دورية أكاديمية
Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus.
| العنوان: | Combination of quercetin and ivermectin: In vitro and in vivo effects against Haemonchus contortus. |
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| المؤلفون: | Borges DGL; Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Avenida Senador Filinto Müller 2443, Ipiranga, Caixa Postal 549, CEP: 79074-460, Campo Grande, MS, Brazil. Electronic address: dyego.borges@ufms.br., de Araújo MA; Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Avenida Senador Filinto Müller 2443, Ipiranga, Caixa Postal 549, CEP: 79074-460, Campo Grande, MS, Brazil., Carollo CA; Instituto de Biociências, Universidade Federal de Mato Grosso do Sul, Brazil., Carollo ARH; Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição, Universidade Federal de Mato Grosso do Sul, Brazil., Lifschitz A; Laboratório de Farmacologia, Centro de Investigação Veterinária de Tandil, (CIVETAN), (UNCPBA- CICPBA-CONICET), Faculdade de Ciências Veterinárias, Universidade Nacional do Centro, Tandil, Argentina., Conde MH; Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Avenida Senador Filinto Müller 2443, Ipiranga, Caixa Postal 549, CEP: 79074-460, Campo Grande, MS, Brazil., de Freitas MG; Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Avenida Senador Filinto Müller 2443, Ipiranga, Caixa Postal 549, CEP: 79074-460, Campo Grande, MS, Brazil., Santos Freire ZD; Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Avenida Senador Filinto Müller 2443, Ipiranga, Caixa Postal 549, CEP: 79074-460, Campo Grande, MS, Brazil., Tutija JF; Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Avenida Senador Filinto Müller 2443, Ipiranga, Caixa Postal 549, CEP: 79074-460, Campo Grande, MS, Brazil., Nakatani MTM; Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Avenida Senador Filinto Müller 2443, Ipiranga, Caixa Postal 549, CEP: 79074-460, Campo Grande, MS, Brazil., Borges FA; Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Avenida Senador Filinto Müller 2443, Ipiranga, Caixa Postal 549, CEP: 79074-460, Campo Grande, MS, Brazil. |
| المصدر: | Acta tropica [Acta Trop] 2020 Jan; Vol. 201, pp. 105213. Date of Electronic Publication: 2019 Oct 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6254 (Electronic) Linking ISSN: 0001706X NLM ISO Abbreviation: Acta Trop Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Basel. |
| مواضيع طبية MeSH: | Anthelmintics/*therapeutic use , Drug Resistance/*drug effects , Haemonchiasis/*drug therapy , Haemonchus/*drug effects , Ivermectin/*therapeutic use , Larva/*drug effects , Quercetin/*therapeutic use , Sheep Diseases/*drug therapy, Animals ; Female ; Male ; Parasite Egg Count ; Sheep/parasitology ; Sheep Diseases/parasitology |
| مستخلص: | The aim of the present study was to evaluate the in vitro effect of quercetin combined with ivermectin (IVM) on Haemonchus contortus larvae and adults with different resistance profiles and demonstrate the in vivo anthelmintic action of this combination when used in sheep naturally infected. The effect of combination was evaluated based on the analysis of the mean effective concentration (EC50) obtained for larvae using the larval migration inhibition test and for adults using the motility test on females. The tests with larvae and adults were conducted using isolates with different degrees of susceptibility to IVM (sensitive, intermediate and highly resistant). The in vivo effect was evaluated based on the reduction in the egg count (FEC) and reduction in the count of adult helminths recovered after parasitological necropsy. Using the combination of quercetin with IVM, it was observed that in larvae, quercetin did not significantly reduce the EC50 for IVM in the sensitive and highly resistant isolates, but led to a significant reduction in the EC50 for IVM in the intermediate isolate. In adults, quercetin did not significantly reduce the EC50 for IVM in any of the isolates. No significant effect of the combination was found regarding the reduction in FEC or total count of parasites. The results of the in vitro and in vivo tests performed in the present study on quercetin activity underscore the importance of evaluating resistance-reversing agents among different stages of parasite development as well as among isolates with different resistance profiles. The action of quercetin combined with IVM on the motility of H. contortus larvae and adults was influenced by the degree of resistance and development stage of the parasite. The combination was effective only on intermediate resistant larvae. No action of the combination against adults was found. Moreover, this combination, when administered through the intra-abomasal route, was not effective at reducing the FEC and parasite load of naturally infected sheep. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Anthelmintic resistance; Flavonoids; Modulation; Parasites; Ruminants |
| المشرفين على المادة: | 0 (Anthelmintics) 70288-86-7 (Ivermectin) 9IKM0I5T1E (Quercetin) |
| تواريخ الأحداث: | Date Created: 20191011 Date Completed: 20200213 Latest Revision: 20200213 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.actatropica.2019.105213 |
| PMID: | 31600523 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6254 |
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| DOI: | 10.1016/j.actatropica.2019.105213 |