دورية أكاديمية
Seed-borne endophytic Bacillus velezensis LHSB1 mediate the biocontrol of peanut stem rot caused by Sclerotium rolfsii.
| العنوان: | Seed-borne endophytic Bacillus velezensis LHSB1 mediate the biocontrol of peanut stem rot caused by Sclerotium rolfsii. |
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| المؤلفون: | Chen L; Collaborative Innovation Center of Henan Grain Crops, Henan Collaborative Innovation Center of Grain Storage and Security, Henan University of Technology, Zhengzhou, China., Wu YD; Collaborative Innovation Center of Henan Grain Crops, Henan Collaborative Innovation Center of Grain Storage and Security, Henan University of Technology, Zhengzhou, China., Chong XY; Collaborative Innovation Center of Henan Grain Crops, Henan Collaborative Innovation Center of Grain Storage and Security, Henan University of Technology, Zhengzhou, China., Xin QH; Collaborative Innovation Center of Henan Grain Crops, Henan Collaborative Innovation Center of Grain Storage and Security, Henan University of Technology, Zhengzhou, China., Wang DX; Collaborative Innovation Center of Henan Grain Crops, Henan Collaborative Innovation Center of Grain Storage and Security, Henan University of Technology, Zhengzhou, China., Bian K; Collaborative Innovation Center of Henan Grain Crops, Henan Collaborative Innovation Center of Grain Storage and Security, Henan University of Technology, Zhengzhou, China. |
| المصدر: | Journal of applied microbiology [J Appl Microbiol] 2020 Mar; Vol. 128 (3), pp. 803-813. Date of Electronic Publication: 2019 Nov 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2022- : Oxford : Oxford University Press Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997- |
| مواضيع طبية MeSH: | Biological Control Agents*, Arachis/*microbiology , Bacillus/*physiology , Basidiomycota/*growth & development , Plant Diseases/*prevention & control, Antifungal Agents/metabolism ; Antifungal Agents/pharmacology ; Bacillus/metabolism ; Basidiomycota/drug effects ; Endophytes/physiology ; Germination ; Hyphae/growth & development ; Plant Diseases/microbiology ; Seeds/microbiology |
| مستخلص: | Aims: This study aimed to obtain an antagonistic endophyte against Sclerotium rolfsii from peanut seeds, evaluate the biocontrol efficacy towards peanut stem rot and explore its antifungal mechanism against S. rolfsii. Methods and Results: Thirty-seven endophytic bacteria were isolated from peanut seeds, six of which exhibited stronger antagonistic activities against S. rolfsii (inhibition rate, IR of hyphae growth ≥70%). Strain LHSB1, the strongest antagonistic strain, was identified as Bacillus velezensis. LHSB1 showed 93·8% of radial growth inhibition of S. rolfsii hyphae and exhibited obvious antagonistic activity against another six pathogenic fungi of peanut. Pot experiments showed two different LHSB1 treatments both significantly reduced the disease incidence and severity of stem rot (P < 0·05) compared to the controls, and the biocontrol efficacy reached 62·6-70·8%, significantly higher than that of Carbendazim control (P < 0·05). Further analyses revealed LHSB1 culture filtrate significantly inhibited sclerotia formation and germination, caused the abnormalities and membrane integrity damage of S. rolfsii hyphae, which might be the possible mode of action of LHSB1 against S. rolfsii. Three antifungal lipopeptides bacillomycin A, surfactin A and fengycin A, were detected in LHSB1 culture extracts by UPLC-ESI-MS, which could be responsible for the biocontrol activity of LHSB1 against S. rolfsii. Conclusion: Our results suggested that the seed-borne endophytic B. velezensis LHSB1 would be a tremendous potential agent for the biocontrol of peanut stem rot caused by S. rolfsii. Significance and Impact of the Study: This comprehensive study provides a candidate endophytic biocontrol strain and reveals its antifungal mechanism against S. rolfsi. To the best of our knowledge, this is the first time that seed-borne endophytic B. velezensis was used as the biocontrol agent to control peanut stem rot. (© 2019 The Society for Applied Microbiology.) |
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| معلومات مُعتمدة: | 31401543 National Natural Science Foundation Project of China; 2017YFC1600800 National Key R&D Program of China; LQ2016101 National Top Youth Talent Support Program for Grain Industry; 182300410042 Natural Science Foundation Project of Henan Province; CARS-13 National Modern Agricultural Industry Technology System Construction Program |
| فهرسة مساهمة: | Keywords: Bacillus velezensis; Sclerotium rolfsii; biocontrol; peanut; sclerotia; seed endophyte; stem rot |
| سلسلة جزيئية: | GENBANK MN044879; MH394318; MH788975 |
| المشرفين على المادة: | 0 (Antifungal Agents) 0 (Biological Control Agents) |
| SCR Organism: | Bacillus velezensis |
| تواريخ الأحداث: | Date Created: 20191110 Date Completed: 20200424 Latest Revision: 20200424 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1111/jam.14508 |
| PMID: | 31705716 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1365-2672 |
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| DOI: | 10.1111/jam.14508 |