دورية أكاديمية
Study of the Biomechanical and Histological Properties of the Abdominal Aorta of Diabetic Rats Exposed to Cigarette Smoke.
| العنوان: | Study of the Biomechanical and Histological Properties of the Abdominal Aorta of Diabetic Rats Exposed to Cigarette Smoke. |
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| المؤلفون: | Barão FTF; Vascular and Endovascular Division and Surgical Technique Division, Department of Surgery, University of São Paulo Medical School, São Paulo, Brazil, felipefbarao@yahoo.com.br., Barão VHP; Vascular and Endovascular Division and Surgical Technique Division, Department of Surgery, University of São Paulo Medical School, São Paulo, Brazil., Gornati VC; Vascular and Endovascular Division and Surgical Technique Division, Department of Surgery, University of São Paulo Medical School, São Paulo, Brazil., Silvestre GCR; Vascular and Endovascular Division and Surgical Technique Division, Department of Surgery, University of São Paulo Medical School, São Paulo, Brazil., Silva AQ; Vascular and Endovascular Division and Surgical Technique Division, Department of Surgery, University of São Paulo Medical School, São Paulo, Brazil., Lacchini S; Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo Medical School, São Paulo, Brazil., de Castro MM; Department of Pharmacology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil., De Luccia N; Vascular and Endovascular Division and Surgical Technique Division, Department of Surgery, University of São Paulo Medical School, São Paulo, Brazil., da Silva ES; Vascular and Endovascular Division and Surgical Technique Division, Department of Surgery, University of São Paulo Medical School, São Paulo, Brazil. |
| المصدر: | Journal of vascular research [J Vasc Res] 2019; Vol. 56 (5), pp. 255-266. Date of Electronic Publication: 2019 Sep 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: S. Karger Country of Publication: Switzerland NLM ID: 9206092 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1423-0135 (Electronic) Linking ISSN: 10181172 NLM ISO Abbreviation: J Vasc Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel ; New York : S. Karger, c1992- |
| مواضيع طبية MeSH: | Aorta, Abdominal/*pathology , Aortic Aneurysm, Abdominal/*etiology , Diabetes Mellitus, Experimental/*complications , Smoke/*adverse effects , Tobacco Products/*adverse effects, Animals ; Aorta, Abdominal/metabolism ; Aorta, Abdominal/physiopathology ; Aortic Aneurysm, Abdominal/metabolism ; Aortic Aneurysm, Abdominal/pathology ; Aortic Aneurysm, Abdominal/physiopathology ; Biomechanical Phenomena ; Collagen/metabolism ; Disease Progression ; Elastic Tissue/pathology ; Male ; Matrix Metalloproteinase 2/metabolism ; Rats, Wistar ; Regional Blood Flow ; Risk Factors ; Stress, Mechanical ; Time Factors |
| مستخلص: | Introduction: In spite of the great relevance of abdominal aortic aneurysm, its etiopathogenesis is not fully understood. The biomechanical and histological study of the aortic wall may contribute to this elucidation. Methods: Seventy-five male Wistar rats were divided into 4 groups: control (CG), smoker (SG), diabetic (DG), and diabetic + smoker (DSG). The SG and DSG rats were exposed to cigarette smoke for 30 min/day, 5 days a week. Diabetes was induced by the intravenous injection of streptozotocin. After 16 weeks, the abdominal aorta was collected for biomechanical, histological, and matrix metalloproteinase 2 (MMP-2) activity analyses. Results: The valid biomechanical tests of 52 specimens were analyzed: 11 in the CG, 10 in the DG, 16 in the SG, and 15 in the DSG. The biomechanical analysis of the fragments showed no differences between the control, DG, SG, and DSG. Collagen deposition also did not present a significant difference between the studied groups. The total count of elastic fibers was higher in diabetic rats (DG and DSG) than in the SG. The inflammatory response observed in all experimental groups was significantly more intense than in the CG. Compared to the DSG, MMP-2 activity showed a significant decrease in the DG. Conclusions: Resistance and elasticity did not present a difference between the CG and the DG, SG, and DSG. Compared to the CG, the total count of elastic fibers, fragmentation of the elastic lamina, pericellular matrix deposition, and cell loss/substitution in the tunica media showed significant alterations in the aortic walls of the DG, SG, and DSG. MMP-2 activity was lower in the DG aorta than in the DSG aorta. (© 2019 S. Karger AG, Basel.) |
| فهرسة مساهمة: | Keywords: Aorta; Biomechanics; Cigarette smoke; Diabetes mellitus; Histology; Matrix metalloproteinase 2 |
| المشرفين على المادة: | 0 (Smoke) 9007-34-5 (Collagen) EC 3.4.24.24 (Matrix Metalloproteinase 2) EC 3.4.24.24 (Mmp2 protein, rat) |
| تواريخ الأحداث: | Date Created: 20190919 Date Completed: 20200127 Latest Revision: 20200127 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1159/000502688 |
| PMID: | 31533112 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1423-0135 |
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| DOI: | 10.1159/000502688 |