دورية أكاديمية
Efficient electron-mediated electrochemical biosensor of gold wire for the rapid detection of C-reactive protein: A predictive strategy for heart failure.
| العنوان: | Efficient electron-mediated electrochemical biosensor of gold wire for the rapid detection of C-reactive protein: A predictive strategy for heart failure. |
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| المؤلفون: | Vilian ATE; Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul, 100-715, Republic of Korea., Kim W; Department of Physics, Inha University, 100 Inha-ro, Michuhol-gu, Incheon, 22212, Republic of Korea., Park B; Department of Biological Engineering, Inha University, 100 Inha-ro, Michuhol-gu, Incheon, 22212, Republic of Korea., Oh SY; Department of Biological Engineering, Inha University, 100 Inha-ro, Michuhol-gu, Incheon, 22212, Republic of Korea., Kim T; Department of Physics, Inha University, 100 Inha-ro, Michuhol-gu, Incheon, 22212, Republic of Korea., Huh YS; Department of Biological Engineering, Inha University, 100 Inha-ro, Michuhol-gu, Incheon, 22212, Republic of Korea. Electronic address: yunsuk.huh@inha.ac.kr., Hwangbo CK; Department of Physics, Inha University, 100 Inha-ro, Michuhol-gu, Incheon, 22212, Republic of Korea. Electronic address: hwangbo@inha.ac.kr., Han YK; Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul, 100-715, Republic of Korea. Electronic address: ykenergy@dongguk.edu. |
| المصدر: | Biosensors & bioelectronics [Biosens Bioelectron] 2019 Oct 01; Vol. 142, pp. 111549. Date of Electronic Publication: 2019 Jul 31. |
| نوع المنشور: | Evaluation Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Advanced Technology Country of Publication: England NLM ID: 9001289 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4235 (Electronic) Linking ISSN: 09565663 NLM ISO Abbreviation: Biosens Bioelectron Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Elsevier Advanced Technology Original Publication: [Barking, Essex, England] : Elsevier Applied Science, 1989- |
| مواضيع طبية MeSH: | Antibodies, Immobilized/*chemistry , Biosensing Techniques/*instrumentation , C-Reactive Protein/*analysis , Gold/*chemistry , Polycarboxylate Cement/*chemistry, Biomarkers/analysis ; Biomarkers/blood ; Biosensing Techniques/methods ; Electrochemical Techniques/instrumentation ; Electrochemical Techniques/methods ; Electrons ; Equipment Design ; Heart Failure/blood ; Heart Failure/diagnosis ; Humans ; Limit of Detection |
| مستخلص: | C-reactive protein (CRP) is considered a promising biomarker for the rapid and high-throughput real-time monitoring of cardiovascular disease and inflammation in unprocessed clinical samples. Implementation of this monitoring would enable various transformative biomedical applications. We have fabricated a highly specific sensor chip to detect CRP with a detection limit of 2.25 fg/mL. The protein was immobilized on top of a gold (Au) wire/polycarbonate (PC) substrate using 1-ethyl-3-(3-dimethylamino-propyl) carbodiimide hydrochloride/N-hydroxy succinimide-activated 3-mercaptoproponic acid (MPA) as a self-assembled monolayer agent and bovine serum albumin (BSA) as a blocking agent. In contrast to the bare PC substrate, the CRP/BSA/anti-CRP/MPA/Au substrate exhibited a considerably high electrochemical signal toward CRP. The influence of the experimental parameters on CRP detection was assessed via various analysis methods, and these parameters were then optimized. The linear dynamic range of the CRP was 5-220 fg/mL for voltammetric and impedance analysis. Morever, the strategy exhibited high selectivity against various potential interfering species and was capable of directly probing trace amounts of the target CRP in human serum with excellent selectivity. The analytical assay based on the CRP/BSA/anti-CRP/MPA/Au substrate could be exploited as a potentially useful tool for detecting CRP in clinical samples. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: C-reactive protein; Electrochemical detection; Gold wires; Immunosensor; Square wave voltammetry |
| المشرفين على المادة: | 0 (Antibodies, Immobilized) 0 (Biomarkers) 0 (Polycarboxylate Cement) 25766-59-0 (polycarbonate) 7440-57-5 (Gold) 9007-41-4 (C-Reactive Protein) |
| تواريخ الأحداث: | Date Created: 20190811 Date Completed: 20200131 Latest Revision: 20200131 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.bios.2019.111549 |
| PMID: | 31400725 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4235 |
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| DOI: | 10.1016/j.bios.2019.111549 |