دورية أكاديمية
أعرض في EDS

Programmed Chromosome Deletion in the Ciliate Oxytricha trifallax .

التفاصيل البيبلوغرافية
العنوان: Programmed Chromosome Deletion in the Ciliate Oxytricha trifallax .
المؤلفون: Clay DM; Departments of Biochemistry and Molecular Biophysics and Biological Sciences, Columbia University, New York, NY and.; Department of Molecular Biology, Princeton University, Princeton, NJ., Yerlici VT; Departments of Biochemistry and Molecular Biophysics and Biological Sciences, Columbia University, New York, NY and.; Department of Molecular Biology, Princeton University, Princeton, NJ., Villano DJ; Departments of Biochemistry and Molecular Biophysics and Biological Sciences, Columbia University, New York, NY and., Landweber LF; Departments of Biochemistry and Molecular Biophysics and Biological Sciences, Columbia University, New York, NY and Laura.Landweber@columbia.edu.
المصدر: G3 (Bethesda, Md.) [G3 (Bethesda)] 2019 Oct 07; Vol. 9 (10), pp. 3105-3118. Date of Electronic Publication: 2019 Oct 07.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 101566598 Publication Model: Electronic Cited Medium: Internet ISSN: 2160-1836 (Electronic) Linking ISSN: 21601836 NLM ISO Abbreviation: G3 (Bethesda) Subsets: MEDLINE
أسماء مطبوعة: Publication: 2021- : [Oxford] : Oxford University Press
Original Publication: Bethesda, MD : Genetics Society of America, 2011-
مواضيع طبية MeSH: Chromosome Deletion*, Oxytricha/*genetics, DNA Fragmentation ; Epigenesis, Genetic ; Gene Rearrangement ; Genome, Protozoan ; Genomics/methods ; High-Throughput Nucleotide Sequencing
مستخلص: The ciliate Oxytricha trifallax contains two nuclei: a germline micronucleus and a somatic macronucleus. These two nuclei diverge significantly in genomic structure. The micronucleus contains approximately 100 chromosomes of megabase scale, while the macronucleus contains 16,000 gene-sized, high ploidy "nanochromosomes." During its sexual cycle, a copy of the zygotic germline micronucleus develops into a somatic macronucleus via DNA excision and rearrangement. The rearrangement process is guided by multiple RNA-based pathways that program the epigenetic inheritance of sequences in the parental macronucleus of the subsequent generation. Here, we show that the introduction of synthetic DNA molecules homologous to a complete native nanochromosome during the rearrangement process results in either loss or heavy copy number reduction of the targeted nanochromosome in the macronucleus of the subsequent generation. This phenomenon was tested on a variety of nanochromosomes with different micronuclear structures, with deletions resulting in all cases. Deletion of the targeted nanochromosome results in the loss of expression of the targeted genes, including gene knockout phenotypes that were phenocopied using alternative knockdown approaches. Further investigation of the chromosome deletion showed that, although the full length nanochromosome was lost, remnants of the targeted chromosome remain. We were also able to detect the presence of telomeres on these remnants. The chromosome deletions and remnants are epigenetically inherited when backcrossed to wild type strains, suggesting that an undiscovered mechanism programs DNA elimination and cytoplasmically transfers to both daughter cells during conjugation. Programmed deletion of targeted chromosomes provides a novel approach to investigate genome rearrangement and expands the available strategies for gene knockout in Oxytricha trifallax .
(Copyright © 2019 Clay et al.)
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معلومات مُعتمدة: R01 GM059708 United States GM NIGMS NIH HHS; R01 GM111933 United States GM NIGMS NIH HHS; R35 GM122555 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: Ciliates; Epigenetics; Genomic Rearrangement
تواريخ الأحداث: Date Created: 20190912 Date Completed: 20200312 Latest Revision: 20240216
رمز التحديث: 20240216
مُعرف محوري في PubMed: PMC6778801
DOI: 10.1534/g3.118.200930
PMID: 31506317
قاعدة البيانات: MEDLINE
الوصف
تدمد:2160-1836
DOI:10.1534/g3.118.200930