دورية أكاديمية
أعرض في EDS

Expediting the sampling, decalcification, and forensic DNA analysis of large elephant ivory seizures to aid investigations and prosecutions.

التفاصيل البيبلوغرافية
العنوان: Expediting the sampling, decalcification, and forensic DNA analysis of large elephant ivory seizures to aid investigations and prosecutions.
المؤلفون: Ewart KM; TRACE Wildlife Forensics Network, Edinburgh, Scotland, United Kingdom. Electronic address: kyle.ewart@tracenetwork.org., Lightson AL; TRACE Wildlife Forensics Network, Edinburgh, Scotland, United Kingdom., Sitam FT; National Wildlife Forensic Laboratory, Department of Wildlife and National Parks (PERHILITAN), Kuala Lumpur, Malaysia., Rovie-Ryan JJ; National Wildlife Forensic Laboratory, Department of Wildlife and National Parks (PERHILITAN), Kuala Lumpur, Malaysia., Mather N; Chalmers St, Redfern, Sydney, Australia., McEwing R; TRACE Wildlife Forensics Network, Edinburgh, Scotland, United Kingdom.
المصدر: Forensic science international. Genetics [Forensic Sci Int Genet] 2020 Jan; Vol. 44, pp. 102187. Date of Electronic Publication: 2019 Oct 14.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101317016 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-0326 (Electronic) Linking ISSN: 18724973 NLM ISO Abbreviation: Forensic Sci Int Genet Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier
مواضيع طبية MeSH: DNA Fingerprinting* , Decalcification Technique* , Efficiency* , Specimen Handling*, Elephants/*genetics , Forensic Genetics/*methods, Africa ; Animals ; Commerce/legislation & jurisprudence ; Conservation of Natural Resources/legislation & jurisprudence ; Crime/legislation & jurisprudence ; DNA, Mitochondrial/genetics ; Humans ; Malaysia ; Polymerase Chain Reaction
مستخلص: The illegal ivory trade continues to drive elephant poaching. Large ivory seizures in Africa and Asia are still commonplace. Wildlife forensics is recognised as a key enforcement tool to combat this trade. However, the time and resources required to effectively test large ivory seizures is often prohibitive. This limits or delays testing, which may impede investigations and/or prosecutions. Typically, DNA analysis of an ivory seizure involves pairing and sorting the tusks, sampling the tusks, powdering the sample, decalcification, then DNA extraction. Here, we optimize the most time-consuming components of this process: sampling and decalcification. Firstly, using simulations, we demonstrate that tusks do not need to be paired to ensure an adequate number of unique elephants are sampled in a large seizure. Secondly, we determined that directly powdering the ivory using a Dremel drill with a high-speed cutter bit, instead of cutting the ivory with a circular saw and subsequently powdering the sample in liquid nitrogen with a freezer mill, produces comparable results. Finally, we optimized a rapid 2 -h decalcification protocol that produces comparable results to a standard 3-day protocol. We tested/optimised the protocols on 33 raw and worked ivory samples, and demonstrated their utility on a case study, successfully identifying 94% of samples taken from 123 tusks. Using these new rapid protocols, the entire sampling and DNA extraction process takes less than one day and requires less-expensive equipment. We expect that the implementation of these rapid protocols will promote more consistent and timely testing of ivory seizures suitable for enforcement action.
(Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Decalcification; Ivory; Sampling; Wildlife forensics
المشرفين على المادة: 0 (DNA, Mitochondrial)
تواريخ الأحداث: Date Created: 20191101 Date Completed: 20210308 Latest Revision: 20210308
رمز التحديث: 20231215
DOI: 10.1016/j.fsigen.2019.102187
PMID: 31670244
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-0326
DOI:10.1016/j.fsigen.2019.102187