دورية أكاديمية

Nutrient enrichment induces a shift in dissolved organic carbon (DOC) metabolism in oligotrophic freshwater sediments.

التفاصيل البيبلوغرافية
العنوان: Nutrient enrichment induces a shift in dissolved organic carbon (DOC) metabolism in oligotrophic freshwater sediments.
المؤلفون: Brailsford FL; Environment Centre Wales, Bangor University, Bangor, Gwynedd LL57 2UW, UK; Centre for Environmental Biotechnology, Bangor University, Bangor, Gwynedd LL57 2UW, UK. Electronic address: f.brailsford@bangor.ac.uk., Glanville HC; Environment Centre Wales, Bangor University, Bangor, Gwynedd LL57 2UW, UK; School of Geography, Geology and the Environment, Keele University, Staffordshire ST5 5BG, UK., Golyshin PN; Environment Centre Wales, Bangor University, Bangor, Gwynedd LL57 2UW, UK; Centre for Environmental Biotechnology, Bangor University, Bangor, Gwynedd LL57 2UW, UK., Marshall MR; Environment Centre Wales, Bangor University, Bangor, Gwynedd LL57 2UW, UK., Lloyd CE; School of Chemistry, University of Bristol, University Road, Bristol BS8 1TS, UK., Johnes PJ; School of Geographical Sciences, University of Bristol, University Road, Bristol BS8 1SS, UK., Jones DL; Environment Centre Wales, Bangor University, Bangor, Gwynedd LL57 2UW, UK; UWA School of Agriculture and Environment, The University of Western Australia, Crawley, WA 6009, Australia.
المصدر: The Science of the total environment [Sci Total Environ] 2019 Nov 10; Vol. 690, pp. 1131-1139. Date of Electronic Publication: 2019 Jul 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0330500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1026 (Electronic) Linking ISSN: 00489697 NLM ISO Abbreviation: Sci Total Environ Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier.
مواضيع طبية MeSH: Environmental Monitoring*, Geologic Sediments/*chemistry , Water Pollutants/*analysis, Carbon/analysis ; Fresh Water/chemistry ; Nitrogen/analysis ; Phosphorus/analysis
مستخلص: Dissolved organic carbon (DOC) turnover in aquatic environments is modulated by the presence of other key macronutrients, including nitrogen (N) and phosphorus (P). The ratio of these nutrients directly affects the rates of microbial growth and nutrient processing in the natural environment. The aim of this study was to investigate how labile DOC metabolism responds to changes in nutrient stoichiometry using 14 C tracers in conjunction with untargeted analysis of the primary metabolome in upland peat river sediments. N addition led to an increase in 14 C-glucose uptake, indicating that the sediments were likely to be primarily N limited. The mineralisation of glucose to 14 CO 2 reduced following N addition, indicating that nutrient addition induced shifts in internal carbon (C) partitioning and microbial C use efficiency (CUE). This is directly supported by the metabolomic profile data which identified significant differences in 22 known metabolites (34% of the total) and 30 unknown metabolites (16% of the total) upon the addition of either N or P. 14 C-glucose addition increased the production of organic acids known to be involved in mineral P dissolution (e.g. gluconic acid, malic acid). Conversely, when N was not added, the addition of glucose led to the production of the sugar alcohols, mannitol and sorbitol, which are well known microbial C storage compounds. P addition resulted in increased levels of several amino acids (e.g. alanine, glycine) which may reflect greater rates of microbial growth or the P requirement for coenzymes required for amino acid synthesis. We conclude that inorganic nutrient enrichment in addition to labile C inputs has the potential to substantially alter in-stream biogeochemical cycling in oligotrophic freshwaters.
(Copyright © 2019. Published by Elsevier B.V.)
فهرسة مساهمة: Keywords: DOM processing; Dissolved organic matter; Metabolic profiling; Nutrient availability; Stoichiometry
المشرفين على المادة: 0 (Water Pollutants)
27YLU75U4W (Phosphorus)
7440-44-0 (Carbon)
N762921K75 (Nitrogen)
تواريخ الأحداث: Date Created: 20190901 Date Completed: 20191023 Latest Revision: 20191023
رمز التحديث: 20240628
DOI: 10.1016/j.scitotenv.2019.07.054
PMID: 31470476
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-1026
DOI:10.1016/j.scitotenv.2019.07.054