دورية أكاديمية
Nanostructured Systems Improve the Antimicrobial Potential of the Essential Oil from Cymbopogon densiflorus Leaves.
| العنوان: | Nanostructured Systems Improve the Antimicrobial Potential of the Essential Oil from Cymbopogon densiflorus Leaves. |
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| المؤلفون: | Seibert JB; Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , 35400-000 , Brazil., Viegas JSR; Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , 35400-000 , Brazil., Almeida TC; Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , 35400-000 , Brazil., Amparo TR; Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , 35400-000 , Brazil., Rodrigues IV; Departamento de Farmácia , Universidade Federal de Juiz de Fora , Governador Valadares , 36010-041 , Brazil., Lanza JS; Departamento de Fisiologia e Biofísica , Universidade Federal de Minas Gerais , Belo Horizonte , 30150-260 , Brazil., Frézard FJG; Departamento de Fisiologia e Biofísica , Universidade Federal de Minas Gerais , Belo Horizonte , 30150-260 , Brazil., Soares RDOA; Núcleo de Pesquisas em Ciências Biológicas , Universidade Federal de Ouro Preto , Ouro Preto , 35400-000 , Brazil., Teixeira LFM; Departamento de Análises Clínicas , Universidade Federal de Ouro Preto , Ouro Preto , 35400-000 , Brazil., de Souza GHB; Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , 35400-000 , Brazil., Vieira PMA; Departamento de Ciências Biológicas , Universidade Federal de Ouro Preto , Ouro Preto , 35400-000 , Brazil., Barichello JM; Departamento de Farmácia , Universidade Federal de Pelotas , Pelotas , 96020-000 , Brazil., Dos Santos ODH; Departamento de Farmácia , Universidade Federal de Ouro Preto , Ouro Preto , 35400-000 , Brazil. |
| المصدر: | Journal of natural products [J Nat Prod] 2019 Dec 27; Vol. 82 (12), pp. 3208-3220. Date of Electronic Publication: 2019 Dec 09. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society of Pharmacognosy Country of Publication: United States NLM ID: 7906882 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6025 (Electronic) Linking ISSN: 01633864 NLM ISO Abbreviation: J Nat Prod Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cincinnati, American Society of Pharmacognosy. |
| مواضيع طبية MeSH: | Nanostructures*, Anti-Infective Agents/*pharmacology , Cymbopogon/*chemistry , Oils, Volatile/*pharmacology , Plant Leaves/*chemistry, Animals ; Anti-Infective Agents/chemistry ; Bacteria/drug effects ; Cell Line ; Cell Membrane Permeability/drug effects ; Drug Screening Assays, Antitumor ; Fungi/drug effects ; Gas Chromatography-Mass Spectrometry ; Hydrogen-Ion Concentration ; Mice ; Microbial Sensitivity Tests ; Microscopy, Electron, Transmission ; Oils, Volatile/chemistry ; Particle Size ; Quantitative Structure-Activity Relationship |
| مستخلص: | The physicochemical characteristics of nanostructured suspensions are important prerequisites for the success of new drug development. This work aimed to develop nanometric systems containing Cymbopogon densiflorus leaf essential oil and to evaluate their antimicrobial activity. The essential oil was isolated by hydrodistillation from leaves and analyzed by GC-MS. The main constituents were found to be trans - p -mentha-2,8-dien-1-ol, cis - p -mentha-2,8-dien-1-ol, trans - p -mentha-1(7),8-dien-2-ol, cis -piperitol, and cis - p -mentha-1(7),8-dien-2-ol. In silico prediction analysis suggested that this oil possesses antimicrobial potential and the main mechanism of action might be the peptidoglycan glycosyltransferase inhibition. Nanoemulsions were prepared by the phase inversion method, and liposomes were made by the film hydration method. Qualitative evaluation of the antimicrobial activity was performed by the diffusion disk assay with 24 microorganisms; all of them were found to be sensitive to the essential oil. Subsequently, this property was quantified by the serial microdilution technique, where the nanoformulations demonstrated improved activity in comparison with the free oil. Bactericidal action was tested by the propidium iodide method, which revealed that free essential oil and nanoemulsion increased cytoplasmic membrane permeability, while no difference was observed between negative control and liposome. These results were confirmed by images obtained using transmission electron microscopy. This study has shown an optimization in the antimicrobial activity of C. densiflorus essential oil by a nanoemulsion and a liposomal formulation of the active substances. |
| المشرفين على المادة: | 0 (Anti-Infective Agents) 0 (Oils, Volatile) |
| تواريخ الأحداث: | Date Created: 20191210 Date Completed: 20201112 Latest Revision: 20201112 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1021/acs.jnatprod.8b00870 |
| PMID: | 31815454 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-6025 |
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| DOI: | 10.1021/acs.jnatprod.8b00870 |