دورية أكاديمية
Feasibility of using RFLP of PCR-amplified 16S rRNA gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey.
| العنوان: | Feasibility of using RFLP of PCR-amplified 16S rRNA gene(s) for rapid differentiation of isolates of aerobic spore-forming bacteria from honey. |
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| المؤلفون: | López AC; Unidad de Bacteriología, Centro de Investigaciones de Fitopatología, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, cc 31, calle 60 y 119, 1900 La Plata, Argentina., Alippi AM; Unidad de Bacteriología, Centro de Investigaciones de Fitopatología, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, cc 31, calle 60 y 119, 1900 La Plata, Argentina. Electronic address: alippi@biol.unlp.edu.ar. |
| المصدر: | Journal of microbiological methods [J Microbiol Methods] 2019 Oct; Vol. 165, pp. 105690. Date of Electronic Publication: 2019 Aug 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8359 (Electronic) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, The Netherlands : Elsevier Biomedical, c1983- |
| مواضيع طبية MeSH: | Polymorphism, Restriction Fragment Length*, Bacterial Typing Techniques/*methods , DNA, Bacterial/*isolation & purification , Endospore-Forming Bacteria/*isolation & purification , Honey/*microbiology , Polymerase Chain Reaction/*methods, DNA, Bacterial/genetics ; Endospore-Forming Bacteria/classification ; Endospore-Forming Bacteria/genetics ; Feasibility Studies ; Phylogeny ; RNA, Ribosomal, 16S/genetics |
| مستخلص: | This study aimed to assess the feasibility of using RFLP of PCR-amplified 16S rRNA gene (s) by using universal primers 27f/1492r and a combination of three restriction enzymes, AluI, CfoI, and TaqI, for a low-cost, rapid screen for a primarily differentiation of isolates of the complex of aerobic spore-forming bacteria commonly found in honey samples. The described method produced unique and distinguishable patterns to differentiate among 80 isolates belonging to 26 different species of Bacillus, Brevibacillus, Lysinibacillus, Rummeliibacillus, and Paenibacillus reported in honey and other apiarian sources. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bacillus; Brevibacillus; Lysinibacillus; PCR-RFLP; Paenibacillus |
| المشرفين على المادة: | 0 (DNA, Bacterial) 0 (RNA, Ribosomal, 16S) |
| تواريخ الأحداث: | Date Created: 20190820 Date Completed: 20200722 Latest Revision: 20200722 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.mimet.2019.105690 |
| PMID: | 31425714 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1872-8359 |
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| DOI: | 10.1016/j.mimet.2019.105690 |