دورية أكاديمية
أعرض في EDS

Occurrence and genetic diversity of hemoplasmas in beef cattle from the Brazilian Pantanal, an endemic area for bovine trypanosomiasis in South America.

التفاصيل البيبلوغرافية
العنوان: Occurrence and genetic diversity of hemoplasmas in beef cattle from the Brazilian Pantanal, an endemic area for bovine trypanosomiasis in South America.
المؤلفون: de Mello VVC; Graduate Program in Agricultural Microbiology, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil; Laboratory of Immunoparasitology, Department of Veterinary Pathology, Faculdade de Ciências Agrárias e Veterinárias/ Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil., de Souza Ramos IA; Laboratory of Immunoparasitology, Department of Veterinary Pathology, Faculdade de Ciências Agrárias e Veterinárias/ Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil., Herrera HM; Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil., Mendes NS; Graduate Program in Agricultural Microbiology, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil; Laboratory of Immunoparasitology, Department of Veterinary Pathology, Faculdade de Ciências Agrárias e Veterinárias/ Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil., Calchi AC; Laboratory of Immunoparasitology, Department of Veterinary Pathology, Faculdade de Ciências Agrárias e Veterinárias/ Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil., Campos JBV; Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil., Macedo GC; Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil., Alves JVA; Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil., Machado RZ; Laboratory of Immunoparasitology, Department of Veterinary Pathology, Faculdade de Ciências Agrárias e Veterinárias/ Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil., André MR; Laboratory of Immunoparasitology, Department of Veterinary Pathology, Faculdade de Ciências Agrárias e Veterinárias/ Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil. Electronic address: mr.andre@unesp.br.
المصدر: Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2019 Oct; Vol. 66, pp. 101337. Date of Electronic Publication: 2019 Jul 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 7808924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1667 (Electronic) Linking ISSN: 01479571 NLM ISO Abbreviation: Comp Immunol Microbiol Infect Dis Subsets: MEDLINE
أسماء مطبوعة: Publication: Exeter : Elsevier Science Ltd
Original Publication: Oxford, Elmsford, N. Y., Pergamon Press.
مواضيع طبية MeSH: Genetic Variation*, Mycoplasma/*genetics , Mycoplasma Infections/*veterinary, Animals ; Antibodies, Protozoan/blood ; Brazil/epidemiology ; Cattle ; Cattle Diseases/microbiology ; Cattle Diseases/parasitology ; DNA, Bacterial/genetics ; Endemic Diseases/veterinary ; Female ; Genotype ; Immunoglobulin G/blood ; Male ; Mycoplasma/isolation & purification ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Trypanosoma vivax ; Trypanosomiasis, Bovine/epidemiology
مستخلص: Hemotropic mycoplasmas (hemoplasmas) are Gram-negative bacteria that parasitize the erythrocyte surface of a wide variety of mammals. The present study aimed at investigating the occurrence of hemoplasmas in beef cattle in the Brazilian Pantanal, an area endemic for bovine trypanosomiasis in South America. Additionally, the objective of this study was to characterize molecularly the genotypes of the found hemoplasmas. For this purpose, blood and serum samples of 400 beef cattle were collected from five properties in Corumbá, Nhecolândia sub-region, Mato Grosso do Sul, in Midwest Brazil. Blood samples underwent DNA extraction and standard 16S rRNA gene-based PCR assays for hemoplasmas. The sequences obtained were submitted to phylogenetic inferences, distance analysis, and genotype diversity. The Indirect Enzyme-Linked Immunoabsorbent Assay (iELISA) indicated the presence of anti-Trypanosoma vivax IgG antibodies in 89.75% of the animals sampled, confirming the endemicity of said agent in the studied region. Among the 400 bovine blood samples tested, 2.25% (9/400) were positive for hemoplasmas in cPCR. The phylogenetic analysis of the obtained sequences confirmed the presence of 'Candidatus Mycoplasma haemobos' and Mycoplasma wenyonii DNA in 0.5% (2/400) and 1.75% (7/400) animals, respectively. Five genotypes of M. wenyonii and one of 'Candidatus M. haemobos' were detected among the sequenced amplicons. The present study showed low molecular occurrence of haemoplasmas in beef cattle sampled in the Brazilian Pantanal, an area endemic for bovine trypanosomiasis. Despite of the conservation of the 16S rRNA gene, there was considerable diversity of hemoplasma genotypes infecting the sampled beef cattle.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: 16S rRNA; Beef cattle; Genotypic diversity; Hemotropic mycoplasmas; Pantanal’; Trypanosoma vivax
المشرفين على المادة: 0 (Antibodies, Protozoan)
0 (DNA, Bacterial)
0 (Immunoglobulin G)
0 (RNA, Ribosomal, 16S)
تواريخ الأحداث: Date Created: 20190823 Date Completed: 20200204 Latest Revision: 20200204
رمز التحديث: 20221213
DOI: 10.1016/j.cimid.2019.101337
PMID: 31437678
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-1667
DOI:10.1016/j.cimid.2019.101337