دورية أكاديمية
[Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer].
| العنوان: | [Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer]. |
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| المؤلفون: | Smetannikova NA; State Research Center of Virology and Biotechnology, Koltsovo, Russia.; EpiGene LLC, Novosibirsk, Russia., Evdokimov AA; State Research Center of Virology and Biotechnology, Koltsovo, Russia.; EpiGene LLC, Novosibirsk, Russia., Netesova NA; State Research Center of Virology and Biotechnology, Koltsovo, Russia.; EpiGene LLC, Novosibirsk, Russia., Abdurashitov MA; EpiGene LLC, Novosibirsk, Russia., Akishev AG; EpiGene LLC, Novosibirsk, Russia., Dubinin EV; EpiGene LLC, Novosibirsk, Russia., Pozdnyakov PI; State Research Center of Virology and Biotechnology, Koltsovo, Russia., Vihlyanov IV; Altai Regional Oncology Center, Barnaul, Russia., Nikitin MK; Altai Regional Oncology Center, Barnaul, Russia., Topolnitsky EB; Tomsk Regional Clinical Hospital, Tomsk, Russia., Karpov AB; Seversk Biophysical Research Centre, Seversk, Russia., Kolomiets SA; Regional Clinical Oncology Center, Kemerovo, Russia., Degtyarev SK; EpiGene LLC, Novosibirsk, Russia. |
| المصدر: | Zhongguo fei ai za zhi = Chinese journal of lung cancer [Zhongguo Fei Ai Za Zhi] 2019 Sep 20; Vol. 22 (9), pp. 551-561. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Zhongguo fei ai za zhi bian ji wei yuan hui Country of Publication: China NLM ID: 101126433 Publication Model: Print Cited Medium: Internet ISSN: 1999-6187 (Electronic) Linking ISSN: 10093419 NLM ISO Abbreviation: Zhongguo Fei Ai Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2008- : Tianjin Shi : Zhongguo fei ai za zhi bian ji wei yuan hui Original Publication: Chendu Shi : Zhongguo fei ai za zhi bian ji wei yuan hui |
| مواضيع طبية MeSH: | DNA Methylation*, Lung Neoplasms/*genetics , Polymerase Chain Reaction/*methods , Regulatory Sequences, Nucleic Acid/*genetics , Tumor Suppressor Proteins/*genetics, Humans |
| مستخلص: | Hypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some downregulated genes in tissue samples of lung cancer (LC). This list includes EFEMP1, EPHA5, HOXA5, HOXA9, LHX1, MYF6, NID2, OTX1, PAX9, RARB, RASSF1A, RXRG, SIX6, SKOR1 and TERT genes. The results of DNA samples from 40 cancer and 25 normal lung tissues showed a good diagnostic potential of selected RCGY sites in regulatory regions of MYF6, SIX6, RXRG, LHX1, RASSF1A and TERT genes with relatively high sensitivity (80.0 %) and specificity (88.0 %) of LC detection in tumor DNA. |
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| فهرسة مساهمة: | Keywords: DNA methylation; Diagnostics; Epigenetics; GLAD-PCR; Lung neoplasms |
| المشرفين على المادة: | 0 (Tumor Suppressor Proteins) |
| تواريخ الأحداث: | Date Created: 20190919 Date Completed: 20200203 Latest Revision: 20200904 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC6754573 |
| DOI: | 10.3779/j.issn.1009-3419.2019.09.01 |
| PMID: | 31526458 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1999-6187 |
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| DOI: | 10.3779/j.issn.1009-3419.2019.09.01 |