دورية أكاديمية
Tetra-cationic platinum(II) porphyrins like a candidate photosensitizers to bind, selective and drug delivery for metastatic melanoma.
| العنوان: | Tetra-cationic platinum(II) porphyrins like a candidate photosensitizers to bind, selective and drug delivery for metastatic melanoma. |
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| المؤلفون: | Couto GK; Molecular and Cellular Oncology Research Group, Cancer Biotechnology Laboratory, Technological Development Center, Federal University of Pelotas, Pelotas, Brazil., Pacheco BS; Molecular and Cellular Oncology Research Group, Cancer Biotechnology Laboratory, Technological Development Center, Federal University of Pelotas, Pelotas, Brazil., Borba VM; Molecular and Cellular Oncology Research Group, Cancer Biotechnology Laboratory, Technological Development Center, Federal University of Pelotas, Pelotas, Brazil., Junior JCR; Molecular and Cellular Oncology Research Group, Cancer Biotechnology Laboratory, Technological Development Center, Federal University of Pelotas, Pelotas, Brazil., Oliveira TL; Molecular and Cellular Oncology Research Group, Cancer Biotechnology Laboratory, Technological Development Center, Federal University of Pelotas, Pelotas, Brazil., Segatto NV; Molecular and Cellular Oncology Research Group, Cancer Biotechnology Laboratory, Technological Development Center, Federal University of Pelotas, Pelotas, Brazil., Seixas FK; Molecular and Cellular Oncology Research Group, Cancer Biotechnology Laboratory, Technological Development Center, Federal University of Pelotas, Pelotas, Brazil., Acunha TV; Laboratory of Bioinorganic and Porphyrinoid Materials, Chemistry Department, Federal University of Santa Maria, Santa Maria, Brazil., Iglesias BA; Laboratory of Bioinorganic and Porphyrinoid Materials, Chemistry Department, Federal University of Santa Maria, Santa Maria, Brazil. Electronic address: bernardopgq@gmail.com., Collares T; Molecular and Cellular Oncology Research Group, Cancer Biotechnology Laboratory, Technological Development Center, Federal University of Pelotas, Pelotas, Brazil. Electronic address: collares.t@gmail.com. |
| المصدر: | Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2020 Jan; Vol. 202, pp. 111725. Date of Electronic Publication: 2019 Nov 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Switzerland NLM ID: 8804966 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2682 (Electronic) Linking ISSN: 10111344 NLM ISO Abbreviation: J Photochem Photobiol B Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Elsevier Sequoia, 1987- |
| مواضيع طبية MeSH: | Drug Carriers/*chemistry , Photosensitizing Agents/*chemistry , Platinum/*chemistry , Porphyrins/*chemistry, Animals ; Apoptosis/drug effects ; Binding Sites ; CHO Cells ; Caspase 3/genetics ; Caspase 3/metabolism ; Cations/chemistry ; Cell Cycle Checkpoints/drug effects ; Cell Line, Tumor ; Cricetinae ; Cricetulus ; Gene Expression Regulation/drug effects ; Humans ; Light ; Melanoma/metabolism ; Melanoma/pathology ; Molecular Docking Simulation ; Photosensitizing Agents/pharmacology ; Proto-Oncogene Proteins c-bcl-2/genetics ; Proto-Oncogene Proteins c-bcl-2/metabolism |
| مستخلص: | Photodynamic therapy (PDT) is an expanding treatment modality due to its minimally invasive localized activity and few adverse effects. This therapy requires photosensitive compounds, which have high sensitivity to light exposure. Thus, in this work, the in vitro antitumor activity of meso-tetra(3- and 4-pyridyl)porphyrins (3-TPyP and 4-TPyP) in metastatic melanoma cell (WM1366 line) and non-tumoral Ovarian lineage Chinese Hamister (CHO) was evaluated using photodynamic process. Cell viability tests, molecular docking, annexin V, confocal microscopy and qRT-PCR were performed. Our results show that both porphyrins inhibited the viability of metastatic melanoma cells when exposed to light and did not alter viability in the dark. In addition, they did not demonstrate cytotoxicity in non-tumor cells. Molecular coupling demonstrated platinum porphyrin affinity for the N-terminal region of APO B-100, LDL receptor, and therefore of the cells under study. Genes such as Caspase 3 and 9, P21, Bax / BCL2, MnSod and GSH showed increased expression. For meta isomer 3-PtTPyP treatment, caspase-9 and caspase-3 expression levels showed a 4.89 and 3.23-fold increase, respectively, while for the para isomer 4-PtTPyP, this change was 3.77 and 12.16-fold, respectively. We also observed an upregulated expression of p21, a protein well-known by its action in cell cycle arrest in a p53-dependent manner. Conclusion: 3-PtTPyP and 4-PtTPyP demonstrated antitumor effect on WM1366 cells, inducing apoptosis and significant alteration of cell cytoskeleton actin. Our work shows that platinum(II) porphyrins may be promising photosensitizers for the treatment of metastatic melanoma by PDT. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: APO B-100, Cancer; LDL receptor; Molecular docking; Photosensitizer; Platinum(II) porphyrins |
| المشرفين على المادة: | 0 (Cations) 0 (Drug Carriers) 0 (Photosensitizing Agents) 0 (Porphyrins) 0 (Proto-Oncogene Proteins c-bcl-2) 49DFR088MY (Platinum) EC 3.4.22.- (Caspase 3) |
| تواريخ الأحداث: | Date Created: 20191203 Date Completed: 20200128 Latest Revision: 20200128 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jphotobiol.2019.111725 |
| PMID: | 31790880 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2682 |
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| DOI: | 10.1016/j.jphotobiol.2019.111725 |