دورية أكاديمية
أعرض في EDS

The Landscape of Genetic Content in the Gut and Oral Human Microbiome.

التفاصيل البيبلوغرافية
العنوان: The Landscape of Genetic Content in the Gut and Oral Human Microbiome.
المؤلفون: Tierney BT; Section on Pathophysiology and Molecular Pharmacology, Joslin Diabetes Center, Boston, MA, USA; Section on Islet Cell and Regenerative Biology, Joslin Diabetes Center, Boston, MA, USA; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA; Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA., Yang Z; Section on Pathophysiology and Molecular Pharmacology, Joslin Diabetes Center, Boston, MA, USA; Section on Islet Cell and Regenerative Biology, Joslin Diabetes Center, Boston, MA, USA; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA; Department of Combinatorics and Optimization, University of Waterloo, Waterloo, Ontario, Canada., Luber JM; Section on Pathophysiology and Molecular Pharmacology, Joslin Diabetes Center, Boston, MA, USA; Section on Islet Cell and Regenerative Biology, Joslin Diabetes Center, Boston, MA, USA; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA; Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA., Beaudin M; Section on Pathophysiology and Molecular Pharmacology, Joslin Diabetes Center, Boston, MA, USA; Section on Islet Cell and Regenerative Biology, Joslin Diabetes Center, Boston, MA, USA; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA; Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada., Wibowo MC; Section on Pathophysiology and Molecular Pharmacology, Joslin Diabetes Center, Boston, MA, USA; Section on Islet Cell and Regenerative Biology, Joslin Diabetes Center, Boston, MA, USA; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA., Baek C; Department of Electrical Engineering and Computer Sciences, University of California, Berkeley, Berkeley, CA, USA., Mehlenbacher E; Boston, MA., Patel CJ; Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA. Electronic address: Chirag_Patel@hms.harvard.edu., Kostic AD; Section on Pathophysiology and Molecular Pharmacology, Joslin Diabetes Center, Boston, MA, USA; Section on Islet Cell and Regenerative Biology, Joslin Diabetes Center, Boston, MA, USA; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, USA. Electronic address: Aleksandar.Kostic@joslin.harvard.edu.
المصدر: Cell host & microbe [Cell Host Microbe] 2019 Aug 14; Vol. 26 (2), pp. 283-295.e8.
نوع المنشور: Journal Article; Meta-Analysis; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101302316 Publication Model: Print Cited Medium: Internet ISSN: 1934-6069 (Electronic) Linking ISSN: 19313128 NLM ISO Abbreviation: Cell Host Microbe Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, Mass. : Cell Press
مواضيع طبية MeSH: Metagenome/*genetics , Microbiota/*genetics , Microbiota/*physiology, Bacteria/classification ; Bacteria/genetics ; Biodiversity ; Cluster Analysis ; DNA Fingerprinting ; Databases, Factual ; Gastrointestinal Tract/microbiology ; Genetic Heterogeneity ; Host Microbial Interactions ; Humans ; Metagenomics ; Mouth/microbiology ; Multigene Family ; Phenotype
مستخلص: Despite substantial interest in the species diversity of the human microbiome and its role in disease, the scale of its genetic diversity, which is fundamental to deciphering human-microbe interactions, has not been quantified. Here, we conducted a cross-study meta-analysis of metagenomes from two human body niches, the mouth and gut, covering 3,655 samples from 13 studies. We found staggering genetic heterogeneity in the dataset, identifying a total of 45,666,334 non-redundant genes (23,961,508 oral and 22,254,436 gut) at the 95% identity level. Fifty percent of all genes were "singletons," or unique to a single metagenomic sample. Singletons were enriched for different functions (compared with non-singletons) and arose from sub-population-specific microbial strains. Overall, these results provide potential bases for the unexplained heterogeneity observed in microbiome-derived human phenotypes. One the basis of these data, we built a resource, which can be accessed at https://microbial-genes.bio.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: R00 ES023504 United States ES NIEHS NIH HHS; R01 AI127250 United States AI NIAID NIH HHS; T32 HG002295 United States HG NHGRI NIH HHS; T32 DK110919 United States DK NIDDK NIH HHS; K99 ES023504 United States ES NIEHS NIH HHS; R21 ES025052 United States ES NIEHS NIH HHS; P30 DK036836 United States DK NIDDK NIH HHS
فهرسة مساهمة: Keywords: de novo assembly; gene catalog; gene diversity; gut microbiome; metagenomics; microbial diversity; oral microbiome
تواريخ الأحداث: Date Created: 20190816 Date Completed: 20191219 Latest Revision: 20240719
رمز التحديث: 20240719
مُعرف محوري في PubMed: PMC6716383
DOI: 10.1016/j.chom.2019.07.008
PMID: 31415755
قاعدة البيانات: MEDLINE
الوصف
تدمد:1934-6069
DOI:10.1016/j.chom.2019.07.008