دورية أكاديمية
أعرض في EDS

MaPacC, a pH-responsive transcription factor, negatively regulates thermotolerance and contributes to conidiation and virulence in Metarhizium acridum.

التفاصيل البيبلوغرافية
العنوان: MaPacC, a pH-responsive transcription factor, negatively regulates thermotolerance and contributes to conidiation and virulence in Metarhizium acridum.
المؤلفون: Zhang M; Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing, 401331, People's Republic of China.; Chongqing Engineering Research Center for Fungal Insecticide, Chongqing, 401331, People's Republic of China.; Key Laboratory of Gene Function and Regulation Technologies Under Chongqing Municipal Education Commission, Chongqing, 401331, People's Republic of China., Wei Q; Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing, 401331, People's Republic of China.; Chongqing Engineering Research Center for Fungal Insecticide, Chongqing, 401331, People's Republic of China.; Key Laboratory of Gene Function and Regulation Technologies Under Chongqing Municipal Education Commission, Chongqing, 401331, People's Republic of China., Xia Y; Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing, 401331, People's Republic of China. yuxianxia@cqu.edu.cn.; Chongqing Engineering Research Center for Fungal Insecticide, Chongqing, 401331, People's Republic of China. yuxianxia@cqu.edu.cn.; Key Laboratory of Gene Function and Regulation Technologies Under Chongqing Municipal Education Commission, Chongqing, 401331, People's Republic of China. yuxianxia@cqu.edu.cn., Jin K; Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing, 401331, People's Republic of China. jinkai@cqu.edu.cn.; Chongqing Engineering Research Center for Fungal Insecticide, Chongqing, 401331, People's Republic of China. jinkai@cqu.edu.cn.; Key Laboratory of Gene Function and Regulation Technologies Under Chongqing Municipal Education Commission, Chongqing, 401331, People's Republic of China. jinkai@cqu.edu.cn.
المصدر: Current genetics [Curr Genet] 2020 Apr; Vol. 66 (2), pp. 397-408. Date of Electronic Publication: 2019 Aug 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer International Country of Publication: United States NLM ID: 8004904 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0983 (Electronic) Linking ISSN: 01728083 NLM ISO Abbreviation: Curr Genet Subsets: MEDLINE
أسماء مطبوعة: Publication: New York Ny : Springer International
Original Publication: [New York] Springer International.
مواضيع طبية MeSH: Thermotolerance*, Grasshoppers/*microbiology , Metarhizium/*metabolism , Transcription Factors/*metabolism, Animals ; Fungal Proteins/metabolism ; Gene Expression Regulation, Fungal ; Grasshoppers/immunology ; Hemolymph ; Metarhizium/genetics ; Metarhizium/pathogenicity ; Metarhizium/physiology ; Mycoses/immunology ; Virulence/genetics
مستخلص: PacC is a pH-responsive transcription factor gene highly expressed at alkaline pH and plays distinct roles in environmental fitness, conidiation and virulence of different fungi. Here, we show biological functions of orthologous MaPacC in the locust-specific fungal pathogen Metarhizium acridum. Disruption of MapacC slowed down the fungal growth only under alkaline conditions. Intriguingly, the fungal thermotolerance was enhanced by the MapacC deletion, accompanied by transcriptional upregulation of some heat shock-responsive genes. The disruptant suffered a reduction in conidial yield and a change in conidial surface structure, but showed little change in cell wall integrity. The virulence of the disruptant against a locust species was markedly attenuated due to delayed appressorium formation, repressed expression of some insect cuticle hydrolases and slowed growth in locust hemolymph. The phenoloxidase activity and nodules of the locusts infected by the disruptant were also boosted. All of these phenotypic changes were restored by targeted gene complementation. Our results indicate that MaPacC acts a negative regulator of thermotolerance and contributes to the virulence of M. acridum by an involvement in hyphal penetration through insect cuticle and evasion from insect immunity.
References: Immunol Rev. 2004 Apr;198:116-26. (PMID: 15199959)
Microbiology. 2012 Dec;158(Pt 12):2987-2996. (PMID: 23038805)
J Invertebr Pathol. 2005 May;89(1):19-29. (PMID: 16039302)
PLoS One. 2013 Jul 16;8(7):e69236. (PMID: 23874922)
Pest Manag Sci. 2018 Apr;74(4):781-789. (PMID: 28905488)
J Mol Biol. 2003 Dec 5;334(4):667-84. (PMID: 14636595)
Appl Microbiol Biotechnol. 2018 Jun;102(12):4995-5004. (PMID: 29704043)
Environ Microbiol. 2010 Jul;12(7):1868-77. (PMID: 20236165)
J Biol Chem. 2007 Nov 30;282(48):34735-47. (PMID: 17911112)
Microbiology. 1999 Nov;145 ( Pt 11):3229-3234. (PMID: 10589732)
EMBO J. 1995 Feb 15;14(4):779-90. (PMID: 7882981)
Insect Sci. 2013 Apr;20(2):254-60. (PMID: 23955865)
J Invertebr Pathol. 2005 Sep;90(1):55-8. (PMID: 16005467)
Can J Microbiol. 2006 Jul;52(7):623-6. (PMID: 16917517)
FEMS Microbiol Lett. 2009 Feb;291(1):127-35. (PMID: 19076228)
Curr Genet. 2018 Jun;64(3):529-534. (PMID: 29119270)
Appl Microbiol Biotechnol. 2016 May;100(10):4423-33. (PMID: 26754817)
Mol Microbiol. 2005 Feb;55(4):1072-84. (PMID: 15686555)
Curr Opin Microbiol. 2014 Dec;22:49-59. (PMID: 25460796)
Eukaryot Cell. 2005 Jun;4(6):999-1008. (PMID: 15947192)
Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6647-52. (PMID: 16614065)
Infect Immun. 2000 Oct;68(10):5953-9. (PMID: 10992507)
J Biol Chem. 2007 Jul 20;282(29):21110-5. (PMID: 17526497)
J Insect Physiol. 2003 Aug;49(8):795-803. (PMID: 12880660)
Appl Microbiol Biotechnol. 2014 Jun;98(12):5589-97. (PMID: 24769907)
Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19267-72. (PMID: 18032603)
Microbiology. 2007 Oct;153(Pt 10):3448-3457. (PMID: 17906143)
Curr Genet. 2015 Aug;61(3):427-40. (PMID: 25986971)
Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6349-54. (PMID: 8692818)
J Biol Chem. 2000 Feb 18;275(7):4995-5002. (PMID: 10671539)
Appl Microbiol Biotechnol. 2018 Jul;102(13):5611-5623. (PMID: 29713793)
Curr Genet. 2015 Aug;61(3):383-404. (PMID: 25791499)
Microbiol Mol Biol Rev. 2002 Sep;66(3):426-46, table of contents. (PMID: 12208998)
Fungal Genet Biol. 2011 Mar;48(3):275-84. (PMID: 21126599)
Trends Genet. 2003 Apr;19(4):224-31. (PMID: 12683976)
Trends Microbiol. 1996 May;4(5):197-203. (PMID: 8727600)
Curr Genet. 1998 Dec;34(5):404-9. (PMID: 9871124)
Curr Genet. 2015 Aug;61(3):405-25. (PMID: 25824285)
Microbiology. 2009 Sep;155(Pt 9):3121-3133. (PMID: 19608611)
Appl Environ Microbiol. 2005 Jan;71(1):363-70. (PMID: 15640210)
Mol Plant Microbe Interact. 2003 Sep;16(9):785-95. (PMID: 12971602)
Exp Parasitol. 2012 Mar;130(3):300-5. (PMID: 22143088)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
J Mol Biol. 1997 Dec 12;274(4):466-80. (PMID: 9417928)
Eukaryot Cell. 2014 Mar;13(3):342-52. (PMID: 24442891)
Trends Microbiol. 2008 Jun;16(6):291-300. (PMID: 18457952)
PLoS Genet. 2011 Jan 06;7(1):e1001264. (PMID: 21253567)
Nat Rev Microbiol. 2011 Dec 12;10(2):112-22. (PMID: 22158429)
Mol Plant Microbe Interact. 2007 Sep;20(9):1149-60. (PMID: 17849717)
Mol Microbiol. 2003 May;48(3):765-79. (PMID: 12694620)
Environ Microbiol. 2015 Apr;17(4):994-1008. (PMID: 24612440)
Appl Microbiol Biotechnol. 2017 Dec;101(23-24):8571-8584. (PMID: 29079863)
Environ Microbiol. 2017 Feb;19(2):788-802. (PMID: 28083986)
Annu Rev Genet. 1993;27:437-96. (PMID: 8122909)
معلومات مُعتمدة: 31471820 the Natural Science Foundation of China; cstc2015jcyja80037 Natural Science Foundation of Chongqing; cstc2018jcyjAX0554 Natural Science Foundation of Chongqing
فهرسة مساهمة: Keywords: Conidial surface structures; Insect immune evasion; Metarhizium acridum; PacC; Thermotolerance; Virulence
المشرفين على المادة: 0 (Fungal Proteins)
0 (Transcription Factors)
SCR Organism: Metarhizium acridum
تواريخ الأحداث: Date Created: 20190901 Date Completed: 20210101 Latest Revision: 20210101
رمز التحديث: 20231215
DOI: 10.1007/s00294-019-01032-3
PMID: 31471639
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-0983
DOI:10.1007/s00294-019-01032-3