دورية أكاديمية
Metatranscriptomic reconstruction reveals RNA viruses with the potential to shape carbon cycling in soil.
| العنوان: | Metatranscriptomic reconstruction reveals RNA viruses with the potential to shape carbon cycling in soil. |
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| المؤلفون: | Starr EP; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720., Nuccio EE; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550., Pett-Ridge J; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550., Banfield JF; Department of Earth and Planetary Science, University of California, Berkeley, CA 94720; jbanfield@berkeley.edu mkfstone@berkeley.edu.; Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720.; Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720.; Chan Zuckerberg Biohub, San Francisco, CA 94158.; Innovative Genomics Institute, Berkeley, CA 94720., Firestone MK; Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720; jbanfield@berkeley.edu mkfstone@berkeley.edu.; Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720. |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2019 Dec 17; Vol. 116 (51), pp. 25900-25908. Date of Electronic Publication: 2019 Nov 26. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Soil Microbiology* , Transcriptome*, Carbon Cycle/*physiology , RNA Viruses/*genetics , RNA Viruses/*metabolism, Bacteria/virology ; Bacteriophages/classification ; Bacteriophages/genetics ; Bacteriophages/metabolism ; Carbon/metabolism ; Ecology ; Fungi/virology ; Host Microbial Interactions ; Leviviridae ; Phylogeny ; RNA Viruses/classification ; Rhizosphere ; Sequence Analysis ; Soil |
| مستخلص: | Viruses impact nearly all organisms on Earth, with ripples of influence in agriculture, health, and biogeochemical processes. However, very little is known about RNA viruses in an environmental context, and even less is known about their diversity and ecology in soil, 1 of the most complex microbial systems. Here, we assembled 48 individual metatranscriptomes from 4 habitats within a planted soil sampled over a 22-d time series: Rhizosphere alone, detritosphere alone, rhizosphere with added root detritus, and unamended soil (4 time points and 3 biological replicates). We resolved the RNA viral community, uncovering a high diversity of viral sequences. We also investigated possible host organisms by analyzing metatranscriptome marker genes. Based on viral phylogeny, much of the diversity was Narnaviridae that may parasitize fungi or Leviviridae , which may infect Proteobacteria. Both host and viral communities appear to be highly dynamic, and rapidly diverged depending on experimental conditions. The viral and host communities were structured based on the presence of root litter. Clear temporal dynamics by Leviviridae and their hosts indicated that viruses were replicating. With this time-resolved analysis, we show that RNA viruses are diverse, abundant, and active in soil. When viral infection causes host cell death, it may mobilize cell carbon in a process that may represent an overlooked component of soil carbon cycling. Competing Interests: The authors declare no competing interest. (Copyright © 2019 the Author(s). Published by PNAS.) |
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| فهرسة مساهمة: | Keywords: metatranscriptome; phage; rhizosphere; soil; virus |
| سلسلة جزيئية: | GENBANK MN032676; MN036333; MK945893; MK946421 |
| المشرفين على المادة: | 0 (Soil) 7440-44-0 (Carbon) |
| تواريخ الأحداث: | Date Created: 20191128 Date Completed: 20200401 Latest Revision: 20231027 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC6926006 |
| DOI: | 10.1073/pnas.1908291116 |
| PMID: | 31772013 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1091-6490 |
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| DOI: | 10.1073/pnas.1908291116 |