دورية أكاديمية
أعرض في EDS

A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity.

التفاصيل البيبلوغرافية
العنوان: A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity.
المؤلفون: Lopez VA; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Park BC; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Nowak D; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland., Sreelatha A; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Zembek P; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland., Fernandez J; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Servage KA; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Howard Hughes Medical Institute, Dallas, TX 75390, USA., Gradowski M; Warsaw University of Life Sciences, Warsaw, Poland., Hennig J; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland., Tomchick DR; Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA., Pawłowski K; Warsaw University of Life Sciences, Warsaw, Poland; Lund University, Lund, Sweden., Krzymowska M; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland., Tagliabracci VS; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: vincent.tagliabracci@utsouthwestern.edu.
المصدر: Cell [Cell] 2019 Sep 19; Vol. 179 (1), pp. 205-218.e21. Date of Electronic Publication: 2019 Sep 12.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4172 (Electronic) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge, Ma : Cell Press
Original Publication: Cambridge, MIT Press.
مواضيع طبية MeSH: Arabidopsis Proteins/*metabolism , Bacterial Proteins/*metabolism , HSP90 Heat-Shock Proteins/*metabolism , Molecular Mimicry/*immunology , Plant Immunity/*physiology, Adenosine Triphosphatases/metabolism ; Arabidopsis/immunology ; Arabidopsis/metabolism ; Arabidopsis/microbiology ; Bacterial Proteins/chemistry ; HEK293 Cells ; HSP90 Heat-Shock Proteins/chemistry ; HeLa Cells ; Host Microbial Interactions/immunology ; Humans ; Phosphorylation ; Plasmids/genetics ; Protein Binding ; Protein Folding ; Protein Kinases/metabolism ; Pseudomonas syringae/metabolism ; Saccharomyces cerevisiae/metabolism
مستخلص: The molecular chaperone HSP90 facilitates the folding of several client proteins, including innate immune receptors and protein kinases. HSP90 is an essential component of plant and animal immunity, yet pathogenic strategies that directly target the chaperone have not been described. Here, we identify the HopBF1 family of bacterial effectors as eukaryotic-specific HSP90 protein kinases. HopBF1 adopts a minimal protein kinase fold that is recognized by HSP90 as a host client. As a result, HopBF1 phosphorylates HSP90 to completely inhibit the chaperone's ATPase activity. We demonstrate that phosphorylation of HSP90 prevents activation of immune receptors that trigger the hypersensitive response in plants. Consequently, HopBF1-dependent phosphorylation of HSP90 is sufficient to induce severe disease symptoms in plants infected with the bacterial pathogen, Pseudomonas syringae. Collectively, our results uncover a family of bacterial effector kinases with toxin-like properties and reveal a previously unrecognized betrayal mechanism by which bacterial pathogens modulate host immunity.
(Copyright © 2019 Elsevier Inc. All rights reserved.)
التعليقات: Comment in: Nat Plants. 2019 Nov;5(11):1110-1111. (PMID: 31685952)
References: Cell. 1999 Nov 12;99(4):355-66. (PMID: 10571178)
Electrophoresis. 1999 Dec;20(18):3551-67. (PMID: 10612281)
Nat Cell Biol. 2001 Jan;3(1):93-6. (PMID: 11146632)
EMBO J. 2001 Oct 15;20(20):5556-67. (PMID: 11597999)
EMBO Rep. 2001 Dec;2(12):1133-8. (PMID: 11743028)
Science. 2002 Mar 15;295(5562):2073-6. (PMID: 11847307)
Science. 2002 Mar 15;295(5562):2077-80. (PMID: 11847308)
Trends Mol Med. 2002;8(4 Suppl):S55-61. (PMID: 11927289)
Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 2):1772-9. (PMID: 12351820)
Science. 2002 Dec 6;298(5600):1912-34. (PMID: 12471243)
Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11777-82. (PMID: 14504384)
Nature. 2003 Oct 16;425(6959):737-41. (PMID: 14562106)
J Biol Chem. 2004 Jan 16;279(3):2101-8. (PMID: 14583611)
EMBO J. 2003 Nov 3;22(21):5679-89. (PMID: 14592967)
Curr Biol. 2004 Jan 6;14(1):R22-4. (PMID: 14711430)
Anal Biochem. 2004 Apr 15;327(2):176-83. (PMID: 15051534)
Genome Res. 2004 Jun;14(6):1188-90. (PMID: 15173120)
Structure. 2004 Jul;12(7):1303-11. (PMID: 15242606)
Plant Cell. 2004 Dec;16(12):3480-95. (PMID: 15548741)
Nat Struct Mol Biol. 2005 Feb;12(2):120-6. (PMID: 15643424)
Curr Opin Genet Dev. 2005 Feb;15(1):55-61. (PMID: 15661534)
Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14046-51. (PMID: 16162672)
J Biol Chem. 2006 May 19;281(20):14361-9. (PMID: 16551624)
Nature. 2006 Apr 20;440(7087):1013-7. (PMID: 16625188)
Proc Natl Acad Sci U S A. 2006 Jun 6;103(23):8575-6. (PMID: 16735473)
Acta Crystallogr D Biol Crystallogr. 2006 Aug;62(Pt 8):859-66. (PMID: 16855301)
Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1002-11. (PMID: 16929101)
Mol Microbiol. 2006 Oct;62(1):26-44. (PMID: 16942603)
Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6764-9. (PMID: 17420470)
Nat Immunol. 2007 May;8(5):497-503. (PMID: 17435760)
Sci STKE. 2007 May 08;2007(385):pe22. (PMID: 17488976)
Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. (PMID: 17690442)
Trends Cell Biol. 1997 Apr;7(4):157-61. (PMID: 17708934)
Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19262-6. (PMID: 18040046)
Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W465-9. (PMID: 18424797)
EMBO J. 2008 Oct 22;27(20):2789-98. (PMID: 18818696)
Annu Rev Plant Biol. 2009;60:139-64. (PMID: 19014346)
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674. (PMID: 19461840)
Proc Natl Acad Sci U S A. 2009 Jun 16;106(24):9556-63. (PMID: 19487680)
Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20330-5. (PMID: 19933325)
PLoS Pathog. 2009 Dec;5(12):e1000708. (PMID: 20041225)
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21. (PMID: 20057044)
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21. (PMID: 20124702)
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):470-8. (PMID: 20383000)
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501. (PMID: 20383002)
Nucleic Acids Res. 2010 Jul;38(Web Server issue):W545-9. (PMID: 20457744)
Nat Rev Mol Cell Biol. 2010 Jul;11(7):515-28. (PMID: 20531426)
Trends Biochem Sci. 2011 Feb;36(2):65-77. (PMID: 20971646)
Acta Crystallogr D Biol Crystallogr. 2010 Nov;66(Pt 11):1153-63. (PMID: 21041930)
Science. 2010 Nov 19;330(6007):1061-4. (PMID: 21097929)
Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):2975-80. (PMID: 21282655)
Nat Immunol. 2011 Apr;12(4):335-43. (PMID: 21399639)
Proc Natl Acad Sci U S A. 2011 May 3;108(18):7619-24. (PMID: 21490299)
PLoS One. 2011 Apr 27;6(4):e18910. (PMID: 21556138)
PLoS Pathog. 2011 Jul;7(7):e1002132. (PMID: 21799664)
Biochim Biophys Acta. 2012 Mar;1823(3):683-8. (PMID: 21871502)
Biochim Biophys Acta. 2012 Mar;1823(3):689-97. (PMID: 22001401)
J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712)
Annu Rev Biochem. 2012;81:587-613. (PMID: 22482904)
Science. 2012 Jun 1;336(6085):1150-3. (PMID: 22582013)
Biol Direct. 2012 Jun 25;7:18. (PMID: 22731697)
Case Rep Infect Dis. 2012;2012:730720. (PMID: 22762003)
BMC Microbiol. 2012 Jul 16;12:141. (PMID: 22800299)
Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834)
Cell. 2012 Aug 31;150(5):987-1001. (PMID: 22939624)
Cold Spring Harb Perspect Biol. 2012 Sep 01;4(9):a006114. (PMID: 22952390)
Mol Biol Evol. 2013 Apr;30(4):772-80. (PMID: 23329690)
Plant Physiol. 2013 Apr;161(4):2049-61. (PMID: 23396834)
PLoS One. 2013;8(2):e57558. (PMID: 23469023)
Nucleic Acids Res. 2013 Jul;41(12):6232-49. (PMID: 23620288)
Annu Rev Phytopathol. 2013;51:473-98. (PMID: 23725467)
Science. 2013 Aug 16;341(6147):746-51. (PMID: 23950531)
Bioinformatics. 2014 Mar 1;30(5):660-7. (PMID: 24130308)
Nucleic Acids Res. 2014 Jan;42(Database issue):D297-303. (PMID: 24319143)
Structure. 2014 Feb 4;22(2):250-9. (PMID: 24373767)
EMBO J. 2014 Mar 3;33(5):437-49. (PMID: 24446487)
Cell Host Microbe. 2014 Sep 10;16(3):364-75. (PMID: 25211078)
Annu Rev Plant Biol. 2015;66:487-511. (PMID: 25494461)
Nucleic Acids Res. 2015 Jul 1;43(W1):W30-8. (PMID: 25943547)
Nat Protoc. 2015 Jun;10(6):845-58. (PMID: 25950237)
Cell. 2015 Jun 18;161(7):1619-32. (PMID: 26091039)
Nat Rev Genet. 2015 Aug;16(8):472-82. (PMID: 26184597)
Nucleic Acids Res. 2016 Jan 4;44(D1):D279-85. (PMID: 26673716)
Mol Cell Biol. 1989 Sep;9(9):3919-30. (PMID: 2674684)
Sci Signal. 2016 Mar 22;9(420):re3. (PMID: 27016527)
Nucleic Acids Res. 2016 Jul 8;44(W1):W242-5. (PMID: 27095192)
Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3482-91. (PMID: 27185916)
Science. 2016 Jun 24;352(6293):1542-7. (PMID: 27339980)
Nucleic Acids Res. 2017 Jan 4;45(D1):D507-D516. (PMID: 27738135)
Science. 2016 Dec 2;354(6316):. (PMID: 27934708)
Nat Rev Microbiol. 2017 Jun;15(6):323-337. (PMID: 28392566)
Trends Biochem Sci. 2017 Oct;42(10):799-811. (PMID: 28784328)
J Mol Biol. 2018 Jul 20;430(15):2237-2243. (PMID: 29258817)
Nat Rev Microbiol. 2018 May;16(5):316-328. (PMID: 29479077)
Trends Biochem Sci. 2018 May;43(5):380-394. (PMID: 29544874)
New Phytol. 2018 Jul;219(2):672-696. (PMID: 29726587)
Front Microbiol. 2018 Jun 20;9:1060. (PMID: 29973916)
Front Plant Sci. 2018 Jul 10;9:978. (PMID: 30042777)
Cell. 2018 Oct 18;175(3):809-821.e19. (PMID: 30270044)
Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7074-8. (PMID: 7688470)
Gene. 1995 Apr 14;156(1):119-22. (PMID: 7737504)
Mol Cell Biol. 1995 Jul;15(7):3917-25. (PMID: 7791797)
Yeast. 1998 Jul;14(10):953-61. (PMID: 9717241)
معلومات مُعتمدة: R00 DK099254 United States DK NIDDK NIH HHS; S10 OD025018 United States OD NIH HHS; T32 DK007257 United States DK NIDDK NIH HHS; T32 GM008203 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: HSP90; HopBF1; Pseudomonas syringae; chaperone; effector; immunity; kinase; phosphorylation
المشرفين على المادة: 0 (Arabidopsis Proteins)
0 (Bacterial Proteins)
0 (HSP90 Heat-Shock Proteins)
EC 2.7.- (Protein Kinases)
EC 3.6.1.- (Adenosine Triphosphatases)
تواريخ الأحداث: Date Created: 20190917 Date Completed: 20200608 Latest Revision: 20230715
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6754304
DOI: 10.1016/j.cell.2019.08.020
PMID: 31522888
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-4172
DOI:10.1016/j.cell.2019.08.020