دورية أكاديمية
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Crystal structure of the translation recovery factor Trf from Sulfolobus solfataricus.

التفاصيل البيبلوغرافية
العنوان: Crystal structure of the translation recovery factor Trf from Sulfolobus solfataricus.
المؤلفون: Kaiser M; Institute of Molecular Biosciences and Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Germany., Wurm JP; Institute of Molecular Biosciences and Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Germany., Märtens B; Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, Center of Molecular Biology, University of Vienna, Austria., Bläsi U; Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, Center of Molecular Biology, University of Vienna, Austria., Pogoryelov D; Institute of Biochemistry, Goethe-University Frankfurt, Germany., Wöhnert J; Institute of Molecular Biosciences and Center for Biomolecular Magnetic Resonance (BMRZ), Goethe-University Frankfurt, Germany.
المصدر: FEBS open bio [FEBS Open Bio] 2020 Feb; Vol. 10 (2), pp. 221-228. Date of Electronic Publication: 2019 Dec 18.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 101580716 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2211-5463 (Electronic) Linking ISSN: 22115463 NLM ISO Abbreviation: FEBS Open Bio Subsets: MEDLINE
أسماء مطبوعة: Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd.
Original Publication: Amsterdam : Elsevier, 2011-
مواضيع طبية MeSH: Archaeal Proteins/*ultrastructure , Peptide Initiation Factors/*ultrastructure , Prokaryotic Initiation Factors/*ultrastructure , Sulfolobus solfataricus/*metabolism, Archaeal Proteins/chemistry ; Archaeal Proteins/metabolism ; Binding Sites ; Carrier Proteins/metabolism ; Crystallography, X-Ray/methods ; Peptide Initiation Factors/chemistry ; Peptide Initiation Factors/metabolism ; Prokaryotic Initiation Factors/metabolism ; Protein Binding ; Sulfolobus solfataricus/genetics
مستخلص: During translation initiation, the heterotrimeric archaeal translation initiation factor 2 (aIF2) recruits the initiator tRNA i to the small ribosomal subunit. In the stationary growth phase and/or during nutrient stress, Sulfolobus solfataricus aIF2 has a second function: It protects leaderless mRNAs against degradation by binding to their 5'-ends. The S. solfataricus protein Sso2509 is a translation recovery factor (Trf) that interacts with aIF2 and is responsible for the release of aIF2 from bound mRNAs, thereby enabling translation re-initiation. It is a member of the domain of unknown function 35 (DUF35) protein family and is conserved in Sulfolobales as well as in other archaea. Here, we present the X-ray structure of S. solfataricus Trf solved to a resolution of 1.65 Å. Trf is composed of an N-terminal rubredoxin-like domain containing a bound zinc ion and a C-terminal oligosaccharide/oligonucleotide binding fold domain. The Trf structure reveals putative mRNA binding sites in both domains. Surprisingly, the Trf protein is structurally but not sequentially very similar to proteins linked to acyl-CoA utilization-for example, the Sso2064 protein from S. solfataricus-as well as to scaffold proteins found in the acetoacetyl-CoA thiolase/high-mobility group-CoA synthase complex of the archaeon Methanothermococcus thermolithotrophicus and in a steroid side-chain-cleaving aldolase complex from the bacterium Thermomonospora curvata. This suggests that members of the DUF35 protein family are able to act as scaffolding and binding proteins in a wide variety of biological processes.
(© 2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.)
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فهرسة مساهمة: Keywords: Sulfolobus solfataricus; DUF35; ribosome; translation initiation; translation recovery factor Trf
المشرفين على المادة: 0 (Archaeal Proteins)
0 (Carrier Proteins)
0 (Peptide Initiation Factors)
0 (Prokaryotic Initiation Factors)
0 (initiation factor 2, archaeal)
تواريخ الأحداث: Date Created: 20191206 Date Completed: 20220103 Latest Revision: 20221005
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6996347
DOI: 10.1002/2211-5463.12772
PMID: 31804766
قاعدة البيانات: MEDLINE
الوصف
تدمد:2211-5463
DOI:10.1002/2211-5463.12772