and MTase to avoid host self-restriction. Our study characterized the unexpected phenotype of Escherichia coli cells, which manifested as extensive cell filamentation triggered by acquiring the Csp231I R-M system from Citrobacter sp. Surprisingly, we found that the cell morphology defect was solely dependent on the C regulator. Our transcriptome analysis supported by in vivo and in vitro assays showed that C protein directly silenced the expression of the RacR repressor to affect the Rac prophage-related genes. The rac locus ydaST genes, when derepressed, exerted a toxicity indicated by cell filamentation through an unknown mechanism. These results provide an apparent example of transcription factor cross-talk, which can have significant consequences for the host, and may represent a constraint on lateral gene transfer. (© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.) |
References: | Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep;69(Pt 9):962-6. (PMID: 23989141) Acta Crystallogr D Biol Crystallogr. 2015 Feb;71(Pt 2):398-407. (PMID: 25664751) Biochimie. 1999 Aug-Sep;81(8-9):931-8. (PMID: 10572308) MBio. 2017 Feb 21;8(1):. (PMID: 28223461) Mol Biol Evol. 2008 Mar;25(3):559-67. (PMID: 18158322) Nucleic Acids Res. 2014;42(16):10618-31. (PMID: 25120263) Nucleic Acids Res. 2017 Aug 21;45(14):8423-8434. (PMID: 28854737) Genetics. 2014 Nov;198(3):1155-66. (PMID: 25173845) PLoS One. 2016 Feb 05;11(2):e0148355. (PMID: 26848973) Curr Biol. 2016 Feb 8;26(3):404-9. (PMID: 26804559) J Bacteriol. 2000 Apr;182(8):2218-29. (PMID: 10735865) Proc Natl Acad Sci U S A. 1998 May 26;95(11):6442-7. (PMID: 9600985) Trends Genet. 2009 Oct;25(10):434-40. (PMID: 19815308) Science. 2007 Nov 30;318(5855):1449-52. (PMID: 17947550) Sci Rep. 2018 Apr 3;8(1):5550. (PMID: 29615803) mSphere. 2018 Apr 25;3(2):. (PMID: 29695625) Curr Biol. 2015 Aug 3;25(15):2034-9. (PMID: 26190075) Nat Commun. 2015 Apr 21;6:6845. (PMID: 25897488) Gene. 2006 Sep 1;379:109-15. (PMID: 16750601) Nucleic Acids Res. 2012 Nov 1;40(20):10532-42. (PMID: 22941636) BMC Evol Biol. 2008 Jan 24;8:23. (PMID: 18218112) Gastroenterology. 2018 Feb;154(3):612-623.e7. (PMID: 29066327) Sci Am. 1982 Nov;247(5):128-30, 132, 134-40. (PMID: 6216590) J Bacteriol. 2000 Jan;182(2):477-87. (PMID: 10629196) J Bacteriol. 1995 Nov;177(21):6313-5. (PMID: 7592403) Virology. 2003 Sep 1;313(2):622-8. (PMID: 12954227) Mol Microbiol. 2018 Oct;110(1):1-10. (PMID: 29995343) Nucleic Acids Res. 2008 Mar;36(5):1429-42. (PMID: 18203750) PLoS One. 2017 Sep 21;12(9):e0185234. (PMID: 28934361) PLoS Pathog. 2013;9(7):e1003419. (PMID: 23853579) J Bacteriol. 2018 Nov 26;200(24):. (PMID: 30249703) Proc Natl Acad Sci U S A. 2016 May 17;113(20):5658-63. (PMID: 27140615) J Bacteriol. 2011 Nov;193(21):6039-56. (PMID: 21908672) Nucleic Acids Res. 2008 May;36(8):2581-93. (PMID: 18334533) Microbiol Mol Biol Rev. 2009 Sep;73(3):481-509, Table of Contents. (PMID: 19721087) Appl Environ Microbiol. 2007 Jul;73(13):4286-93. (PMID: 17468281) Nucleic Acids Res. 2003 Jan 15;31(2):743-9. (PMID: 12527784) J Mol Biol. 2006 Apr 28;358(2):523-31. (PMID: 16516922) Genes Dev. 2004 Sep 1;18(17):2086-94. (PMID: 15342489) Nucleic Acids Res. 2009 Feb;37(2):441-51. (PMID: 19056824) BMC Microbiol. 2017 Jan 31;17(1):26. (PMID: 28143392) Nucleic Acids Res. 2010 Apr;38(7):2428-43. (PMID: 20071371) mSphere. 2017 Nov 22;2(6):null. (PMID: 29205229) Trends Microbiol. 2018 Aug;26(8):715-726. (PMID: 29452952) Trends Genet. 2014 Jun;30(6):211-9. (PMID: 24774859) Science. 1988 Nov 11;242(4880):899-907. (PMID: 3187531) Nucleic Acids Res. 2007;35(20):6935-52. (PMID: 17933763) PLoS Comput Biol. 2011 Oct;7(10):e1002195. (PMID: 22039361) Trends Microbiol. 2015 Oct;23(10):598-605. (PMID: 26433693) Nucleic Acids Res. 2016 Apr 7;44(6):2646-60. (PMID: 26656489) J Bacteriol. 1999 Nov;181(21):6822-7. (PMID: 10542186) J Mol Biol. 2005 Feb 25;346(3):689-701. (PMID: 15713456) J Bacteriol. 1996 Sep;178(17):5100-4. (PMID: 8752325) J Bacteriol. 1991 Feb;173(4):1367-75. (PMID: 1995588) J Microbiol Biotechnol. 2010 Jan;20(1):30-8. (PMID: 20134230) Nucleic Acids Res. 2001 Sep 15;29(18):3742-56. (PMID: 11557807) Microbiol Mol Biol Rev. 2013 Mar;77(1):53-72. (PMID: 23471617) PLoS Genet. 2011 Jan 27;7(1):e1001284. (PMID: 21298028) Nucleic Acids Res. 2005 Dec 06;33(21):6942-51. (PMID: 16332697) Viruses. 2018 Mar 07;10(3):. (PMID: 29518935) Gut Pathog. 2014 Sep 05;6:35. (PMID: 25349630) Nucleic Acids Res. 2013 Jul;41(12):6045-57. (PMID: 23630319) Acta Crystallogr D Biol Crystallogr. 2009 Sep;65(Pt 9):900-5. (PMID: 19690367) Gene. 2003 Oct 23;317(1-2):13-6. (PMID: 14604787) Nucleic Acids Res. 2018 Nov 16;46(20):10810-10826. (PMID: 30295835) Nucleic Acids Res. 2009 Feb;37(3):983-98. (PMID: 19126580) Nat Commun. 2016 Aug 04;7:12307. (PMID: 27489144) Mol Microbiol. 2003 Jul;49(2):277-300. (PMID: 12886937) Mol Microbiol. 2013 Apr;88(1):203-18. (PMID: 23480529) Nat Commun. 2010;1:147. (PMID: 21266997) Front Biosci (Landmark Ed). 2009 Jan 01;14:4103-12. (PMID: 19273337) mSphere. 2017 Nov 22;2(6):null. (PMID: 29205228) Nucleic Acids Res. 2014 Feb;42(4):2415-32. (PMID: 24302578) Science. 1995 Feb 10;267(5199):897-9. (PMID: 7846533) Nat Rev Microbiol. 2010 May;8(5):317-27. (PMID: 20348932) Nucleic Acids Res. 2006 Jul 13;34(12):3434-45. (PMID: 16840530) Nucleic Acids Res. 2002 Aug 15;30(16):3558-65. (PMID: 12177297) Nat Protoc. 2009;4(2):206-23. (PMID: 19180090) Nat Rev Microbiol. 2005 Sep;3(9):711-21. (PMID: 16138099) Nucleic Acids Res. 2014 Jun;42(10):6448-62. (PMID: 24748661) Nucleic Acids Res. 2016 Jan 29;44(2):790-800. (PMID: 26687717) Nucleic Acids Res. 2015 Jan;43(Database issue):D298-9. (PMID: 25378308) Nucleic Acids Res. 2009 Jun;37(10):3354-66. (PMID: 19336410) Nucleic Acids Res. 2014 Jan;42(1):3-19. (PMID: 24141096) PLoS Genet. 2014 Apr 10;10(4):e1004272. (PMID: 24722038) Mol Syst Biol. 2018 Jun 25;14(6):e7573. (PMID: 29941428) Nucleic Acids Res. 2014 Jan;42(1):70-86. (PMID: 23945938) Biophys J. 2017 Apr 25;112(8):1539-1550. (PMID: 28445746) |
المشرفين على المادة: | 0 (DNA Restriction-Modification Enzymes) 0 (DNA, Bacterial) 0 (Transcription Factors) 0 (Viral Proteins) EC 3.1.21.- (DNA Restriction Enzymes) EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific) |
تواريخ الأحداث: | Date Created: 20190803 Date Completed: 20191203 Latest Revision: 20191203 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC6765115 |
DOI: | 10.1093/nar/gkz665 |
PMID: | 31372643 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1362-4962 |
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DOI: | 10.1093/nar/gkz665 |