دورية أكاديمية
أعرض في EDS

Dendritic Cells Expressing MyD88 Molecule Are Necessary and Sufficient for CpG-Mediated Inhibition of IgE Production In Vivo.

التفاصيل البيبلوغرافية
العنوان: Dendritic Cells Expressing MyD88 Molecule Are Necessary and Sufficient for CpG-Mediated Inhibition of IgE Production In Vivo.
المؤلفون: Alberca Custodio RW; Institute of Biomedical Sciences, Department of Immunology, University of Sao Paulo, Sao Paulo 05508-000, Brazil. Ricardowesley@gmail.com., Mirotti L; Institute of Biomedical Sciences, Department of Immunology, University of Sao Paulo, Sao Paulo 05508-000, Brazil. lmirotti@gmail.com., Gomes E; Institute of Biomedical Sciences, Department of Immunology, University of Sao Paulo, Sao Paulo 05508-000, Brazil. melloeag@gmail.com., Nunes FPB; Institute of Biomedical Sciences, Department of Immunology, University of Sao Paulo, Sao Paulo 05508-000, Brazil., S Vieira R; Institute of Biomedical Sciences, Department of Immunology, University of Sao Paulo, Sao Paulo 05508-000, Brazil. raqueldesouzavieira@outlook.com., Graça L; Institute of Molecular Medicine, University of Lisbon, 1649-028 Lisbon, Portugal. lgraca@medicina.ulisboa.pt., R Almeida R; Institute of Biomedical Sciences, Department of Immunology, University of Sao Paulo, Sao Paulo 05508-000, Brazil. rafaelbio13@yahoo.com.br., S Câmara NO; Institute of Biomedical Sciences, Department of Immunology, University of Sao Paulo, Sao Paulo 05508-000, Brazil. niels@icb.usp.br., Russo M; Institute of Biomedical Sciences, Department of Immunology, University of Sao Paulo, Sao Paulo 05508-000, Brazil. momrusso@usp.br.
المصدر: Cells [Cells] 2019 Sep 28; Vol. 8 (10). Date of Electronic Publication: 2019 Sep 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101600052 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4409 (Electronic) Linking ISSN: 20734409 NLM ISO Abbreviation: Cells Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Adjuvants, Immunologic/*administration & dosage , Dendritic Cells/*immunology , Immunoglobulin E/*metabolism , Myeloid Differentiation Factor 88/*metabolism , Oligodeoxyribonucleotides/*administration & dosage , Ovalbumin/*adverse effects , Respiratory Hypersensitivity/*drug therapy, Adjuvants, Immunologic/pharmacology ; Animals ; B-Lymphocytes/drug effects ; B-Lymphocytes/immunology ; B-Lymphocytes/metabolism ; Dendritic Cells/drug effects ; Dendritic Cells/metabolism ; Disease Models, Animal ; Female ; Gene Expression Regulation/drug effects ; Humans ; Immunoglobulin E/drug effects ; Mice ; Myeloid Differentiation Factor 88/genetics ; Oligodeoxyribonucleotides/pharmacology ; Respiratory Hypersensitivity/chemically induced ; Respiratory Hypersensitivity/metabolism ; Signal Transduction/drug effects
مستخلص: Elevated levels of immunoglobulin E (IgE) are associated with allergies and other immunological disorders. Sensitization with alum adjuvant favours IgE production while CpG-ODN adjuvant, a synthetic toll-like receptor 9 (TLR9) agonist, inhibits it. The cellular mechanisms underlying in vivo TLR regulation of immunoglobulin production, specially IgE, are still controversial. Specifically, TLR-mediated IgE regulation in vivo is not yet known. In this study we showed that augmented levels of IgE induced by sensitizations to OVA with or without alum adjuvant or with OVA-pulsed dendritic cells (DCs) were inhibited by co-administration of CpG. Notably, CpG-mediated suppression of IgE production required MyD88 -expression on DCs but not on B-cells. This finding contrasts with previous in vitro studies reporting regulation of IgE by a direct action of CpG on B cells via MyD88 pathway. In addition, we showed that CpG also inhibited IgE production in a MyD88 -dependent manner when sensitization was performed with OVA-pulsed DCs. Finally, CpG signalling through MyD88 pathway was also necessary and sufficient to prevent anaphylactic antibody production involved in active cutaneous anaphylaxis.
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فهرسة مساهمة: Keywords: IgE; IgG2c; allergy; anaphylaxis; dendritic cells
المشرفين على المادة: 0 (Adjuvants, Immunologic)
0 (CPG-oligonucleotide)
0 (Myd88 protein, mouse)
0 (Myeloid Differentiation Factor 88)
0 (Oligodeoxyribonucleotides)
37341-29-0 (Immunoglobulin E)
9006-59-1 (Ovalbumin)
تواريخ الأحداث: Date Created: 20191002 Date Completed: 20200615 Latest Revision: 20200615
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6829343
DOI: 10.3390/cells8101165
PMID: 31569343
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4409
DOI:10.3390/cells8101165