دورية أكاديمية
أعرض في EDS

Resistome analyses of sputum from COPD and healthy subjects reveals bacterial load-related prevalence of target genes.

التفاصيل البيبلوغرافية
العنوان: Resistome analyses of sputum from COPD and healthy subjects reveals bacterial load-related prevalence of target genes.
المؤلفون: Ramsheh MY; Department of Respiratory Sciences, University of Leicester, Leicester, UK., Haldar K; Department of Respiratory Sciences, University of Leicester, Leicester, UK., Bafadhel M; Respiratory Medicine Unit, Nuffield Department of Medicine, University of Oxford, Oxford, UK., George L; Department of Respiratory Sciences, University of Leicester, Leicester, UK., Free RC; Department of Respiratory Sciences, University of Leicester, Leicester, UK., John C; Department of Health Sciences, University of Leicester, Leicester, UK., Reeve NF; Department of Health Sciences, University of Leicester, Leicester, UK., Ziegler-Heitbrock L; Helmholtz Zentrum Muenchen and Asklepios-Klinik, EvA Study Center, Gauting, Germany., Gut I; Centre for Genomic Regulation, Barcelona Institute for Science and Technology, 5CNAG-CRG Centre Nacional d'Anàlisi Genòmica, Barcelona, Spain., Singh D; Medicines Evaluation Unit, University Hospital of South Manchester, University of Manchester, Manchester, UK., Mistry V; Department of Respiratory Sciences, University of Leicester, Leicester, UK., Tobin MD; Department of Health Sciences, University of Leicester, Leicester, UK., Oggioni MR; Genetics and Genome Biology, University of Leicester, Leicester, UK., Brightling C; Department of Respiratory Sciences, University of Leicester, Leicester, UK., Barer MR; Department of Respiratory Sciences, University of Leicester, Leicester, UK mrb19@leicester.ac.uk.
المصدر: Thorax [Thorax] 2020 Jan; Vol. 75 (1), pp. 8-16. Date of Electronic Publication: 2019 Nov 07.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: British Medical Assn Country of Publication: England NLM ID: 0417353 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1468-3296 (Electronic) Linking ISSN: 00406376 NLM ISO Abbreviation: Thorax Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : British Medical Assn.
مواضيع طبية MeSH: Drug Resistance, Bacterial/*genetics , Pulmonary Disease, Chronic Obstructive/*microbiology , Pulmonary Disease, Chronic Obstructive/*physiopathology , Sputum/*microbiology, Aged ; Bacterial Load ; Female ; Genes, Bacterial ; Humans ; Male ; Microbiota ; Middle Aged ; Polymerase Chain Reaction
مستخلص: Background: Antibiotic resistance is a major global threat. We hypothesised that the chronic obstructive pulmonary disease (COPD) airway is a reservoir of antimicrobial resistance genes (ARGs) that associate with microbiome-specific COPD subgroups.
Objective: To determine the resistance gene profiles in respiratory samples from COPD patients and healthy volunteers.
Methods: Quantitative PCR targeting 279 specific ARGs was used to profile the resistomes in sputum from subjects with COPD at stable, exacerbation and recovery visits (n=55; COPD-BEAT study), healthy controls with (n=7) or without (n=22) exposure to antibiotics in the preceding 12 months (EXCEED study) and in bronchial brush samples from COPD (n=8) and healthy controls (n=7) (EvA study).
Results: ARG mean (SEM) prevalence was greater in stable COPD samples (35.2 (1.6)) than in healthy controls (27.6 (1.7); p=0.004) and correlated with total bacterial abundance (r 2 =0.23; p<0.001). Prevalence of ARG positive signals in individuals was not related to COPD symptoms, lung function or their changes at exacerbation. In the COPD subgroups designated High γProteobacteria and High Firmicutes, ARG prevalence was not different at stable state but significantly declined from stable through exacerbation to recovery in the former (p=0.011) without changes in total bacterial abundance. The ARG patterns were similar in COPD versus health, COPD microbiome-subgroups and between sputum and bronchoscopic samples independent of antibiotic exposure in the last 12 months.
Conclusions: ARGs are highly prevalent in sputum, broadly in proportion to bacterial abundance in both healthy and COPD subjects. Thus, COPD appears to be an ARG reservoir due to high levels of bacterial colonisation.
Competing Interests: Competing interests: None declared.
(© Author(s) (or their employer(s)) 2020. No commercial re-use. See rights and permissions. Published by BMJ.)
معلومات مُعتمدة: G0601369 United Kingdom MRC_ Medical Research Council; G0801980 United Kingdom MRC_ Medical Research Council; MR/P00167X/1 United Kingdom MRC_ Medical Research Council
فهرسة مساهمة: Keywords: COPD exacerbations; bacterial infection; infection control
تواريخ الأحداث: Date Created: 20191109 Date Completed: 20200810 Latest Revision: 20210110
رمز التحديث: 20221213
DOI: 10.1136/thoraxjnl-2019-213485
PMID: 31699806
قاعدة البيانات: MEDLINE
الوصف
تدمد:1468-3296
DOI:10.1136/thoraxjnl-2019-213485