دورية أكاديمية
أعرض في EDS

Multi-omics profiling of mouse gastrulation at single-cell resolution.

التفاصيل البيبلوغرافية
العنوان: Multi-omics profiling of mouse gastrulation at single-cell resolution.
المؤلفون: Argelaguet R; European Bioinformatics Institute (EMBL-EBI), Cambridge, UK., Clark SJ; Epigenetics Programme, Babraham Institute, Cambridge, UK. stephen.clark@babraham.ac.uk., Mohammed H; Epigenetics Programme, Babraham Institute, Cambridge, UK., Stapel LC; Epigenetics Programme, Babraham Institute, Cambridge, UK., Krueger C; Epigenetics Programme, Babraham Institute, Cambridge, UK., Kapourani CA; School of Informatics, University of Edinburgh, Edinburgh, UK.; MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK., Imaz-Rosshandler I; Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.; Department of Haematology, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK., Lohoff T; Epigenetics Programme, Babraham Institute, Cambridge, UK.; Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK., Xiang Y; Center for Stem Cell Biology and Regenerative Medicine, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China.; THU-PKU Center for Life Sciences, Tsinghua University, Beijing, China., Hanna CW; Epigenetics Programme, Babraham Institute, Cambridge, UK.; Centre for Trophoblast Research, University of Cambridge, Cambridge, UK., Smallwood S; Epigenetics Programme, Babraham Institute, Cambridge, UK., Ibarra-Soria X; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK., Buettner F; Helmholtz Zentrum München-German Research Center for Environmental Health, Institute of Computational Biology, Neuherberg, Germany., Sanguinetti G; School of Informatics, University of Edinburgh, Edinburgh, UK., Xie W; Center for Stem Cell Biology and Regenerative Medicine, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China.; THU-PKU Center for Life Sciences, Tsinghua University, Beijing, China., Krueger F; Bioinformatics Group, Babraham Institute, Cambridge, UK., Göttgens B; Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.; Department of Haematology, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK., Rugg-Gunn PJ; Epigenetics Programme, Babraham Institute, Cambridge, UK.; Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.; Department of Haematology, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.; Centre for Trophoblast Research, University of Cambridge, Cambridge, UK., Kelsey G; Epigenetics Programme, Babraham Institute, Cambridge, UK.; Centre for Trophoblast Research, University of Cambridge, Cambridge, UK., Dean W; Department of Biochemistry and Molecular Biology, Alberta Children's Hospital Research Institute, University of Calgary, Calgary, Alberta, Canada., Nichols J; Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK., Stegle O; European Bioinformatics Institute (EMBL-EBI), Cambridge, UK. o.stegle@dkfz.de.; European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. o.stegle@dkfz.de.; Division of Computational Genomics and Systems Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany. o.stegle@dkfz.de., Marioni JC; European Bioinformatics Institute (EMBL-EBI), Cambridge, UK. john.marioni@cruk.cam.ac.uk.; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK. john.marioni@cruk.cam.ac.uk.; Wellcome Sanger Institute, Cambridge, UK. john.marioni@cruk.cam.ac.uk., Reik W; Epigenetics Programme, Babraham Institute, Cambridge, UK. wolf.reik@babraham.ac.uk.; Centre for Trophoblast Research, University of Cambridge, Cambridge, UK. wolf.reik@babraham.ac.uk.; Wellcome Sanger Institute, Cambridge, UK. wolf.reik@babraham.ac.uk.
المصدر: Nature [Nature] 2019 Dec; Vol. 576 (7787), pp. 487-491. Date of Electronic Publication: 2019 Dec 11.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 0410462 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-4687 (Electronic) Linking ISSN: 00280836 NLM ISO Abbreviation: Nature Subsets: MEDLINE
أسماء مطبوعة: Publication: Basingstoke : Nature Publishing Group
Original Publication: London, Macmillan Journals ltd.
مواضيع طبية MeSH: DNA Methylation* , Epigenesis, Genetic* , Gene Expression Regulation, Developmental* , Single-Cell Analysis*, Gastrula/*cytology , Gastrula/*metabolism , Gastrulation/*genetics , RNA/*genetics, Animals ; Cell Differentiation/genetics ; Cell Lineage/genetics ; Chromatin/genetics ; Chromatin/metabolism ; Demethylation ; Embryoid Bodies/cytology ; Endoderm/cytology ; Endoderm/embryology ; Endoderm/metabolism ; Enhancer Elements, Genetic/genetics ; Epigenome/genetics ; Erythropoiesis ; Factor Analysis, Statistical ; Gastrula/embryology ; Gastrulation/physiology ; Mesoderm/cytology ; Mesoderm/embryology ; Mesoderm/metabolism ; Mice ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/metabolism ; RNA/analysis ; Time Factors ; Zinc Fingers
مستخلص: Formation of the three primary germ layers during gastrulation is an essential step in the establishment of the vertebrate body plan and is associated with major transcriptional changes 1-5 . Global epigenetic reprogramming accompanies these changes 6-8 , but the role of the epigenome in regulating early cell-fate choice remains unresolved, and the coordination between different molecular layers is unclear. Here we describe a single-cell multi-omics map of chromatin accessibility, DNA methylation and RNA expression during the onset of gastrulation in mouse embryos. The initial exit from pluripotency coincides with the establishment of a global repressive epigenetic landscape, followed by the emergence of lineage-specific epigenetic patterns during gastrulation. Notably, cells committed to mesoderm and endoderm undergo widespread coordinated epigenetic rearrangements at enhancer marks, driven by ten-eleven translocation (TET)-mediated demethylation and a concomitant increase of accessibility. By contrast, the methylation and accessibility landscape of ectodermal cells is already established in the early epiblast. Hence, regulatory elements associated with each germ layer are either epigenetically primed or remodelled before cell-fate decisions, providing the molecular framework for a hierarchical emergence of the primary germ layers.
التعليقات: Comment in: Nat Rev Genet. 2020 Mar;21(3):134-135. (PMID: 31925408)
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معلومات مُعتمدة: 095645 United Kingdom WT_ Wellcome Trust; 105031 United Kingdom WT_ Wellcome Trust; MR/K011332/1 United Kingdom MRC_ Medical Research Council; 22231 United Kingdom CRUK_ Cancer Research UK; 108438/E/15/Z United Kingdom WT_ Wellcome Trust; United Kingdom WT_ Wellcome Trust; 810296 International ERC_ European Research Council; MC_PC_12009 United Kingdom MRC_ Medical Research Council; MR/M01536X/1 United Kingdom MRC_ Medical Research Council; MC_UU_00009/2 United Kingdom MRC_ Medical Research Council; MC_UU_00009/1 United Kingdom MRC_ Medical Research Council; 210754/Z/18/Z United Kingdom WT_ Wellcome Trust
المشرفين على المادة: 0 (Chromatin)
63231-63-0 (RNA)
تواريخ الأحداث: Date Created: 20191213 Date Completed: 20200427 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC6924995
DOI: 10.1038/s41586-019-1825-8
PMID: 31827285
قاعدة البيانات: MEDLINE
الوصف
تدمد:1476-4687
DOI:10.1038/s41586-019-1825-8