دورية أكاديمية
Immune complex hyperlipidemia induced by an apolipoprotein-reactive immunoglobulin A paraprotein from a patient with multiple myeloma. Characterization of this immunoglobulin.
| العنوان: | Immune complex hyperlipidemia induced by an apolipoprotein-reactive immunoglobulin A paraprotein from a patient with multiple myeloma. Characterization of this immunoglobulin. |
|---|---|
| المؤلفون: | Kilgore LL, Patterson BW, Parenti DM, Fisher WR |
| المصدر: | The Journal of clinical investigation [J Clin Invest] 1985 Jul; Vol. 76 (1), pp. 225-32. |
| نوع المنشور: | Case Reports; Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Clinical Investigation Country of Publication: United States NLM ID: 7802877 Publication Model: Print Cited Medium: Print ISSN: 0021-9738 (Print) Linking ISSN: 00219738 NLM ISO Abbreviation: J Clin Invest Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1999- : Ann Arbor, MI : American Society for Clinical Investigation Original Publication: New Haven [etc.] American Society for Clinical Investigation. |
| مواضيع طبية MeSH: | Hyperlipidemias/*immunology , Immune Complex Diseases/*blood , Multiple Myeloma/*blood, Aged ; Antigen-Antibody Complex ; Apolipoproteins B/immunology ; Apolipoproteins E/immunology ; Humans ; Immunoglobulin A/immunology ; Lipoproteins, LDL/immunology ; Lipoproteins, VLDL/immunology ; Male ; Multiple Myeloma/immunology ; Paraproteinemias/blood ; Paraproteins/immunology |
| مستخلص: | An antibodylike paraprotein has been isolated from a patient with multiple myeloma and autoimmune hyperlipoproteinemia. The paraprotein bound to apolipoprotein B (apo B)-containing lipoproteins that formed macromolecular aggregates, and globules thought to be aggregated complexes of lipoproteins and reactive immunoglobulins were observed circulating within the retinal blood vessels of this patient. This binding specificity permitted purification of the paraprotein from both the agglutinated immune complexes and from the plasma. The protein is an IgA, kappa-immunoglobulin which exists primarily in a polymeric state. Capillary immunoprecipitation demonstrated reactivity with very low density lipoproteins (VLDL) and low density proteins (LDL), but not with high density lipoproteins (HDL). Delipidated apo B and apo E, but not apo A or apo C, formed precipitates with this immunoglobulin. In using a radioimmunoassay format, the affinity of the immunoglobulin was greatest for VLDL and decreases sequentially for intermediate density lipoproteins and LDL. No binding occurred with a dispersion of LDL lipids or with HDL. Deglycosylation did not change the binding to LDL. The apolipoproteins B and E bound with similar affinity, but no binding occurred with apo A-I or apo A-II. Weak binding appeared to occur with apo C. This paraprotein immunoprecipitated apo B-containing lipoproteins from all classes of vertebrates tested. Displacement of the lipids of LDL by Triton X-100 resulted in the formation of an apo B-Triton complex which, however, did not bind to the immunoglobulin; apparently the binding site on apo B was lost. Upon enzymatic digestion with the IgA-specific protease from Streptococcus sanguis the immunoglobulin was cleaved into Fc and Fab fragments, and the binding of LDL occurred only with the latter, consistent with the behavior of an immunoglobulin. The immunoreactivity of this paraprotein with apo B and apo E raises the interesting possibility that it may be binding to a site on these apolipoproteins which is reactive with the apo B, E receptor of the plasma membrane, a site which is conserved throughout the vertebrate phylum. |
| References: | Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43. (PMID: 4971842) Methods Biochem Anal. 1954;1:459-78. (PMID: 13193538) J Lab Clin Med. 1972 May;79(5):731-44. (PMID: 5018738) J Biol Chem. 1972 Jun 10;247(11):3376-81. (PMID: 4337851) J Biol Chem. 1973 Jul 25;248(14):4941-6. (PMID: 4352183) Biomedicine. 1973 Mar;18(2):145-51. (PMID: 4199987) J Lab Clin Med. 1976 Nov;88(5):769-79. (PMID: 978040) Atherosclerosis. 1977 Apr;26(4):405-18. (PMID: 67846) Proc Natl Acad Sci U S A. 1977 Nov;74(11):5150-4. (PMID: 200946) Arch Dermatol. 1978 Mar;114(3):425-31. (PMID: 343719) J Biochem. 1978 Mar;83(3):755-9. (PMID: 205534) Anal Biochem. 1978 Jun 15;87(1):206-10. (PMID: 98070) Klin Wochenschr. 1975 Apr 15;53(8):353-61. (PMID: 80469) Adv Exp Med Biol. 1978;107:489-95. (PMID: 33542) Biochim Biophys Acta. 1979 Mar 29;572(3):537-40. (PMID: 219898) Biomedicine. 1979 Jun;31(3):80-2. (PMID: 226198) Clin Exp Immunol. 1980 Feb;39(2):455-60. (PMID: 6771077) Proc Natl Acad Sci U S A. 1980 Jul;77(7):3806-10. (PMID: 6933436) J Immunol. 1981 Jul;127(1):316-9. (PMID: 7240746) Metabolism. 1981 Sep;30(9):927-35. (PMID: 6455584) N Engl J Med. 1982 Jul 8;307(2):79-83. (PMID: 6283345) Biochemistry. 1982 Aug 31;21(18):4503-11. (PMID: 7126555) J Lipid Res. 1984 Aug;25(8):763-9. (PMID: 6491521) Anal Biochem. 1985 Feb 15;145(1):113-9. (PMID: 4003754) Biochemistry. 1971 Apr 27;10(9):1622-9. (PMID: 5580673) |
| معلومات مُعتمدة: | HL-10316 United States HL NHLBI NIH HHS; HL-29394 United States HL NHLBI NIH HHS |
| المشرفين على المادة: | 0 (Antigen-Antibody Complex) 0 (Apolipoproteins B) 0 (Apolipoproteins E) 0 (Immunoglobulin A) 0 (Lipoproteins, LDL) 0 (Lipoproteins, VLDL) 0 (Paraproteins) |
| تواريخ الأحداث: | Date Created: 19850701 Date Completed: 19850828 Latest Revision: 20181113 |
| رمز التحديث: | 20240627 |
| مُعرف محوري في PubMed: | PMC423752 |
| DOI: | 10.1172/JCI111951 |
| PMID: | 3160725 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0021-9738 |
|---|---|
| DOI: | 10.1172/JCI111951 |