دورية أكاديمية
Use of Liquid Patient Ascites Fluids as a Preclinical Model for Oncolytic Virus Activity.
| العنوان: | Use of Liquid Patient Ascites Fluids as a Preclinical Model for Oncolytic Virus Activity. |
|---|---|
| المؤلفون: | Scott EM; Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, UK., Frost S; Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, UK., Khalique H; Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, UK., Freedman JD; Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, UK., Seymour LW; Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, UK., Lei-Rossmann J; Department of Oncology, University of Oxford, Roosevelt Drive, Oxford, UK. janet.lei@oncology.ox.ac.uk. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2058, pp. 261-270. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Ascites* , Ascitic Fluid* , Genetic Vectors*/genetics , Liquid Biopsy*/methods , Oncolytic Virotherapy*/methods , Oncolytic Viruses*/genetics, Adenoviridae/genetics ; Biomarkers ; Epithelial Cell Adhesion Molecule/genetics ; Epithelial Cell Adhesion Molecule/metabolism ; Humans ; Immunophenotyping ; Neoplasms/diagnosis ; Neoplasms/therapy |
| مستخلص: | The translational success of oncolytic virotherapies would benefit from the widespread use of clinically relevant ex vivo models. Malignant ascites, an accumulation of fluid in the peritoneum due to disseminated cancer, recapitulates many features of the tumor microenvironment, making it a valuable model for studying oncolytic virus activity. Here, we describe a method for the separation and storage of cellular and acellular components of malignant ascites, followed by flow cytometric characterization of the cellular fraction. We then outline a simple experiment using whole ascites to assess the activity of a bispecific T cell engager (BiTE)-expressing oncolytic adenovirus. |
| معلومات مُعتمدة: | 29106 United Kingdom CRUK_ Cancer Research UK; C5255/A20936 United Kingdom CRUK_ Cancer Research UK; C552/A17720 United Kingdom CRUK_ Cancer Research UK; MR/N013468/1 United Kingdom MRC_ Medical Research Council |
| فهرسة مساهمة: | Keywords: Ascites; Ex vivo model; Flow cytometry; Liquid biopsies; Oncolytic virus |
| المشرفين على المادة: | 0 (Biomarkers) 0 (EPCAM protein, human) 0 (Epithelial Cell Adhesion Molecule) |
| تواريخ الأحداث: | Date Created: 20190906 Date Completed: 20201214 Latest Revision: 20240210 |
| رمز التحديث: | 20240210 |
| DOI: | 10.1007/978-1-4939-9794-7_17 |
| PMID: | 31486044 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
|---|---|
| DOI: | 10.1007/978-1-4939-9794-7_17 |