دورية أكاديمية
microRNA and mRNA interactions in induced pluripotent stem cell reprogramming of lymphoblastoid cell lines.
| العنوان: | microRNA and mRNA interactions in induced pluripotent stem cell reprogramming of lymphoblastoid cell lines. |
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| المؤلفون: | Kumar S; Department of Human Genetics and South Texas Diabetes and Obesity Institute, University of Texas Rio Grande Valley School of Medicine Edinburg and Brownsville, TX, USA., Espinosa EC; Department of Human Genetics and South Texas Diabetes and Obesity Institute, University of Texas Rio Grande Valley School of Medicine Edinburg and Brownsville, TX, USA., Leandro AC; Department of Human Genetics and South Texas Diabetes and Obesity Institute, University of Texas Rio Grande Valley School of Medicine Edinburg and Brownsville, TX, USA., Curran JE; Department of Human Genetics and South Texas Diabetes and Obesity Institute, University of Texas Rio Grande Valley School of Medicine Edinburg and Brownsville, TX, USA., Blangero J; Department of Human Genetics and South Texas Diabetes and Obesity Institute, University of Texas Rio Grande Valley School of Medicine Edinburg and Brownsville, TX, USA. |
| المصدر: | American journal of stem cells [Am J Stem Cells] 2019 Aug 15; Vol. 8 (2), pp. 28-37. Date of Electronic Publication: 2019 Aug 15 (Print Publication: 2019). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: e-Century Publishing Corporation Country of Publication: United States NLM ID: 101578936 Publication Model: eCollection Cited Medium: Print ISSN: 2160-4150 (Print) Linking ISSN: 21604150 NLM ISO Abbreviation: Am J Stem Cells Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Madison, WI : e-Century Publishing Corporation 2012- |
| مستخلص: | A large number of Epstein Barr virus (EBV) immortalized lymphoblastoid cell lines (LCLs) have been generated and maintained in genetic/epidemiological studies as a perpetual source of DNA and as a surrogate in vitro cell model. Recent successes in reprograming LCLs into induced pluripotent stem cells (iPSCs) has paved the way to generate more relevant in vitro disease models using this existing bioresource. However, the latent EBV infection in the LCLs make them a unique cell type by altering expression of many cellular genes and miRNAs. These EBV induced changes in the LCL miRNome and transcriptome are reversed upon reprogramming into iPSCs, which allows a unique opportunity to better understand the miRNA and mRNA interactions that are EBV induced in LCLs and the changes that takes place during iPSC reprogramming. To identify the potential miRNA-mRNA interactions and better understand their role in regulating the cellular transitions in LCLs and their reprogrammed iPSCs, we performed a parallel genome-wide miRNA and mRNA expression analysis in six LCLs and their reprogrammed iPSCs. A total of 85 miRNAs and 5,228 mRNAs were significantly differentially expressed (DE). The target prediction of the DE miRNAs using TargetScan-Human, TarBase and miRecords databases identified 1,842 mRNA targets that were DE between LCLs and their reprogrammed iPSCs. The functional annotation, upstream regulator and gene expression analysis of the predicted DE mRNA targets suggest the role of DE miRNAs in regulating EBV induced changes in LCLs and self-renewal, pluripotency and differentiation in iPSCs. Competing Interests: None. |
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| فهرسة مساهمة: | Keywords: Human; gene expression regulation; iPSC reprogramming; lymphoblastoid cell line; mRNA; miRNA |
| تواريخ الأحداث: | Date Created: 20190917 Latest Revision: 20201001 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC6737382 |
| PMID: | 31523484 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2160-4150 |
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