دورية أكاديمية
أعرض في EDS

Exoproteome profiling of Trypanosoma cruzi during amastigogenesis early stages.

التفاصيل البيبلوغرافية
العنوان: Exoproteome profiling of Trypanosoma cruzi during amastigogenesis early stages.
المؤلفون: Mandacaru SC; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., Queiroz RML; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil.; Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark., Alborghetti MR; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., de Oliveira LS; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., de Lima CMR; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., Bastos IMD; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., Santana JM; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., Roepstorff P; Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark., Ricart CAO; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil., Charneau S; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil.
المصدر: PloS one [PLoS One] 2019 Nov 22; Vol. 14 (11), pp. e0225386. Date of Electronic Publication: 2019 Nov 22 (Print Publication: 2019).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Chagas Disease/*parasitology , Proteomics/*methods , Protozoan Proteins/*metabolism , Trypanosoma cruzi/*pathogenicity, Chagas Disease/metabolism ; Chromatography, Liquid ; HeLa Cells ; Host-Parasite Interactions ; Humans ; Hydrogen-Ion Concentration ; Life Cycle Stages ; Tandem Mass Spectrometry ; Trypanosoma cruzi/metabolism
مستخلص: Chagas disease is caused by the protozoan Trypanosoma cruzi, affecting around 8 million people worldwide. After host cell invasion, the infective trypomastigote form remains 2-4 hours inside acidic phagolysosomes to differentiate into replicative amastigote form. In vitro acidic-pH-induced axenic amastigogenesis was used here to study this step of the parasite life cycle. After three hours of trypomastigote incubation in amastigogenesis promoting acidic medium (pH 5.0) or control physiological pH (7.4) medium samples were subjected to three rounds of centrifugation followed by ultrafiltration of the supernatants. The resulting exoproteome samples were trypsin digested and analysed by nano flow liquid chromatography coupled to tandem mass spectrometry. Computational protein identification searches yielded 271 and 483 protein groups in the exoproteome at pH 7.4 and pH 5.0, respectively, with 180 common proteins between both conditions. The total amount and diversity of proteins released by parasites almost doubled upon acidic incubation compared to control. Overall, 76.5% of proteins were predicted to be secreted by classical or non-classical pathways and 35.1% of these proteins have predicted transmembrane domains. Classical secretory pathway analysis showed an increased number of mucins and mucin-associated surface proteins after acidic incubation. However, the number of released trans-sialidases and surface GP63 peptidases was higher at pH 7.4. Trans-sialidases and mucins are anchored to the membrane and exhibit an enzyme-substrate relationship. In general, mucins are glycoproteins with immunomodulatory functions in Chagas disease, present mainly in the epimastigote and trypomastigote surfaces and could be enzymatically cleaved and released in the phagolysosome during amastigogenesis. Moreover, evidence for flagella discard during amastigogenesis are addressed. This study provides the first comparative analysis of the exoproteome during amastigogenesis, and the presented data evidence the dynamism of its profile in response to acidic pH-induced differentiation.
Competing Interests: The authors have declared that no competing interests exist.
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المشرفين على المادة: 0 (Protozoan Proteins)
تواريخ الأحداث: Date Created: 20191123 Date Completed: 20200320 Latest Revision: 20200320
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6874342
DOI: 10.1371/journal.pone.0225386
PMID: 31756194
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0225386