دورية أكاديمية

Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies.

التفاصيل البيبلوغرافية
العنوان: Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies.
المؤلفون: Fan CY; Department of Chemistry , National Tsing Hua University , Hsinchu , Taiwan . Email: cclin66@mx.nthu.edu.tw., Hou YR; Department of Chemistry , National Tsing Hua University , Hsinchu , Taiwan . Email: cclin66@mx.nthu.edu.tw., Adak AK; Department of Chemistry , National Tsing Hua University , Hsinchu , Taiwan . Email: cclin66@mx.nthu.edu.tw., Waniwan JT; Institute of Chemistry , Academia Sinica , Taipei , Taiwan . Email: yjchen@gate.sinica.edu.tw., Dela Rosa MAC; Institute of Chemistry , Academia Sinica , Taipei , Taiwan . Email: yjchen@gate.sinica.edu.tw., Low PY; Institute of Biological Chemistry , Academia Sinica , Taipei , Taiwan., Angata T; Institute of Biological Chemistry , Academia Sinica , Taipei , Taiwan., Hwang KC; Department of Chemistry , National Tsing Hua University , Hsinchu , Taiwan . Email: cclin66@mx.nthu.edu.tw., Chen YJ; Institute of Chemistry , Academia Sinica , Taipei , Taiwan . Email: yjchen@gate.sinica.edu.tw., Lin CC; Department of Chemistry , National Tsing Hua University , Hsinchu , Taiwan . Email: cclin66@mx.nthu.edu.tw.; Frontier Research Center on Fundamental and Applied Sciences of Matters , Hsinchu , Taiwan.; Department of Medicinal and Applied Chemistry , Kaohsiung Medical University , Kaohsiung , Taiwan.
المصدر: Chemical science [Chem Sci] 2019 Aug 05; Vol. 10 (37), pp. 8600-8609. Date of Electronic Publication: 2019 Aug 05 (Print Publication: 2019).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101545951 Publication Model: eCollection Cited Medium: Print ISSN: 2041-6520 (Print) Linking ISSN: 20416520 NLM ISO Abbreviation: Chem Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, UK : Royal Society of Chemistry, [2010]-
مستخلص: The utilization of immuno-magnetic nanoparticles (MNPs) for the selective capture, enrichment, and separation of specific glycoproteins from complicated biological samples is appealing for the discovery of disease biomarkers. Herein, MNPs were designed and anchored with abundant boronic acid (BA) and photoreactive alkyl diazirine (Diaz) functional groups to obtain permanently tethered Fc-fused Siglec-2 and antiserum amyloid A (SAA) mAb with the assistance of reversible boronate affinity and UV light activation in an orientation-controlled manner. The Siglec-2-Fc-functionalized MNPs showed excellent stability in fetal bovine serum (FBS) and excellent efficiency in the extraction of cell membrane glycoproteins. The anti-SAA mAb-functionalized MNPs maintained active Ab orientation and preserved antigen recognition capability in biological samples. Thus, the BA-Diaz-based strategy holds promise for the immobilization of glycoproteins, such as antibodies, with the original protein binding activity maintained, which can provide better enrichment for the sensitive detection of target proteins.
(This journal is © The Royal Society of Chemistry 2019.)
التعليقات: Erratum in: Chem Sci. 2022 Mar 10;13(13):3902. doi: 10.1039/d2sc90049a. (PMID: 35432915)
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تواريخ الأحداث: Date Created: 20191206 Latest Revision: 20240721
رمز التحديث: 20240721
مُعرف محوري في PubMed: PMC6844280
DOI: 10.1039/c9sc01613a
PMID: 31803435
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-6520
DOI:10.1039/c9sc01613a