دورية أكاديمية
Trypanosoma rangeli 28Sβ Ribosomal Gene Allows Intra and Interspecific Molecular Differentiation.
| العنوان: | Trypanosoma rangeli 28Sβ Ribosomal Gene Allows Intra and Interspecific Molecular Differentiation. |
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| المؤلفون: | Santos RERS; Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto (FMRP), Universidade de São Paulo (USP), Ribeirão Preto, Brasil.; Instituto de Ciências Biológicas e Naturais (ICBN), Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, Brasil., Naves LL; Instituto de Ciências Biológicas e Naturais (ICBN), Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, Brasil., Fajardo EF; Instituto de Ciências Biológicas e Naturais (ICBN), Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, Brasil., Ramirez LE; Instituto de Ciências Biológicas e Naturais (ICBN), Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, Brasil., Lages-Silva E; Instituto de Ciências Biológicas e Naturais (ICBN), Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, Brasil., Pedrosa AL; Instituto de Ciências Biológicas e Naturais (ICBN), Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, Brasil., Ferreira KAM; Centro de Educação Profissional (CEFORES), Universidade Federal do Triângulo Mineiro (UFTM), Uberaba, Brasil. |
| المصدر: | Vector borne and zoonotic diseases (Larchmont, N.Y.) [Vector Borne Zoonotic Dis] 2020 Feb; Vol. 20 (2), pp. 117-124. Date of Electronic Publication: 2019 Oct 22. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 100965525 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1557-7759 (Electronic) Linking ISSN: 15303667 NLM ISO Abbreviation: Vector Borne Zoonotic Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., [2001- |
| مواضيع طبية MeSH: | DNA, Ribosomal*, Trypanosoma rangeli/*classification , Trypanosoma rangeli/*genetics, DNA, Protozoan/genetics ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Sequence Analysis, DNA ; Species Specificity ; Trypanosoma/classification ; Trypanosoma/genetics ; Trypanosoma cruzi/genetics |
| مستخلص: | Trypanosoma rangeli is an avirulent flagellate protozoan that could mislead correct diagnosis of Trypanosoma cruzi infection, the causative agent of Chagas' disease, given their high similarity. Besides, T. rangeli presents two genetic groups, whose differentiation is achieved mainly by molecular approaches. In this context, ribosomal DNA (rDNA) is a useful target for intra and interspecific molecular differentiation. Analyzing the rDNA of T. rangeli and comparison with other trypanosomatid species, two highly divergent regions (Trβ1 and Trβ2) within the 28Sβ gene were found. Those regions were amplified and sequenced in KP1(+) and KP1(-) strains of T. rangeli , revealing group-specific polymorphisms useful for intraspecific distinction through restriction fragment length polymorphism technique. Also, amplification of Trβ1 allowed differentiation between T. rangeli and T. cruzi. Trβ2 predicted restriction length profile, allowed differentiation between T. rangeli , T. cruzi , Trypanosoma brucei , and Leishmania braziliensis , increasing the use of Trβ1 and Trβ2 beyond a molecular approach for T. rangeli genotyping, but also as a useful target for trypanosomatid classification. |
| فهرسة مساهمة: | Keywords: Trypanosoma rangeli; interspecific differentiation; intraspecific differentiation; molecular characterization; ribosomal DNA |
| المشرفين على المادة: | 0 (DNA, Protozoan) 0 (DNA, Ribosomal) |
| تواريخ الأحداث: | Date Created: 20191023 Date Completed: 20201215 Latest Revision: 20201215 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1089/vbz.2019.2496 |
| PMID: | 31638479 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1557-7759 |
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| DOI: | 10.1089/vbz.2019.2496 |