دورية أكاديمية
أعرض في EDS

Assessment of Dried Blood Spots for Multi-Mycotoxin Biomarker Analysis in Pigs and Broiler Chickens.

التفاصيل البيبلوغرافية
العنوان: Assessment of Dried Blood Spots for Multi-Mycotoxin Biomarker Analysis in Pigs and Broiler Chickens.
المؤلفون: Lauwers M; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium. marianne.lauwers@ugent.be.; Innovad Global, Postbaan 69, 2910 Essen, Belgium. marianne.lauwers@ugent.be., Croubels S; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium. siska.croubels@ugent.be., De Baere S; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium. siegrid.debaere@ugent.be., Sevastiyanova M; Innovad Global, Postbaan 69, 2910 Essen, Belgium. m.sevastiyanova@innovad-global.com., Romera Sierra EM; Innovad Global, Postbaan 69, 2910 Essen, Belgium. e.romera@innovad-global.com., Letor B; Innovad Global, Postbaan 69, 2910 Essen, Belgium. b.letor@innovad-global.com., Gougoulias C; Innovad Global, Postbaan 69, 2910 Essen, Belgium. C.Gougoulias@innovad-global.com., Devreese M; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium. mathias.devreese@ugent.be.
المصدر: Toxins [Toxins (Basel)] 2019 Sep 18; Vol. 11 (9). Date of Electronic Publication: 2019 Sep 18.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101530765 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6651 (Electronic) Linking ISSN: 20726651 NLM ISO Abbreviation: Toxins (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مواضيع طبية MeSH: Mycotoxins/*blood, Animals ; Biomarkers/blood ; Chickens ; Chromatography, Liquid ; Dried Blood Spot Testing ; Female ; Hematocrit ; Mycotoxins/pharmacokinetics ; Mycotoxins/toxicity ; Swine ; Tandem Mass Spectrometry
مستخلص: Dried blood spots (DBSs), a micro-sampling technique whereby a drop of blood is collected on filter paper has multiple advantages over conventional blood sampling regarding the sampling itself, as well as transportation and storage. This is the first paper describing the development and validation of a method for the determination of 23 mycotoxins and phase I metabolites in DBSs from pigs and broiler chickens using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The targeted mycotoxins belong to groups for which the occurrence in feed is regulated by the European Union, namely, aflatoxins, ochratoxin A and several Fusarium mycotoxins, and to two groups of unregulated mycotoxins, namely Alternaria mycotoxins and Fusarium mycotoxins (enniatins and beauvericin). The impact of blood haematocrit, DBS sampling volume and size of the analysed DBS disk on the validation results was assessed. No effects of variation in size of the analysed disk, haematocrit and spotted blood volume were observed for most mycotoxins, except for the aflatoxins and β-zearalanol (BZAL) at the lowest haematocrit (26%) level and for the enniatins (ENNs) at the lowest volume (40 µL). The developed method was transferred to an LC-high resolution mass spectrometry instrument to determine phase II metabolites. Then, the DBS technique was applied in a proof-of-concept toxicokinetic study including a comparison with LC-MS/MS data from plasma obtained with conventional venous blood sampling. A strong correlation ( r > 0.947) was observed between plasma and DBS concentrations. Finally, DBSs were also applied in a pilot exposure assessment study to test their applicability under field conditions.
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فهرسة مساهمة: Keywords: biomarkers for exposure; broiler chickens; dried blood spots; exposure assessment; multi-mycotoxin analysis; pigs; toxicokinetic study
المشرفين على المادة: 0 (Biomarkers)
0 (Mycotoxins)
تواريخ الأحداث: Date Created: 20190922 Date Completed: 20201002 Latest Revision: 20201002
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6784198
DOI: 10.3390/toxins11090541
PMID: 31540490
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6651
DOI:10.3390/toxins11090541