دورية أكاديمية
Distinct IL-1α-responsive enhancers promote acute and coordinated changes in chromatin topology in a hierarchical manner.
العنوان: | Distinct IL-1α-responsive enhancers promote acute and coordinated changes in chromatin topology in a hierarchical manner. |
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المؤلفون: | Weiterer SS; Rudolf Buchheim Institute of Pharmacology, Justus Liebig University Giessen, Giessen, Germany., Meier-Soelch J; Rudolf Buchheim Institute of Pharmacology, Justus Liebig University Giessen, Giessen, Germany., Georgomanolis T; Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany., Mizi A; Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany.; Department of Pathology, University Medical Center Göttingen, Göttingen, Germany., Beyerlein A; Rudolf Buchheim Institute of Pharmacology, Justus Liebig University Giessen, Giessen, Germany., Weiser H; Rudolf Buchheim Institute of Pharmacology, Justus Liebig University Giessen, Giessen, Germany., Brant L; Department of Pathology, University Medical Center Göttingen, Göttingen, Germany., Mayr-Buro C; Rudolf Buchheim Institute of Pharmacology, Justus Liebig University Giessen, Giessen, Germany., Jurida L; Rudolf Buchheim Institute of Pharmacology, Justus Liebig University Giessen, Giessen, Germany., Beuerlein K; Rudolf Buchheim Institute of Pharmacology, Justus Liebig University Giessen, Giessen, Germany., Müller H; Rudolf Buchheim Institute of Pharmacology, Justus Liebig University Giessen, Giessen, Germany., Weber A; Rudolf Buchheim Institute of Pharmacology, Justus Liebig University Giessen, Giessen, Germany., Tenekeci U; Rudolf Buchheim Institute of Pharmacology, Justus Liebig University Giessen, Giessen, Germany., Dittrich-Breiholz O; Research Core Unit Genomics, Institute of Physiological Chemistry, Medical School Hannover, Hannover, Germany., Bartkuhn M; Institute for Genetics, Justus Liebig University Giessen, Giessen, Germany., Nist A; Genomics Core Facility and Institute of Molecular Oncology, Philipps University Marburg, Marburg, Germany., Stiewe T; Genomics Core Facility and Institute of Molecular Oncology, Philipps University Marburg, Marburg, Germany.; Member of the German Center for Lung Research (DZL), Giessen, Germany., van IJcken WF; Center for Biomics, Erasmus Medical Center, Rotterdam, The Netherlands., Riedlinger T; Institute of Biochemistry, Justus Liebig University Giessen, Giessen, Germany., Schmitz ML; Member of the German Center for Lung Research (DZL), Giessen, Germany.; Institute of Biochemistry, Justus Liebig University Giessen, Giessen, Germany., Papantonis A; Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany.; Department of Pathology, University Medical Center Göttingen, Göttingen, Germany., Kracht M; Rudolf Buchheim Institute of Pharmacology, Justus Liebig University Giessen, Giessen, Germany.; Member of the German Center for Lung Research (DZL), Giessen, Germany. |
المصدر: | The EMBO journal [EMBO J] 2020 Jan 02; Vol. 39 (1), pp. e101533. Date of Electronic Publication: 2019 Nov 07. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 8208664 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2075 (Electronic) Linking ISSN: 02614189 NLM ISO Abbreviation: EMBO J Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2024- : [London] : Nature Publishing Group Original Publication: Eynsham, Oxford, England : Published for the European Molecular Biology Organization by IRL Press, [c1982- |
مواضيع طبية MeSH: | Chromatin/*metabolism , Enhancer Elements, Genetic/*genetics , Gene Expression Regulation/*drug effects , Inflammation Mediators/*metabolism , Interleukin-1alpha/*pharmacology , MAP Kinase Kinase Kinases/*metabolism , NF-kappa B/*metabolism, Binding Sites ; Cells, Cultured ; Chemokines/metabolism ; Chromatin/chemistry ; Chromatin/genetics ; HeLa Cells ; Humans ; MAP Kinase Kinase Kinases/genetics ; NF-kappa B/genetics ; Signal Transduction ; Tumor Necrosis Factor-alpha/pharmacology |
مستخلص: | How cytokine-driven changes in chromatin topology are converted into gene regulatory circuits during inflammation still remains unclear. Here, we show that interleukin (IL)-1α induces acute and widespread changes in chromatin accessibility via the TAK1 kinase and NF-κB at regions that are highly enriched for inflammatory disease-relevant SNPs. Two enhancers in the extended chemokine locus on human chromosome 4 regulate the IL-1α-inducible IL8 and CXCL1-3 genes. Both enhancers engage in dynamic spatial interactions with gene promoters in an IL-1α/TAK1-inducible manner. Microdeletions of p65-binding sites in either of the two enhancers impair NF-κB recruitment, suppress activation and biallelic transcription of the IL8/CXCL2 genes, and reshuffle higher-order chromatin interactions as judged by i4C interactome profiles. Notably, these findings support a dominant role of the IL8 "master" enhancer in the regulation of sustained IL-1α signaling, as well as for IL-8 and IL-6 secretion. CRISPR-guided transactivation of the IL8 locus or cross-TAD regulation by TNFα-responsive enhancers in a different model locus supports the existence of complex enhancer hierarchies in response to cytokine stimulation that prime and orchestrate proinflammatory chromatin responses downstream of NF-κB. (© 2019 The Authors. Published under the terms of the CC BY 4.0 license.) |
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معلومات مُعتمدة: | 162103480 International Deutsche Forschungsgemeinschaſt (DFG); 284237345 International Deutsche Forschungsgemeinschaſt (DFG); 183582903 International Deutsche Forschungsgemeinschaſt (DFG); 268555672 International Deutsche Forschungsgemeinschaſt (DFG); 197785619 International Deutsche Forschungsgemeinschaſt (DFG); 162103480 International Deutsche Forschungsgemeinschaſt (DFG); 183582903 International Deutsche Forschungsgemeinschaſt (DFG); 197785619 International Deutsche Forschungsgemeinschaſt (DFG); 285697699 International Deutsche Forschungsgemeinschaſt (DFG); 290613333 International Deutsche Forschungsgemeinschaſt (DFG); 24676099 International Deutsche Forschungsgemeinschaſt (DFG); 390649896 International Deutsche Forschungsgemeinschaſt (DFG); 111447 International Deutsche Krebshilfe; International Max-Planck Society; 24676099 International Excellence Cluster Cardio-Pulmonary System and Cardio-Pulmonary Institute (EXC 147: Kardiopulmonales System); 390649896 International Cardio-Pulmonary Institute (CPI); International DZL/UGMLC Program; International Center for Molecular Medicine Cologne (ZMMK) |
فهرسة مساهمة: | Keywords: IL-8; NF-κB; chromatin topology; interleukin-1; tumor necrosis factor-α |
سلسلة جزيئية: | GEO GSE64224; GSE52470; GSE134436 SRA PRJNA552438 |
المشرفين على المادة: | 0 (Chemokines) 0 (Chromatin) 0 (IL1A protein, human) 0 (Inflammation Mediators) 0 (Interleukin-1alpha) 0 (NF-kappa B) 0 (Tumor Necrosis Factor-alpha) EC 2.7.11.25 (MAP Kinase Kinase Kinases) EC 2.7.11.25 (MAP kinase kinase kinase 7) |
تواريخ الأحداث: | Date Created: 20191109 Date Completed: 20200713 Latest Revision: 20240327 |
رمز التحديث: | 20240327 |
مُعرف محوري في PubMed: | PMC6939198 |
DOI: | 10.15252/embj.2019101533 |
PMID: | 31701553 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1460-2075 |
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DOI: | 10.15252/embj.2019101533 |