دورية أكاديمية
MCRS1 is essential for epiblast development during early mouse embryogenesis.
| العنوان: | MCRS1 is essential for epiblast development during early mouse embryogenesis. |
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| المؤلفون: | Cui W; Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts, USA.; Animal Models Core Facility, Institute for Applied Life Sciences (IALS), University of Massachusetts, Amherst, Massachusetts, USA., Cheong A; Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts, USA., Wang Y; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People's Republic of China., Tsuchida Y; Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts, USA., Liu Y; Anhui Province Key Laboratory of Embryo Development and Reproductive Regulation, Anhui Province Key Laboratory of Environmental Hormone and Reproduction, Fuyang Normal University, Fuyang, Anhui, People's Republic of China., Tremblay KD; Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts, USA., Mager J; Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts, USA. |
| المصدر: | Reproduction (Cambridge, England) [Reproduction] 2020 Jan; Vol. 159 (1), pp. 1-13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published for the Society for Reproduction and Fertility by BioScientifica Country of Publication: England NLM ID: 100966036 Publication Model: Print Cited Medium: Internet ISSN: 1741-7899 (Electronic) Linking ISSN: 14701626 NLM ISO Abbreviation: Reproduction Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2004->: Bristol, UK : Published for the Society for Reproduction and Fertility by BioScientifica Original Publication: Cambridge, UK : Journals of Reproduction and Fertility, Ltd. c2001- |
| مواضيع طبية MeSH: | Embryonic Development* , Gene Expression Regulation, Developmental* , Mutation*, Blastocyst Inner Cell Mass/*cytology , Embryo, Mammalian/*cytology , Germ Layers/*cytology , RNA-Binding Proteins/*physiology, Animals ; Blastocyst Inner Cell Mass/metabolism ; Cell Differentiation ; Cell Lineage ; Embryo, Mammalian/metabolism ; Embryonic Stem Cells/cytology ; Embryonic Stem Cells/metabolism ; Female ; Germ Layers/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout |
| مستخلص: | Microspherule protein 1 (MCRS1, also known as MSP58) is an evolutionarily conserved protein that has been implicated in various biological processes. Although a variety of functions have been attributed to MCRS1 in vitro, mammalian MCRS1 has not been studied in vivo. Here we report that MCRS1 is essential during early murine development. Mcrs1 mutant embryos exhibit normal morphology at the blastocyst stage but cannot be recovered at gastrulation, suggesting an implantation failure. Outgrowth (OG) assays reveal that mutant blastocysts do not form a typical inner cell mass (ICM) colony, the source of embryonic stem cells (ESCs). Surprisingly, cell death and histone H4 acetylation analysis reveal that apoptosis and global H4 acetylation are normal in mutant blastocysts. However, analysis of lineage specification reveals that while the trophoblast and primitive endoderm are properly specified, the epiblast lineage is compromised and exhibits a severe reduction in cell number. In summary, our study demonstrates the indispensable role of MCRS1 in epiblast development during early mammalian embryogenesis. |
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| معلومات مُعتمدة: | R01 HD083311 United States HD NICHD NIH HHS |
| المشرفين على المادة: | 0 (Mcrs1 protein, mouse) 0 (RNA-Binding Proteins) |
| تواريخ الأحداث: | Date Created: 20191101 Date Completed: 20201030 Latest Revision: 20210101 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC7431978 |
| DOI: | 10.1530/REP-19-0334 |
| PMID: | 31671403 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1741-7899 |
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| DOI: | 10.1530/REP-19-0334 |