دورية أكاديمية
Evaluation of Toxoplasma gondii propagated in specific pathogen free embryonated chicken egg, for diagnosis of toxoplasmosis in equids and human.
| العنوان: | Evaluation of Toxoplasma gondii propagated in specific pathogen free embryonated chicken egg, for diagnosis of toxoplasmosis in equids and human. |
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| المؤلفون: | Abdalhamed AM; 1Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622 Giza, Egypt., Hassanain MA; 2Zoonotic Diseases Department, Veterinary Research Division, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622, Giza, Egypt., Zeedan GSG; 1Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622 Giza, Egypt., Shaapan RM; 2Zoonotic Diseases Department, Veterinary Research Division, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622, Giza, Egypt. |
| المصدر: | Journal of parasitic diseases : official organ of the Indian Society for Parasitology [J Parasit Dis] 2019 Sep; Vol. 43 (3), pp. 498-505. Date of Electronic Publication: 2019 Apr 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer India Country of Publication: India NLM ID: 9713059 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0971-7196 (Print) Linking ISSN: 09717196 NLM ISO Abbreviation: J Parasit Dis Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: <2009-> : New Dehli : Springer India Original Publication: Lucknow, India : Indian Society for Parasitology, c1995- |
| مستخلص: | Toxoplasma gondii ( T. gondii ) is a worldwide distribution infects a wide variety of mammals, including humans. The present study aimed to detect the efficacy of soluble and whole T. gondii antigens propagated in specific pathogen-free of embryonated chicken egg (SPF-ECE) used to improve the potency of serological assays for diagnosis of toxoplasmosis in equids and human. Total of 220 serum samples from 170 equids (90 donkeys and 55 horses and 25 mules) and 50 humans were collected from different governorates in Egypt during the period from October 2017 to March 2018. Crude T. gondii tachyzoites antigens from low or high passages propagated in mice or SPF-ECE was used for modifying some serological tests. The experiment showed that the mortality rate of T. gondii for 10 3 and 10 4 low passages were 6/8 (75%) and 7/8 (88%) dead embryos but, lower mortality rate in high passage T. gondii were 4/8 (50%) and 5/8 (63%) dead embryos, respectively. No mortality or inflammatory signs were observed in control of negative groups. In equids sera were examined by S-ELISA using soluble T. gondii antigen propagated in SPF-ECE showed the highest positive results 26 (28.8%), followed by LAT 37 (22%) and MAGPT 36 (21.17%). While, W-ELISA and IFAT used whole T. gondii antigen prepared in SPF-ECE were 35 (20.58%) and 28 (19.41%) showed highly positive results than the same test used the whole antigen prepared in mice. The highest seroprevalence of T. gondii in human and donkeys were 19/50 (38%). and 26/90 (28.88%), more than mules were 6/25 (24%) and horses were 9/55 (16.3%) examined by S-ELISA respectively. SPF-ECE is considered an appropriate experimental model for isolation and propagation of T. gondii tachyzoites , and their soluble antigens used in serological tests (S-ELISA, LAT, and MAGPT) have sensitivity and specificity more than the whole antigen and provided reliable diagnostic tools for detection of toxoplasmosis in human and equids. Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest. |
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| فهرسة مساهمة: | Keywords: ELISA; Equids; I FAT; LAT; MAGPT; SPF-ECE; T. gondii |
| تواريخ الأحداث: | Date Created: 20190814 Latest Revision: 20200930 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC6667588 |
| DOI: | 10.1007/s12639-019-01117-3 |
| PMID: | 31406416 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 0971-7196 |
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| DOI: | 10.1007/s12639-019-01117-3 |