دورية أكاديمية
Polymorphism eNOS Glu298Asp modulates the inflammatory response of human peripheral blood mononuclear cells.
| العنوان: | Polymorphism eNOS Glu298Asp modulates the inflammatory response of human peripheral blood mononuclear cells. |
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| المؤلفون: | Maurer P; Postgraduate Program of Biochemistry, Federal University of Pampa, Uruguaiana, RS, Brazil., Barbisan F; Laboratory of Biogenomics, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil., Azzolin VF; Laboratory of Biogenomics, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil., Berro LF; Postgraduate Program of Biochemistry, Federal University of Pampa, Uruguaiana, RS, Brazil., Montagner R; Postgraduate Program of Pharmaceutical Sciences, Federal University of Pampa, Uruguaiana, RS, Brazil., Duarte MMMF; Lutheran University of Brazil, Santa Maria, RS, Brazil., da Cruz IBM; Laboratory of Biogenomics, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil., Manfredini V; Postgraduate Program of Biochemistry, Federal University of Pampa, Uruguaiana, RS, Brazil., Piccoli JDCE; Postgraduate Program of Biochemistry, Federal University of Pampa, Uruguaiana, RS, Brazil. Electronic address: jacquelinepiccoli@unipampa.edu.br. |
| المصدر: | Cytokine [Cytokine] 2020 Jan; Vol. 125, pp. 154812. Date of Electronic Publication: 2019 Aug 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 9005353 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0023 (Electronic) Linking ISSN: 10434666 NLM ISO Abbreviation: Cytokine Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2001- > : Oxford : Elsevier Science Ltd. Original Publication: [Philadelphia, PA] : Saunders Scientific Publications, W.B. Saunders, [c1989- |
| مواضيع طبية MeSH: | Cytokines/*metabolism , Leukocytes, Mononuclear/*metabolism , Nitric Oxide Synthase Type III/*genetics, Alleles ; Black People/genetics ; Genetic Association Studies ; Genotype ; Humans ; Inflammation/genetics ; Inflammation/metabolism ; Interleukin-1/metabolism ; Interleukin-6/metabolism ; Leukocytes, Mononuclear/drug effects ; Leukocytes, Mononuclear/immunology ; Mitogens/pharmacology ; Nitric Oxide/metabolism ; Phenotype ; Phytohemagglutinins/pharmacology ; Polymorphism, Single Nucleotide ; Tumor Necrosis Factor-alpha/metabolism |
| مستخلص: | Introduction: Nitric oxide is a gaseous radical produced by the nitric oxide endothelial synthase (eNOS) whose most studied physiological action is the vasodilation. However, it also acts in the defense of the organism through the formation of cytotoxic radicals, which can potentiate the inflammatory lesion of the cells. The Glu298Asp is a single nucleotide polymorphism (SNP) in the eNOS gene related to the risk of cardiovascular disease. Blacks present a higher prevalence of hypertension and cardiovascular mortality. Then, we aimed to evaluate the influence of Glu298Asp polymorphism on inflammatory response in vitro and gene expression in blacks. Material and Methods: Peripheral blood mononuclear cells (PBMC) from blacks with different Glu298Asp genotypes were treated with phytohemagglutinin (PHA), a mitogen and activator of T cells. Oxidative, inflammatory markers, and expression of inflammation genes were evaluated. Results: The genotype frequencies were TT 6.7%; TG 29.3% and GG 64.0%. Activation of PBMCs with 125 μg of PHA modulated the expression of inflammatory genes and increased levels of inflammatory cytokines. The T allele showed increased susceptibility to inflammation (higher levels of interleukin 1, interleukin 6 and tumor necrosis factor alpha; p < 0.001). The G allele exhibited protection through higher levels of nitric oxide (p < 0.001) and fewer inflammatory cytokines. Conclusion: Despite methodological limitations related to in vitro assays, the whole of results suggested that Glu298Asp modulates inflammatory genes, the T allele is more susceptible to inflammation and the G allele is protective. (Copyright © 2019 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Afrodescendant; Inflammation; Negroid race; eNOS |
| المشرفين على المادة: | 0 (Cytokines) 0 (IL6 protein, human) 0 (Interleukin-1) 0 (Interleukin-6) 0 (Mitogens) 0 (Phytohemagglutinins) 0 (Tumor Necrosis Factor-alpha) 31C4KY9ESH (Nitric Oxide) EC 1.14.13.39 (NOS3 protein, human) EC 1.14.13.39 (Nitric Oxide Synthase Type III) |
| تواريخ الأحداث: | Date Created: 20190824 Date Completed: 20210512 Latest Revision: 20221207 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.cyto.2019.154812 |
| PMID: | 31442681 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0023 |
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| DOI: | 10.1016/j.cyto.2019.154812 |