دورية أكاديمية
Entomotoxic Activity of Prasiola crispa (Antarctic Algae) in Nauphoeta cinerea Cockroaches: Identification of Main Steroidal Compounds.
العنوان: | Entomotoxic Activity of Prasiola crispa (Antarctic Algae) in Nauphoeta cinerea Cockroaches: Identification of Main Steroidal Compounds. |
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المؤلفون: | Holken Lorensi G; Laboratório de Neurobiologia e Toxinologia (LANETOX),Universidade Federal do Pampa, Campus São Gabriel, São Gabriel, RS 97307-020, Brazil. grazielahl@gmail.com., Soares Oliveira R; Laboratório de Neurobiologia e Toxinologia (LANETOX),Universidade Federal do Pampa, Campus São Gabriel, São Gabriel, RS 97307-020, Brazil. raquelsoaresoliveira@yahoo.com.br., Leal AP; Programa de Pós-Graduação em Bioquímica Toxicológica, PPGBtox, Universidade Federal de Santa Maria, UFSM, Bairro Camobi, Santa Maria, RS 9705-900, Brazil. allan-leal@hotmail.com., Zanatta AP; Laboratório de Neurobiologia e Toxinologia (LANETOX),Universidade Federal do Pampa, Campus São Gabriel, São Gabriel, RS 97307-020, Brazil. anapaulabiotech@gmail.com., Moreira de Almeida CG; Instituto do Cérebro (INSCER), Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS 90610-000, Brazil. carlos.gabriel@acad.pucrs.br., Barreto YC; Laboratório de Neurobiologia e Toxinologia (LANETOX),Universidade Federal do Pampa, Campus São Gabriel, São Gabriel, RS 97307-020, Brazil. barreto78@outlook.com., Eduarda Rosa M; Laboratório de Neurobiologia e Toxinologia (LANETOX),Universidade Federal do Pampa, Campus São Gabriel, São Gabriel, RS 97307-020, Brazil. rosaedumaria@gmail.com., de Brum Vieira P; Laboratório de Neurobiologia e Toxinologia (LANETOX),Universidade Federal do Pampa, Campus São Gabriel, São Gabriel, RS 97307-020, Brazil. patriciasbrum@yahoo.com.br.; Grupo de Pesquisa em Estresse Oxidativo e Sinalização Celular, Universidade Federal do Pampa, Campus São Gabriel, São Gabriel, RS 97307-020, Brazil. patriciasbrum@yahoo.com.br., Brito Ramos CJ; Programa de Pós-Graduação em Biodiversidade Neotropical, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ 22290-255, Brazil. cjbramos@gmail.com., de Carvalho Victoria F; Núcleo de Estudos da Vegetação Antártica (NEVA), Universidade Federal do Pampa, Campus São Gabriel, São Gabriel, Rio Grande do Sul 97307-020, Brazil. filipevictoria@unipampa.edu.br., Batista Pereira A; Núcleo de Estudos da Vegetação Antártica (NEVA), Universidade Federal do Pampa, Campus São Gabriel, São Gabriel, Rio Grande do Sul 97307-020, Brazil. antoniopereira@unipampa.edu.br., LaneuvilleTeixeira V; Programa de Pós-Graduação em Ciências e Biotecnologia, Instituto de Biologia, Universidade Federal Fluminense, Centro, Niterói, RJ 24020-141, Brazil. valerialaneuville@gmail.com.; Programa de Pós-Graduação em Biodiversidade Neotropical, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ 22290-255, Brazil. valerialaneuville@gmail.com., Dal Belo CA; Laboratório de Neurobiologia e Toxinologia (LANETOX),Universidade Federal do Pampa, Campus São Gabriel, São Gabriel, RS 97307-020, Brazil. charistonbelo@unipampa.edu.br.; Programa de Pós-Graduação em Bioquímica Toxicológica, PPGBtox, Universidade Federal de Santa Maria, UFSM, Bairro Camobi, Santa Maria, RS 9705-900, Brazil. charistonbelo@unipampa.edu.br. |
المصدر: | Marine drugs [Mar Drugs] 2019 Oct 10; Vol. 17 (10). Date of Electronic Publication: 2019 Oct 10. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101213729 Publication Model: Electronic Cited Medium: Internet ISSN: 1660-3397 (Electronic) Linking ISSN: 16603397 NLM ISO Abbreviation: Mar Drugs Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2003]- |
مواضيع طبية MeSH: | Cockroaches*, Chlorophyta/*chemistry , Insecticides/*chemistry , Phytosterols/*analysis , Plant Extracts/*chemistry, Animals ; Antarctic Regions ; Hexanes/chemistry ; Insecticides/isolation & purification ; Lethal Dose 50 ; Plant Extracts/isolation & purification |
مستخلص: | Prasiola crispa is a macroscopic green algae found in abundance in Antarctica ice free areas. Prasiola crispan -hexaneextract (HPC) induced insecticidal activity in Nauphoeta cinerea cockroaches after 24 h of exposure. The chemical analysis of HPC revealed the presence of the followingphytosterols: β-sitosterol, campesterol and stigmasterol. The incubation of cockroach semi-isolated heart preparations with HPC caused a significant negative chronotropic activity in the heartbeats. HPC affected the insect neuromuscular function by inducing a complete inhibition of the cockroach leg-muscle twitch tension. When the isolated phytosterols were injected at in vivo cockroach neuromuscular preparations, there was a progressive inhibition of muscle twitches on the following order of potency: β-sitosterol > campesterol > stigmasterol. HPC also provoked significant behavioral alterations, characterized by the increase or decrease of cockroach grooming activity, depending on the dose assayed. Altogether, the results presented here corroborate the insecticide potential of Prasiola crispa Antarctic algae. They also revealed the presence of phytosterols and the involvement of these steroidal compounds in the entomotoxic activity of the algae, potentially by modulating octopaminergic-cholinergic pathways. Further phytochemical-combined bioguided analysis of the HPC will unveil novel bioactive compounds that might be an accessory to the insecticide activity of the algae. |
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معلومات مُعتمدة: | Finance Code 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; 063/2010 Toxinologia Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; 003/2011 Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul; 187/2018 Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul; 301964/2018 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 05/2019 Universidade Federal do Pampa |
فهرسة مساهمة: | Keywords: Antarctic algae; biological activity; insecticide; marine natural products; marine steroids |
المشرفين على المادة: | 0 (Hexanes) 0 (Insecticides) 0 (Phytosterols) 0 (Plant Extracts) |
تواريخ الأحداث: | Date Created: 20191030 Date Completed: 20200320 Latest Revision: 20200320 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC6835979 |
DOI: | 10.3390/md17100573 |
PMID: | 31658661 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1660-3397 |
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DOI: | 10.3390/md17100573 |