دورية أكاديمية
أعرض في EDS

Genetic basis for the cooperative bioactivation of plant lignans by Eggerthella lenta and other human gut bacteria.

التفاصيل البيبلوغرافية
العنوان: Genetic basis for the cooperative bioactivation of plant lignans by Eggerthella lenta and other human gut bacteria.
المؤلفون: Bess EN; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.; Department of Chemistry, University of California, Irvine, Irvine, CA, USA.; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, USA., Bisanz JE; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA., Yarza F; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA., Bustion A; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA., Rich BE; Department of Chemistry, University of California, Irvine, Irvine, CA, USA., Li X; University of Hawaii Cancer Center, Honolulu, HI, USA., Kitamura S; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA., Waligurski E; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA., Ang QY; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA., Alba DL; Diabetes Center, University of California San Francisco, San Francisco, CA, USA., Spanogiannopoulos P; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA., Nayfach S; United States Department of Energy Joint Genome Institute, Walnut Creek, CA, USA., Koliwad SK; Diabetes Center, University of California San Francisco, San Francisco, CA, USA., Wolan DW; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA., Franke AA; University of Hawaii Cancer Center, Honolulu, HI, USA., Turnbaugh PJ; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA. peter.turnbaugh@ucsf.edu.; Chan Zuckerberg Biohub, San Francisco, CA, USA. peter.turnbaugh@ucsf.edu.
المصدر: Nature microbiology [Nat Microbiol] 2020 Jan; Vol. 5 (1), pp. 56-66. Date of Electronic Publication: 2019 Nov 04.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101674869 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2058-5276 (Electronic) Linking ISSN: 20585276 NLM ISO Abbreviation: Nat Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Publishing Group, [2016]-
مواضيع طبية MeSH: Gene Expression Profiling*, Actinobacteria/*genetics , Gastrointestinal Microbiome/*genetics , Lignans/*metabolism, Actinobacteria/classification ; Actinobacteria/metabolism ; Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Biotransformation ; Genome, Bacterial/genetics ; Humans ; Lignans/chemistry ; Metabolic Networks and Pathways/genetics ; Mice ; Microbial Consortia/genetics ; Phylogeny ; Species Specificity
مستخلص: Plant-derived lignans, consumed daily by most individuals, are thought to protect against cancer and other diseases 1 ; however, their bioactivity requires gut bacterial conversion to enterolignans 2 . Here, we dissect a four-species bacterial consortium sufficient for all five reactions in this pathway. A single enzyme (benzyl ether reductase, encoded by the gene ber) was sufficient for the first two biotransformations, variable between strains of Eggerthella lenta, critical for enterolignan production in gnotobiotic mice and unique to Coriobacteriia. Transcriptional profiling (RNA sequencing) independently identified ber and genomic loci upregulated by each of the remaining substrates. Despite their low abundance in gut microbiomes and restricted phylogenetic range, all of the identified genes were detectable in the distal gut microbiomes of most individuals living in northern California. Together, these results emphasize the importance of considering strain-level variations and bacterial co-occurrence to gain a mechanistic understanding of the bioactivation of plant secondary metabolites by the human gut microbiome.
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معلومات مُعتمدة: R21 CA227232 United States CA NCI NIH HHS; M01 RR001271 United States RR NCRR NIH HHS; P30 DK098722 United States DK NIDDK NIH HHS; R01 HL122593 United States HL NHLBI NIH HHS; P30 CA071789 United States CA NCI NIH HHS
المشرفين على المادة: 0 (Bacterial Proteins)
0 (Lignans)
SCR Organism: Eggerthella lenta
تواريخ الأحداث: Date Created: 20191106 Date Completed: 20200717 Latest Revision: 20220420
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6941677
DOI: 10.1038/s41564-019-0596-1
PMID: 31686027
قاعدة البيانات: MEDLINE
الوصف
تدمد:2058-5276
DOI:10.1038/s41564-019-0596-1