دورية أكاديمية
MbnH is a diheme MauG-like protein associated with microbial copper homeostasis.
| العنوان: | MbnH is a diheme MauG-like protein associated with microbial copper homeostasis. |
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| المؤلفون: | Kenney GE; Departments of Molecular Biosciences and Chemistry, Northwestern University, Evanston, Illinois 60208., Dassama LMK; Departments of Molecular Biosciences and Chemistry, Northwestern University, Evanston, Illinois 60208., Manesis AC; Departments of Molecular Biosciences and Chemistry, Northwestern University, Evanston, Illinois 60208., Ross MO; Departments of Molecular Biosciences and Chemistry, Northwestern University, Evanston, Illinois 60208., Chen S; Departments of Molecular Biosciences and Chemistry, Northwestern University, Evanston, Illinois 60208., Hoffman BM; Departments of Molecular Biosciences and Chemistry, Northwestern University, Evanston, Illinois 60208., Rosenzweig AC; Departments of Molecular Biosciences and Chemistry, Northwestern University, Evanston, Illinois 60208 amyr@northwestern.edu. |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2019 Nov 01; Vol. 294 (44), pp. 16141-16151. Date of Electronic Publication: 2019 Sep 11. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Copper/*metabolism , Imidazoles/*metabolism , Methylosinus trichosporium/*metabolism , Oligopeptides/*genetics , Oligopeptides/*metabolism, Amino Acid Sequence/genetics ; Bacteria/metabolism ; Bacterial Proteins/metabolism ; Computational Biology/methods ; Homeostasis ; Oligopeptides/biosynthesis ; Operon/genetics ; Protein Processing, Post-Translational |
| مستخلص: | Methanobactins (Mbns) are ribosomally-produced, post-translationally modified peptidic copper-binding natural products produced under conditions of copper limitation. Genes encoding Mbn biosynthetic and transport proteins have been identified in a wide variety of bacteria, indicating a broader role for Mbns in bacterial metal homeostasis. Many of the genes in the Mbn operons have been assigned functions, but two genes usually present, mbnP and mbnH , encode uncharacterized proteins predicted to reside in the periplasm. MbnH belongs to the bacterial diheme cytochrome c peroxidase (bCcP)/MauG protein family, and MbnP contains no domains of known function. Here, we performed a detailed bioinformatic analysis of both proteins and have biochemically characterized MbnH from Methylosinus ( Ms. ) trichosporium OB3b. We note that the mbnH and mbnP genes typically co-occur and are located proximal to genes associated with microbial copper homeostasis. Our bioinformatics analysis also revealed that the bCcP/MauG family is significantly more diverse than originally appreciated, and that MbnH is most closely related to the MauG subfamily. A 2.6 Å resolution structure of Ms. trichosporium OB3b MbnH combined with spectroscopic data and peroxidase activity assays provided evidence that MbnH indeed more closely resembles MauG than bCcPs, although its redox properties are significantly different from those of MauG. The overall similarity of MbnH to MauG suggests that MbnH could post-translationally modify a macromolecule, such as internalized CuMbn or its uncharacterized partner protein, MbnP. Our results indicate that MbnH is a MauG-like diheme protein that is likely involved in microbial copper homeostasis and represents a new family within the bCcP/MauG superfamily. (© 2019 Kenney et al.) |
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| معلومات مُعتمدة: | F32 GM110934 United States GM NIGMS NIH HHS; R01 GM111097 United States GM NIGMS NIH HHS; R35 GM118035 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: MauG; MbnH; bioinformatics; crystal structure; diheme cytochrome c peroxidase; heme; metal homeostasis; metalloprotein; methanobactin |
| سلسلة جزيئية: | PDB 3L4M |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Imidazoles) 0 (Oligopeptides) 0 (methanobactin) 789U1901C5 (Copper) |
| تواريخ الأحداث: | Date Created: 20190913 Date Completed: 20200720 Latest Revision: 20230107 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC6827288 |
| DOI: | 10.1074/jbc.RA119.010202 |
| PMID: | 31511324 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1083-351X |
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| DOI: | 10.1074/jbc.RA119.010202 |