دورية أكاديمية
P Granules Protect RNA Interference Genes from Silencing by piRNAs.
| العنوان: | P Granules Protect RNA Interference Genes from Silencing by piRNAs. |
|---|---|
| المؤلفون: | Ouyang JPT; HHMI and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Folkmann A; HHMI and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Bernard L; HHMI and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Lee CY; HHMI and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA., Seroussi U; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada., Charlesworth AG; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada., Claycomb JM; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada., Seydoux G; HHMI and Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: gseydoux@jhmi.edu. |
| المصدر: | Developmental cell [Dev Cell] 2019 Sep 23; Vol. 50 (6), pp. 716-728.e6. Date of Electronic Publication: 2019 Aug 08. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101120028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1551 (Electronic) Linking ISSN: 15345807 NLM ISO Abbreviation: Dev Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, Mass. : Cell Press, c2001- |
| مواضيع طبية MeSH: | RNA Interference*, Caenorhabditis elegans/*genetics , Cytoplasmic Granules/*genetics , RNA, Small Interfering/*genetics, Animals ; Caenorhabditis elegans/embryology ; Caenorhabditis elegans Proteins/metabolism ; Cell Nucleus/metabolism ; Embryo, Nonmammalian/metabolism ; Epigenesis, Genetic ; Genes, Helminth ; Genetic Loci ; Germ Cells/metabolism ; Models, Biological ; Mutation/genetics ; Phenotype ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Small Interfering/metabolism ; Transcription, Genetic ; Up-Regulation/genetics |
| مستخلص: | P granules are perinuclear condensates in C. elegans germ cells proposed to serve as hubs for self/non-self RNA discrimination by Argonautes. We report that a mutant (meg-3 meg-4) that does not assemble P granules in primordial germ cells loses competence for RNA-interference over several generations and accumulates silencing small RNAs against hundreds of endogenous genes, including the RNA-interference genes rde-11 and sid-1. In wild type, rde-11 and sid-1 transcripts are heavily targeted by piRNAs and accumulate in P granules but maintain expression. In the primordial germ cells of meg-3 meg-4 mutants, rde-11 and sid-1 transcripts disperse in the cytoplasm with the small RNA biogenesis machinery, become hyper-targeted by secondary sRNAs, and are eventually silenced. Silencing requires the PIWI-class Argonaute PRG-1 and the nuclear Argonaute HRDE-1 that maintains trans-generational silencing of piRNA targets. These observations support a "safe harbor" model for P granules in protecting germline transcripts from piRNA-initiated silencing. (Copyright © 2019 Elsevier Inc. All rights reserved.) |
| التعليقات: | Comment in: Dev Cell. 2019 Sep 23;50(6):677-679. (PMID: 31550461) |
| References: | Development. 1999 Nov;126(21):4861-71. (PMID: 10518502) Mol Cell. 2000 Apr;5(4):671-82. (PMID: 10882103) Science. 2002 Mar 29;295(5564):2456-9. (PMID: 11834782) Nat Rev Genet. 2003 Feb;4(2):112-20. (PMID: 12560808) Science. 2003 Sep 12;301(5639):1545-7. (PMID: 12970568) Dev Cell. 2003 Nov;5(5):747-57. (PMID: 14602075) Science. 2005 May 20;308(5725):1164-7. (PMID: 15790806) Cell. 2006 Nov 17;127(4):747-57. (PMID: 17110334) Science. 2007 Jan 12;315(5809):241-4. (PMID: 17124291) Science. 2007 Jan 12;315(5809):244-7. (PMID: 17158288) Curr Biol. 2008 Jun 24;18(12):861-7. (PMID: 18501605) Mol Cell. 2008 Jul 11;31(1):67-78. (PMID: 18571452) Science. 2008 Jul 25;321(5888):537-41. (PMID: 18653886) Science. 2008 Nov 28;322(5906):1387-92. (PMID: 19039138) Cell. 2009 Jan 23;136(2):308-21. (PMID: 19167332) Bioinformatics. 2009 Aug 15;25(16):2078-9. (PMID: 19505943) Mol Cell. 2009 Oct 23;36(2):231-44. (PMID: 19800275) Cell. 2009 Oct 2;139(1):123-34. (PMID: 19804758) Nat Biotechnol. 2011 Jan;29(1):24-6. (PMID: 21221095) Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1201-8. (PMID: 21245313) Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286) Genes Dev. 2012 Apr 15;26(8):846-56. (PMID: 22508728) Curr Biol. 2012 May 22;22(10):881-90. (PMID: 22542102) Genes Dev. 2012 Jul 1;26(13):1433-44. (PMID: 22713602) Cell. 2012 Jul 6;150(1):78-87. (PMID: 22738724) Cell. 2012 Jul 6;150(1):88-99. (PMID: 22738725) Cell. 2012 Jul 6;150(1):65-77. (PMID: 22738726) Nature. 2012 Sep 20;489(7416):447-51. (PMID: 22810588) Adv Exp Med Biol. 2013;757:17-39. (PMID: 22872473) Mol Cell. 2013 Jun 27;50(6):894-907. (PMID: 23806335) Dev Cell. 2013 Dec 23;27(6):656-63. (PMID: 24360782) Nat Struct Mol Biol. 2014 Apr;21(4):358-65. (PMID: 24681887) Bioinformatics. 2015 Jan 15;31(2):166-9. (PMID: 25260700) Genome Biol. 2014;15(12):550. (PMID: 25516281) Elife. 2014 Dec 23;3:e04591. (PMID: 25535836) Nat Methods. 2015 Apr;12(4):357-60. (PMID: 25751142) Genetics. 2015 Aug;200(4):1035-49. (PMID: 26044593) Dev Cell. 2015 Aug 24;34(4):448-56. (PMID: 26279485) Dev Cell. 2015 Aug 24;34(4):457-65. (PMID: 26279487) Development. 2015 Oct 15;142(20):3571-82. (PMID: 26395476) Cell. 2016 Mar 24;165(1):88-99. (PMID: 27015309) Mol Cell. 2017 Feb 16;65(4):659-670.e5. (PMID: 28212751) Methods. 2017 May 15;121-122:86-93. (PMID: 28392263) Genetics. 2017 Oct;207(2):547-557. (PMID: 28751423) Trends Genet. 2017 Nov;33(11):882-894. (PMID: 28964526) Elife. 2017 Oct 05;6:. (PMID: 28980937) Science. 2018 Feb 2;359(6375):587-592. (PMID: 29420292) Cell. 2018 Feb 22;172(5):937-951.e18. (PMID: 29456082) Nature. 2018 May;557(7707):679-683. (PMID: 29769721) Mol Cell. 2018 May 17;70(4):639-649.e6. (PMID: 29775580) BMC Biol. 2018 Sep 18;16(1):103. (PMID: 30227863) Development. 2019 Mar 25;146(6):null. (PMID: 30814118) Nat Struct Mol Biol. 2019 Mar;26(3):220-226. (PMID: 30833787) Cell. 1998 Sep 4;94(5):635-45. (PMID: 9741628) Development. 1999 Jan;126(1):1-11. (PMID: 9834181) |
| معلومات مُعتمدة: | P30 NS050274 United States NS NINDS NIH HHS; R37 HD037047 United States HD NICHD NIH HHS; S10 OD023548 United States OD NIH HHS; T32 GM007445 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: Argonautes; P granules; RNA-mediated interference; epigenetic silencing; piRNAs |
| المشرفين على المادة: | 0 (Caenorhabditis elegans Proteins) 0 (RNA, Messenger) 0 (RNA, Small Interfering) |
| تواريخ الأحداث: | Date Created: 20190813 Date Completed: 20200518 Latest Revision: 20231107 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC6764750 |
| DOI: | 10.1016/j.devcel.2019.07.026 |
| PMID: | 31402283 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1878-1551 |
|---|---|
| DOI: | 10.1016/j.devcel.2019.07.026 |