دورية أكاديمية
Recovery and cryopreservation of epididymal sperm from domestic cat using powdered coconut water (ACP-117c) and TRIS extenders.
| العنوان: | Recovery and cryopreservation of epididymal sperm from domestic cat using powdered coconut water (ACP-117c) and TRIS extenders. |
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| المؤلفون: | Barbosa BS; School of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Izzo RG; School of Veterinary Medicine, Federal Rural University of Semi-Arid, Mossoró, RN, Brazil., Silva HVR; School of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Nunes TGP; School of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Brito BF; School of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Silva TFPD; School of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Silva LDMD; School of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil. Electronic address: lucia.daniel.machado@hotmail.com. |
| المصدر: | Cryobiology [Cryobiology] 2020 Feb 01; Vol. 92, pp. 103-108. Date of Electronic Publication: 2019 Nov 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0006252 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2392 (Electronic) Linking ISSN: 00112240 NLM ISO Abbreviation: Cryobiology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : Amsterdam : Elsevier Original Publication: San Diego, CA : Academic Press |
| مواضيع طبية MeSH: | Cryopreservation/*methods , Cryoprotective Agents/*pharmacology , Semen Analysis/*veterinary , Semen Preservation/*methods , Spermatozoa/*drug effects, Animals ; Cats ; Cocos ; Epididymis/cytology ; Freezing ; Glycerol/pharmacology ; Male ; Powders/pharmacology ; Sperm Motility ; Spermatozoa/cytology |
| مستخلص: | Cryopreservation of cats epididymal spermatozoa allows the conservation of the genetic material and the study of the cryogenic effect applied to the gametes of other felines. However, this biotechnique still presents variable results, being necessary the investigation of alternative extenders. Powdered coconut water (ACP-117c) has been efficient in the sperm freezing of several species and in the cat sperm refrigeration. Therefore, we aimed to evaluate the effect of the freezing stages and the quality of the cats' epididymal spermatozoa after thawing, using ACP-117c. Epididymides (n = 36) from 18 cats were processed using TRIS (n = 18) or ACP-117c (n = 18) for sperm recovery. The sperm were immediately evaluated. Then, this was cooled, glycerolized, frozen and thawed, and re-evaluated at each stage for sperm kinetics by Computer Assisted Semen Analysis, viability, functionality (HOST), mitochondrial activity (DAB) and morphology. There was a reduction in total motility and progressive motility after thawing in both groups, and TRIS was superior to ACP-117c. The curvilinear velocity reduced after thawing with ACP-117c. Viability decreased after glycerolization in TRIS. Although it also reduced after thawing in both groups, it was higher in TRIS. There was no change on HOST. Mitochondrial activity decreased during the cryopreservation steps for both extenders. Nevertheless, TRIS presented a higher percentage of spermatozoa from DAB class I and II after thawing. Morphology did not differ between extenders. Therefore, ACP-117c is an alternative for the recovery of cat epididymal spermatozoa; however, it is not efficient for freezing. Glycerolization and thawing are the most critical stages, regardless of the extender. Competing Interests: Declaration of competing interest The authors have stated that there are no competing interests. None of the authors has financial or personal relationships that may influence or distort the content of the article. (Copyright © 2019 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Alternative extender; Conservation; Felines; Spermatozoa |
| المشرفين على المادة: | 0 (Cryoprotective Agents) 0 (Powders) PDC6A3C0OX (Glycerol) |
| تواريخ الأحداث: | Date Created: 20191127 Date Completed: 20200915 Latest Revision: 20200915 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.cryobiol.2019.11.042 |
| PMID: | 31770529 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2392 |
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| DOI: | 10.1016/j.cryobiol.2019.11.042 |