دورية أكاديمية
أعرض في EDS

Purification and Characterization of Anabaena flos-aquae Phenylalanine Ammonia-Lyase as a Novel Approach for Myristicin Biotransformation.

التفاصيل البيبلوغرافية
العنوان: Purification and Characterization of Anabaena flos-aquae Phenylalanine Ammonia-Lyase as a Novel Approach for Myristicin Biotransformation.
المؤلفون: Arafa AM; Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt., Abdel-Ghany AE; Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt., El-Dahmy SI; Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt., Abdelaziz S; Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt., El-Ayouty Y; Enzymology and Fungal Biotechnology Lab (EFBL), Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt., El-Sayed ASA; Enzymology and Fungal Biotechnology Lab (EFBL), Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.
المصدر: Journal of microbiology and biotechnology [J Microbiol Biotechnol] 2020 Apr 28; Vol. 30 (4), pp. 622-632.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Korean Society for Microbiology and Biotechnology Country of Publication: Korea (South) NLM ID: 9431852 Publication Model: Print Cited Medium: Internet ISSN: 1738-8872 (Electronic) Linking ISSN: 10177825 NLM ISO Abbreviation: J Microbiol Biotechnol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2002- : Seoul : Korean Society for Microbiology and Biotechnology
Original Publication: Seoul : Korean Society for Applied Microbiology, 1991-2001.
مواضيع طبية MeSH: Benzyl Compounds/*metabolism , Dioxolanes/*metabolism , Dolichospermum flos-aquae/*enzymology , Phenylalanine Ammonia-Lyase/*isolation & purification , Phenylalanine Ammonia-Lyase/*metabolism , Pyrogallol/*analogs & derivatives, 3,4-Methylenedioxyamphetamine/analogs & derivatives ; 3,4-Methylenedioxyamphetamine/metabolism ; Allylbenzene Derivatives ; Bacterial Proteins/chemistry ; Bacterial Proteins/isolation & purification ; Bacterial Proteins/metabolism ; Biotransformation ; Hydrogen-Ion Concentration ; Molecular Structure ; Molecular Weight ; Phenylalanine Ammonia-Lyase/chemistry ; Pyrogallol/metabolism ; Spirulina/enzymology ; Substrate Specificity ; Temperature
مستخلص: Phenylalanine ammonia-lyase (PAL) catalyzes the reversible deamination of phenylalanine to cinnamic acid and ammonia. Algae have been considered as biofactories for PAL production, however, biochemical characterization of PAL and its potency for myristicin biotransformation into MMDA (3-methoxy-4, 5-methylenedioxyamphetamine) has not been studied yet. Thus, PAL from Anabaena flos-aquae and Spirulina platensis has been purified, comparatively characterized and its affinity to transform myristicin was assessed. The specific activity of purified PAL from S. platensis (73.9 μmol/mg/min) and A. flos-aquae (30.5 μmol/mg/min) was increased by about 2.9 and 2.4 folds by gel-filtration comparing to their corresponding crude enzymes. Under denaturing-PAGE, a single proteineous band with a molecular mass of 64 kDa appeared for A. flos-aquae and S. platensis PAL. The biochemical properties of the purified PAL from both algal isolates were determined comparatively. The optimum temperature of S. platensis and A. flos-aquae PAL for forward or reverse activity was reported at 30°C, while the optimum pH for PAL enzyme isolated from A. flos-aquae was 8.9 for forward and reverse activities, and S. platensis PAL had maximum activities at pH 8.9 and 8 for forward and reverse reactions, respectively. Luckily, the purified PALs have the affinity to hydroaminate the myristicin to MMDA successfully in one step. Furthermore, a successful method for synthesis of MMDA from myristicin in two steps was also established. Gas chromatography-mass spectrometry (GC-MS) analysis was conducted to track the product formation.
References: Protein Eng Des Sel. 2010 Dec;23(12):929-33. (PMID: 21036782)
Addiction. 2010 Aug;105(8):1355-61. (PMID: 20653618)
Biomed Res Int. 2017;2017:1272193. (PMID: 28280725)
BMC Microbiol. 2004 Mar 18;4:11. (PMID: 15113456)
Chem Biol. 2006 Dec;13(12):1327-38. (PMID: 17185228)
J Insect Sci. 2015 Jul 01;15:. (PMID: 26136499)
Z Naturforsch C Biosci. 1976 Nov-Dec;31(11-12):693-9. (PMID: 138288)
Pharmacology. 1973;9(5):312-6. (PMID: 4737998)
J Biol Chem. 1951 Nov;193(1):265-75. (PMID: 14907713)
Biochemistry. 1968 May;7(5):1904-14. (PMID: 5655435)
PLoS One. 2014 Jan 27;9(1):e85943. (PMID: 24475062)
Bioorg Med Chem. 2014 Oct 15;22(20):5555-7. (PMID: 25037641)
Sci Rep. 2017 Oct 20;7(1):13691. (PMID: 29057979)
Drug Test Anal. 2018 Jan;10(1):72-80. (PMID: 28851034)
Enzyme Res. 2013;2013:670702. (PMID: 24159382)
Molecules. 2011;16(8):7132-42. (PMID: 21991618)
Nature. 1970 Aug 15;227(5259):680-5. (PMID: 5432063)
Mycobiology. 2011 Dec;39(4):257-65. (PMID: 22783113)
Enzyme Microb Technol. 2019 May;124:41-53. (PMID: 30797478)
Crit Rev Biotechnol. 2014 Sep;34(3):258-68. (PMID: 23688066)
Biotechnol Prog. 2015 Mar-Apr;31(2):396-405. (PMID: 25582958)
Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):1811-3. (PMID: 10051548)
J Adv Res. 2015 Jan;6(1):17-33. (PMID: 25685541)
Biochemistry. 2007 Jan 30;46(4):1004-12. (PMID: 17240984)
Biotechnol Lett. 2013 May;35(5):751-6. (PMID: 23338700)
Enzyme Microb Technol. 2014 Jan 10;54:59-69. (PMID: 24267569)
Pharmacology. 1973;10(1):12-8. (PMID: 4751535)
Mol Biol Rep. 2011 Aug;38(6):3741-50. (PMID: 21104014)
Biotechnol Appl Biochem. 2015 Jul-Aug;62(4):514-22. (PMID: 25273833)
Front Microbiol. 2016 Mar 08;7:281. (PMID: 27014206)
Enzyme Microb Technol. 2012 Sep 10;51(4):200-10. (PMID: 22883554)
J Microbiol Biotechnol. 2013 Apr;23(4):499-510. (PMID: 23568204)
Enzyme Microb Technol. 2015 Dec;81:31-46. (PMID: 26453470)
J Biol Chem. 1976 Aug 10;251(15):4646-50. (PMID: 985816)
Molecules. 2014 Jan 27;19(2):1459-80. (PMID: 24473208)
J Appl Microbiol. 2011 Jul;111(1):54-69. (PMID: 21466637)
J Biol Chem. 1961 Oct;236:2692-8. (PMID: 14458851)
Top Catal. 2018;61(3):288-295. (PMID: 30956511)
Mol Ther. 2004 Aug;10(2):220-4. (PMID: 15294168)
Bacteriol Rev. 1971 Jun;35(2):171-205. (PMID: 4998365)
Drug Alcohol Depend. 1978 May;3(3):153-63. (PMID: 668489)
Appl Biochem Biotechnol. 2017 Apr;181(4):1513-1532. (PMID: 27796875)
Mol Genet Metab. 2011 Nov;104(3):249-54. (PMID: 21803624)
Appl Microbiol Biotechnol. 2016 Jul;100(14):6103-6117. (PMID: 27209039)
J Biotechnol. 2017 Sep 20;258:148-157. (PMID: 28392421)
Arch Pharm Res. 2014 Oct 18;:. (PMID: 25322968)
J Agric Food Chem. 2010 Oct 27;58(20):10911-7. (PMID: 20863129)
Biotechnol Lett. 2010 Dec;32(12):1877-81. (PMID: 20676917)
Z Naturforsch C J Biosci. 2005 May-Jun;60(5-6):411-4. (PMID: 16042341)
Mol Cells. 1997 Dec 31;7(6):715-20. (PMID: 9509410)
Appl Environ Microbiol. 1981 Nov;42(5):773-8. (PMID: 16345879)
Insect Biochem Mol Biol. 2008 Jun;38(6):645-51. (PMID: 18510976)
Nature. 1964 Mar 14;201:1120-1. (PMID: 14152788)
J Chromatogr B Biomed Sci Appl. 1998 Feb 13;705(2):367-72. (PMID: 9521577)
Arch Gen Psychiatry. 1971 Jan;24(1):50-5. (PMID: 4923215)
Biochem Cell Biol. 2007 Jun;85(3):273-82. (PMID: 17612622)
Pharmacogn Rev. 2010 Jul;4(8):179-84. (PMID: 22228959)
Prague Med Rep. 2005;106(1):27-38. (PMID: 16007907)
فهرسة مساهمة: Keywords: MMDA; Phenylalanine ammonia-Lyase; myristicin; properties; purification
المشرفين على المادة: 0 (Allylbenzene Derivatives)
0 (Bacterial Proteins)
0 (Benzyl Compounds)
0 (Dioxolanes)
01Y4A2QXY0 (Pyrogallol)
04PD6CT78W (myristicin)
13674-05-0 (MMDA)
4764-17-4 (3,4-Methylenedioxyamphetamine)
EC 4.3.1.24 (Phenylalanine Ammonia-Lyase)
SCR Organism: Arthrospira platensis
تواريخ الأحداث: Date Created: 20191004 Date Completed: 20201229 Latest Revision: 20221214
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9728195
DOI: 10.4014/jmb.1908.08009
PMID: 31581382
قاعدة البيانات: MEDLINE
الوصف
تدمد:1738-8872
DOI:10.4014/jmb.1908.08009