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Molecular epidemiology of Leptospira noguchii reveals important insights into a One Health context.

التفاصيل البيبلوغرافية
العنوان: Molecular epidemiology of Leptospira noguchii reveals important insights into a One Health context.
المؤلفون: Loureiro AP; Laboratório de Bacteriologia Veterinária, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, Brazil., Jaeger LH; Laboratório de Bacteriologia Veterinária, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, Brazil.; Laboratório de Epidemiologia e Sistemática Molecular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., Di Azevedo MIN; Laboratório de Bacteriologia Veterinária, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, Brazil., Miraglia F; Laboratório de Bacteriologia Veterinária, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, Brazil.; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Moreno LZ; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Moreno AM; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil., Pestana CP; Laboratório de Tecnologia Recombinante, Bio-Manguinhos, FIOCRUZ, Rio de Janeiro, Brazil., Carvalho-Costa FA; Laboratório de Epidemiologia e Sistemática Molecular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., Medeiros MA; Laboratório de Tecnologia Recombinante, Bio-Manguinhos, FIOCRUZ, Rio de Janeiro, Brazil., Lilenbaum W; Laboratório de Bacteriologia Veterinária, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense, Niterói, Brazil.
المصدر: Transboundary and emerging diseases [Transbound Emerg Dis] 2020 Jan; Vol. 67 (1), pp. 276-283. Date of Electronic Publication: 2019 Sep 17.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Blackwell Verlag Country of Publication: Germany NLM ID: 101319538 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1865-1682 (Electronic) Linking ISSN: 18651674 NLM ISO Abbreviation: Transbound Emerg Dis Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin : Blackwell Verlag
مواضيع طبية MeSH: One Health*, Cattle Diseases/*epidemiology , Leptospira/*genetics , Leptospirosis/*veterinary, Animals ; Bacterial Typing Techniques/veterinary ; Cattle ; Cattle Diseases/microbiology ; Electrophoresis, Gel, Pulsed-Field/veterinary ; Female ; Genotype ; Humans ; Leptospira/classification ; Leptospira/immunology ; Leptospira/pathogenicity ; Leptospirosis/epidemiology ; Leptospirosis/microbiology ; Molecular Epidemiology ; Multilocus Sequence Typing/veterinary ; Panama/epidemiology ; Phylogeny ; Serogroup ; Virulence ; Zoonoses
مستخلص: Leptospirosis presents a complex and dynamic epidemiology. Bovine leptospirosis has been described as a major infectious disease impairing reproductive efficiency. Although infections by Leptospira interrogans, L. santarosai and L. borgpetersenii are frequently reported in cattle, the presence of L. noguchii in these animals should not be neglected. In this study, we describe serological (MAT) and molecular characterization (rrs and secY gene sequencing, multilocus sequence typing [MLST] and pulsed-field gel electrophoresis [PFGE]) of eight L. noguchii strains obtained from slaughtered cows. Intraspecific genetic diversity was evaluated, and haplotype networks were constructed based on hosts and geographical localizations. Strains were characterized as belonging to serogroups Australis, Autumnalis and Panama, and molecular characterization showed a high heterogeneity of these strains. Ten different STs were found (including nine new STs and 39 novel alleles) as well as nine different pulsotypes. Two clonal complexes were found. Phylogenetic trees based on secY locus and concatenated MLST loci showed two main clusters, with sequences from the present study included in the first. In general, there was no relationship between the geographical origin and the secY phylogenetic clusters, as well as between secY phylogenetic clusters and serogroups. Molecular diversity indexes confirmed a high variability (H > 0.8). This high intraspecific variation observed may be related to differences in virulence, pathogenicity and antigenicity or even adaptability of the strains. In addition, haplotype networks clearly demonstrated the circulation of genotypes between humans and animals, confirming the zoonotic potential. The present study provides relevant data for the study of leptospirosis in the One Health context, where human, animal and environmental health is closely connected.
(© 2019 Blackwell Verlag GmbH.)
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معلومات مُعتمدة: 2011/18290-0 Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro; E-26/202.766/2016 Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro; E-26/202.370/2018 MINDA; 2013/17136-2 Fundação de Amparo à Pesquisa do Estado de São Paulo
فهرسة مساهمة: Keywords: Leptospira noguchii; MLST; PFGE; bovine leptospirosis; genotyping
سلسلة جزيئية: GENBANK KY113317; KY113324; MK579498; MK579566
تواريخ الأحداث: Date Created: 20190905 Date Completed: 20200526 Latest Revision: 20200526
رمز التحديث: 20221213
DOI: 10.1111/tbed.13349
PMID: 31484225
قاعدة البيانات: MEDLINE
الوصف
تدمد:1865-1682
DOI:10.1111/tbed.13349