دورية أكاديمية
أعرض في EDS

Microbial Colonization of Pneumatic Tourniquets in the Orthopedic Operating Room.

التفاصيل البيبلوغرافية
العنوان: Microbial Colonization of Pneumatic Tourniquets in the Orthopedic Operating Room.
المؤلفون: Mufarrih SH; Orthopedic Surgery, Aga Khan University, Karachi, PAK., Qureshi NQ; Cardiology, Aga Khan University, Karachi, PAK., Rashid RH; Orthopedic Surgery, Aga Khan University, Karachi, PAK., Ahmed B; Medicine, Aga Khan University, Karachi, PAK., Irfan S; Microbiology, Aga Khan University, Karachi, PAK., Zubairi AJ; Orthopedic Surgery, Aga Khan University, Karachi, PAK., Noordin S; Orthopedic Surgery, Aga Khan University, Karachi, PAK.
المصدر: Cureus [Cureus] 2019 Aug 02; Vol. 11 (8), pp. e5308. Date of Electronic Publication: 2019 Aug 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Cureus, Inc Country of Publication: United States NLM ID: 101596737 Publication Model: Electronic Cited Medium: Print ISSN: 2168-8184 (Print) Linking ISSN: 21688184 NLM ISO Abbreviation: Cureus Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Palo Alto, CA : Cureus, Inc.
مستخلص: Background The rate of surgical site infections following orthopedic procedures is approximately 2% globally. Potential sources of contamination in the operating room include pneumatic tourniquets, blood pressure cuffs, and stethoscopes, among others. Our study aims to investigate microbial colonization on reusable pneumatic tourniquets stored and used in the orthopedic department of our institution and evaluate the efficacy of the cleaning protocols employed. Methods Over a course of two weeks, 26 samples were obtained. A total of 14 pneumatic tourniquets were sampled preoperatively on Monday morning following the weekly cleaning protocol of soaking the tourniquets in sodium hypochlorite for 30 minutes while 12 tourniquets were cultured immediately following the postoperative cleaning protocol of wiping the tourniquet clean with a cloth soaked in sodium hypochlorite. Samples were cultured on MacConkey and sheep blood agar and incubated at 37-degrees centigrade for a total of 48 hours. Organisms were identified and colony count was documented. The analysis was performed using the Fisher Exact test on SPSS v23 (IBM Corp., Armonk, NY, US). Results All 14 samples obtained after being soaked in sodium hypochlorite for 30 minutes cultured negative. However, four out of 12 (33%) samples obtained after simply wiping the pneumatic tourniquet with a cloth soaked in sodium hypochlorite cultured coagulase-negative Staphylococci. The difference between the two was significant (p=0.002). Conclusion Postoperative tourniquets, wiped with a cloth soaked in sodium hypochlorite and ready to be used on the next patient, were found to be contaminated with coagulase-negative Staphylococcus. This species is notorious for causing surgical site infections following implant-related surgeries potentially through direct inoculation and cross-infections intraoperatively and in storage. Efforts to identify the relationship with postoperative surgical site infections need to be made to suggest more aggressive cleaning protocols.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright © 2019, Mufarrih et al.)
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فهرسة مساهمة: Keywords: infection; joint arthroplasty; operating room; surgical site infection; tourniquet
تواريخ الأحداث: Date Created: 20191009 Latest Revision: 20200930
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6773449
DOI: 10.7759/cureus.5308
PMID: 31592363
قاعدة البيانات: MEDLINE
الوصف
تدمد:2168-8184
DOI:10.7759/cureus.5308