دورية أكاديمية
Bacterial biodiversity drives the evolution of CRISPR-based phage resistance.
| العنوان: | Bacterial biodiversity drives the evolution of CRISPR-based phage resistance. |
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| المؤلفون: | Alseth EO; Environment and Sustainability Institute, Biosciences, University of Exeter, Penryn Campus, Penryn, UK. eao210@exeter.ac.uk., Pursey E; Environment and Sustainability Institute, Biosciences, University of Exeter, Penryn Campus, Penryn, UK., Luján AM; IRNASUS, CONICET, Facultad de Ciencias Químicas, Universidad Católica de Córdoba, Córdoba, Argentina., McLeod I; Environment and Sustainability Institute, Biosciences, University of Exeter, Penryn Campus, Penryn, UK., Rollie C; Environment and Sustainability Institute, Biosciences, University of Exeter, Penryn Campus, Penryn, UK., Westra ER; Environment and Sustainability Institute, Biosciences, University of Exeter, Penryn Campus, Penryn, UK. e.r.westra@exeter.ac.uk. |
| المصدر: | Nature [Nature] 2019 Oct; Vol. 574 (7779), pp. 549-552. Date of Electronic Publication: 2019 Oct 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4687 (Electronic) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Basingstoke : Nature Publishing Group Original Publication: London, Macmillan Journals ltd. |
| مواضيع طبية MeSH: | Biodiversity* , Evolution, Molecular*, Bacteriophages/*genetics , Bacteriophages/*immunology , CRISPR-Cas Systems/*genetics , Pseudomonas aeruginosa/*immunology , Pseudomonas aeruginosa/*virology, Bacteriophages/pathogenicity ; CRISPR-Cas Systems/immunology ; Pseudomonas aeruginosa/genetics ; Pseudomonas aeruginosa/pathogenicity ; Receptors, Virus/metabolism |
| مستخلص: | About half of all bacteria carry genes for CRISPR-Cas adaptive immune systems 1 , which provide immunological memory by inserting short DNA sequences from phage and other parasitic DNA elements into CRISPR loci on the host genome 2 . Whereas CRISPR loci evolve rapidly in natural environments 3,4 , bacterial species typically evolve phage resistance by the mutation or loss of phage receptors under laboratory conditions 5,6 . Here we report how this discrepancy may in part be explained by differences in the biotic complexity of in vitro and natural environments 7,8 . Specifically, by using the opportunistic pathogen Pseudomonas aeruginosa and its phage DMS3vir, we show that coexistence with other human pathogens amplifies the fitness trade-offs associated with the mutation of phage receptors, and therefore tips the balance in favour of the evolution of CRISPR-based resistance. We also demonstrate that this has important knock-on effects for the virulence of P. aeruginosa, which became attenuated only if the bacteria evolved surface-based resistance. Our data reveal that the biotic complexity of microbial communities in natural environments is an important driver of the evolution of CRISPR-Cas adaptive immunity, with key implications for bacterial fitness and virulence. |
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| معلومات مُعتمدة: | 714478 International ERC_ European Research Council |
| المشرفين على المادة: | 0 (Receptors, Virus) |
| تواريخ الأحداث: | Date Created: 20191025 Date Completed: 20191119 Latest Revision: 20231019 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC6837874 |
| DOI: | 10.1038/s41586-019-1662-9 |
| PMID: | 31645729 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1476-4687 |
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| DOI: | 10.1038/s41586-019-1662-9 |